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Q9HM08

- TOP1_THEAC

UniProt

Q9HM08 - TOP1_THEAC

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Protein

DNA topoisomerase 1

Gene

topA

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi10 – 101Magnesium 1; catalyticUniRule annotation
Sitei55 – 551Interaction with DNAUniRule annotation
Metal bindingi109 – 1091Magnesium 1; catalyticUniRule annotation
Metal bindingi109 – 1091Magnesium 2UniRule annotation
Metal bindingi111 – 1111Magnesium 2UniRule annotation
Sitei166 – 1661Interaction with DNAUniRule annotation
Sitei170 – 1701Interaction with DNAUniRule annotation
Active sitei312 – 3121O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei314 – 3141Interaction with DNAUniRule annotation
Sitei497 – 4971Interaction with DNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri611 – 63828C4-type 1Add
BLAST
Zinc fingeri673 – 70028C4-type 2Add
BLAST
Zinc fingeri719 – 74426C4-type 3Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:Ta0063
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
ProteomesiUP000001024: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 770770DNA topoisomerase 1PRO_0000145182Add
BLAST

Proteomic databases

PRIDEiQ9HM08.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi273075.Ta0063.

Structurei

3D structure databases

ProteinModelPortaliQ9HM08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 140137ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni194 – 1996Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri611 – 63828C4-type 1Add
BLAST
Zinc fingeri673 – 70028C4-type 2Add
BLAST
Zinc fingeri719 – 74426C4-type 3Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000004019.
KOiK03168.
OMAiFCPDAEY.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HM08-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDGFRIIIAE KADAGRRIAY FLSGGQVKSH RAKGTSYLEF EYNGSKTYLI
60 70 80 90 100
PLSGHIVEAD FESGYSDWNK IDLSDLIDAR IVKNIKNKVA YQTLQAFRGK
110 120 130 140 150
VEEIVIATDY DREGELIGVE ALDIIKEGKE EIRRAKFSAL TKNEILDSFK
160 170 180 190 200
NLIGVNYSLA DAADARESID LIWGSVLTRF FSVTTGRLGK SFLSAGRVQT
210 220 230 240 250
PTLAIVVDRE REIQSFRPER YWTISITFDK DGQFKARYPE NIKDQDTAEK
260 270 280 290 300
IYEAIKGKNG RVSSYTSKED HIRRPAPFST TEFLREASRI GIMPTKAMSI
310 320 330 340 350
AENLYMRGLI SYPRTDNTVY PRSINLKSVL KKLENTAYSK YVKEIETFDR
360 370 380 390 400
ILPSRGRIET TDHPPIYPVD SPKEQLKGDY GRVYDLILRH FLSTLYRDGK
410 420 430 440 450
KTVAEAEIYV NGYTFKAAGQ HTTDRGWTEI YGYDPKDVYL PELTEGEDLK
460 470 480 490 500
AIDWNIQREE TKPPPRYDMS SLLKKMEELN LGTKSTRHDI IGKLIERGFI
510 520 530 540 550
EGNPVKPTPL GMAFIDAVRS VNSHIADPEM TAKLEEDMDR IEKNEMSKND
560 570 580 590 600
VVEESKKMLH EVLSHFLTKT ADVKDIITKG INAGQEIGDC PFHEGKKIMV
610 620 630 640 650
IRDRFTYTVR CEDPSCKINF RIKRNGSITL SDQKCPVCGL PMIKIIRKGQ
660 670 680 690 700
SPEIKCIDPD CSYNRENEDY GECPADHGRL VLRQSKYGKR FLGCSNYPKC
710 720 730 740 750
TVTYPLPQMG RITKTGEVCP YCGAPILALS RNGRKWKFCP NMQCEYNKKR
760 770
KPEAAVEKSV RKKGRNASKS
Length:770
Mass (Da):87,667
Last modified:March 1, 2001 - v1
Checksum:i75DA8DD7BC3B8A22
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL445063 Genomic DNA. Translation: CAC11211.1.
RefSeqiNP_393542.1. NC_002578.1.
WP_010900491.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC11211; CAC11211; CAC11211.
GeneIDi1455725.
KEGGitac:Ta0063.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL445063 Genomic DNA. Translation: CAC11211.1 .
RefSeqi NP_393542.1. NC_002578.1.
WP_010900491.1. NC_002578.1.

3D structure databases

ProteinModelPortali Q9HM08.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273075.Ta0063.

Proteomic databases

PRIDEi Q9HM08.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC11211 ; CAC11211 ; CAC11211 .
GeneIDi 1455725.
KEGGi tac:Ta0063.

Phylogenomic databases

eggNOGi COG0551.
HOGENOMi HOG000004019.
KOi K03168.
OMAi FCPDAEY.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
    Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
    Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Entry informationi

Entry nameiTOP1_THEAC
AccessioniPrimary (citable) accession number: Q9HM08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3