Q9HM08 (TOP1_THEAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 1
EC=5.99.1.2

Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme

Gene names

Name:	topA
Ordered Locus Names:	Ta0063

Organism

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

Taxonomic identifier

273075 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	770 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the type IA topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 770	770	DNA topoisomerase 1		PRO_0000145182
Regions
	Domain	4 – 138	135	Toprim
	Zinc finger	611 – 638	28	C4-type 1
	Zinc finger	673 – 700	28	C4-type 2
	Zinc finger	719 – 744	26	C4-type 3
Sites
	Active site	312	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HM08 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 75DA8DD7BC3B8A22	FASTA	770	87,667
	10 20 30 40 50 60 MDGFRIIIAE KADAGRRIAY FLSGGQVKSH RAKGTSYLEF EYNGSKTYLI PLSGHIVEAD 70 80 90 100 110 120 FESGYSDWNK IDLSDLIDAR IVKNIKNKVA YQTLQAFRGK VEEIVIATDY DREGELIGVE 130 140 150 160 170 180 ALDIIKEGKE EIRRAKFSAL TKNEILDSFK NLIGVNYSLA DAADARESID LIWGSVLTRF 190 200 210 220 230 240 FSVTTGRLGK SFLSAGRVQT PTLAIVVDRE REIQSFRPER YWTISITFDK DGQFKARYPE 250 260 270 280 290 300 NIKDQDTAEK IYEAIKGKNG RVSSYTSKED HIRRPAPFST TEFLREASRI GIMPTKAMSI 310 320 330 340 350 360 AENLYMRGLI SYPRTDNTVY PRSINLKSVL KKLENTAYSK YVKEIETFDR ILPSRGRIET 370 380 390 400 410 420 TDHPPIYPVD SPKEQLKGDY GRVYDLILRH FLSTLYRDGK KTVAEAEIYV NGYTFKAAGQ 430 440 450 460 470 480 HTTDRGWTEI YGYDPKDVYL PELTEGEDLK AIDWNIQREE TKPPPRYDMS SLLKKMEELN 490 500 510 520 530 540 LGTKSTRHDI IGKLIERGFI EGNPVKPTPL GMAFIDAVRS VNSHIADPEM TAKLEEDMDR 550 560 570 580 590 600 IEKNEMSKND VVEESKKMLH EVLSHFLTKT ADVKDIITKG INAGQEIGDC PFHEGKKIMV 610 620 630 640 650 660 IRDRFTYTVR CEDPSCKINF RIKRNGSITL SDQKCPVCGL PMIKIIRKGQ SPEIKCIDPD 670 680 690 700 710 720 CSYNRENEDY GECPADHGRL VLRQSKYGKR FLGCSNYPKC TVTYPLPQMG RITKTGEVCP 730 740 750 760 770 YCGAPILALS RNGRKWKFCP NMQCEYNKKR KPEAAVEKSV RKKGRNASKS

References

[1]

"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL445063 Genomic DNA. Translation: CAC11211.1.
RefSeq	NP_393542.1. NC_002578.1.
3D structure databases
ProteinModelPortal	Q9HM08.
ModBase	Search...
Proteomic databases
PRIDE	Q9HM08.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1455725.
GenomeReviews	Gene locus Ta0063 in contig AL139299_GR.
KEGG	tac:Ta0063.
NMPDR	fig|273075.1.peg.64.
Phylogenomic databases
HOGENOM	HBG512935.
OMA	QVIDLVW.
PhylomeDB	Q9HM08.
ProtClustDB	PRK07219.
Enzyme and pathway databases
BioCyc	TACI273075:TA0063-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_THEAC

Accession

Primary (citable) accession number: Q9HM08

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program