Q9HM33 (DP2L_THEAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase II large subunit
Short name=Pol II
EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	Ta0036

Organism

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

Taxonomic identifier

273075 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	1087 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	DNA catabolic process Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1087	1087	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000152580

Sequences

	Sequence		Length	Mass (Da)
	Q9HM33 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 9DACD6508846857D	FASTA	1,087	122,284
	10 20 30 40 50 60 MIMSQKPFDL EGYRKYITEK VREAFNVAQE ARAKGLDVSD HVEIPLASDM AERIEALIGI 70 80 90 100 110 120 KGIAQEIRDL SSRMSREEVS LEMSRRIAAM FKDNRKEALD KAIRVGLAIL TEGILVAPLE 130 140 150 160 170 180 GIADVYIGKN QDGSEYVGIS YAGPIRGAGG TAQALSVLIG DVVRRELGIS RFQPTEDEIE 190 200 210 220 230 240 RYIEEIESYD RIKHLQYMPT PDEIKLVVRN SPICIDGEGS EEEEVSGHRD MERIKTNRIR 250 260 270 280 290 300 GGMCLVLCEG LVQKARKILK YTSSMHLDDW NFLANLGGKA EGKSSKKSDK FLKDIVAGRP 310 320 330 340 350 360 VFSHPSRPGG FRLRYGRSRV SGLAAASLNP ATMYIMGKFI AIGSQIKVEL PGKAAAVTPC 370 380 390 400 410 420 DTIDGPTVLL KNGDHVKIND IEKAREVYDD VVEITDAGEI LIAYGDFLEN NYPLPTPSFT 430 440 450 460 470 480 VEWWEQYLPD GVNAKDIDQF SAVEISRKYG IPLHPYYDYY WHDISFEDLE FLVKNAEQWS 490 500 510 520 530 540 ITEDGMRVPY PAFDVFIRLG IEFRRSGDYL IIRDYYPLLI SLGYDVRNGK IVNVKKYERK 550 560 570 580 590 600 GSVMETVNYL SGLIIKPRAP TRVGSRLGRP EKAGDRKMKP MVHSLFPVES YGEARRSIIG 610 620 630 640 650 660 ANKNSEGSYK AEVFFYRCNS CGFETPTPVC PRCGGHCSPL GEKTGSIDLE SILNRAESIL 670 680 690 700 710 720 GISLDSLKEF KGVKKLMSKE KVAEPIEKGI LRAVHDISVN KDGTCRFDMS DIPITHFRYR 730 740 750 760 770 780 EIGIDERTLA DLGYEVRDVN ELFPQDVIIP RKAAKYLFNV SRFIDDLLVK YYNMPPFYSL 790 800 810 820 830 840 ESEEDLIGHL IIGLAPHTSG GVVGRIIGFS DVNAFYAHPF FHAAKRRNCD GDEDSVMLLM 850 860 870 880 890 900 DGFLNFSARY LPSTRGGLMD APLVLSVLIN PDEIDKEALN VDTLSRYPVL FYEAAERHAS 910 920 930 940 950 960 PAEIEDTMMT MKVRIKKTGT YMGSSYTMDT SDINSGVLVS SYKTLGTMDE KINEQLGLAK 970 980 990 1000 1010 1020 KLRAVDADDV AARVISTHFL PDMYGNFRKF FSQEFRCTKC NAKYRRIPLS GRCQKCGSTS 1030 1040 1050 1060 1070 1080 LTLTIHKGSV VKYLNETLKI AENYRLPDYL KARIDNLART IKETFPDTEE EEKPEPREVK ITGLDMY

References

[1]

"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL445063 Genomic DNA. Translation: CAC11185.1.
RefSeq	NP_393515.1. NC_002578.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1456960.
GenomeReviews	Gene locus Ta0036 in contig AL139299_GR.
NMPDR	fig|273075.1.peg.37.
Phylogenomic databases
HOGENOM	HBG497074.
OMA	NCDGDED.
ProtClustDB	PRK04023.
Enzyme and pathway databases
BioCyc	TACI273075:TA0036-MONOMER.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. PolC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_THEAC

Accession

Primary (citable) accession number: Q9HM33

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	March 1, 2001
Last modified:	October 19, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program