Q9HM15 (PYRE_THEAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	Ta0056

Organism

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

Taxonomic identifier

273075 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 171	171	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110792
Regions
	Region	110 – 118	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	85	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	86	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	88	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	90	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	114	1	Orotate By similarity
	Binding site	142	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HM15 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 50C1ACF065B0E6D2	FASTA	171	18,641
	10 20 30 40 50 60 MGGLEEDLLR VGAIKFGDFV LTSGKRSTYY VDIKEAATDP SVLRKIASEF SGMIRSSKIA 70 80 90 100 110 120 GMELGAVPLI VATALQMSIP YIIVRKERSH GTMSLLVGKF EKGEEIDVIE DVVTTGNSVL 130 140 150 160 170 KAVNTLRENG AVVKRAYCVV DREEGGSDLL KENGIDLHPI IRISQVKGFR K

References

[1]

"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL445063 Genomic DNA. Translation: CAC11204.1.
RefSeq	NP_393535.1. NC_002578.1.
3D structure databases
ProteinModelPortal	Q9HM15.
ModBase	Search...
Proteomic databases
PRIDE	Q9HM15.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1455719.
GenomeReviews	Gene locus Ta0056 in contig AL139299_GR.
KEGG	tac:Ta0056.
NMPDR	fig|273075.1.peg.57.
Phylogenomic databases
HOGENOM	HBG404341.
OMA	QTCLVIN.
PhylomeDB	Q9HM15.
ProtClustDB	PRK00455.
Enzyme and pathway databases
BioCyc	TACI273075:TA0056-MONOMER.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. PyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_THEAC

Accession

Primary (citable) accession number: Q9HM15

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program