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Q9HM08

- TOP1_THEAC

UniProt

Q9HM08 - TOP1_THEAC

Protein

DNA topoisomerase 1

Gene

topA

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi10 – 101Magnesium 1; catalyticUniRule annotation
    Sitei55 – 551Interaction with DNAUniRule annotation
    Metal bindingi109 – 1091Magnesium 1; catalyticUniRule annotation
    Metal bindingi109 – 1091Magnesium 2UniRule annotation
    Metal bindingi111 – 1111Magnesium 2UniRule annotation
    Sitei166 – 1661Interaction with DNAUniRule annotation
    Sitei170 – 1701Interaction with DNAUniRule annotation
    Active sitei312 – 3121O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei314 – 3141Interaction with DNAUniRule annotation
    Sitei497 – 4971Interaction with DNAUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri611 – 63828C4-type 1Add
    BLAST
    Zinc fingeri673 – 70028C4-type 2Add
    BLAST
    Zinc fingeri719 – 74426C4-type 3Add
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:Ta0063
    OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
    Taxonomic identifieri273075 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
    ProteomesiUP000001024: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 770770DNA topoisomerase 1PRO_0000145182Add
    BLAST

    Proteomic databases

    PRIDEiQ9HM08.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi273075.Ta0063.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HM08.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 140137ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni194 – 1996Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri611 – 63828C4-type 1Add
    BLAST
    Zinc fingeri673 – 70028C4-type 2Add
    BLAST
    Zinc fingeri719 – 74426C4-type 3Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0551.
    HOGENOMiHOG000004019.
    KOiK03168.
    OMAiFCPDAEY.

    Family and domain databases

    Gene3Di1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HM08-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDGFRIIIAE KADAGRRIAY FLSGGQVKSH RAKGTSYLEF EYNGSKTYLI    50
    PLSGHIVEAD FESGYSDWNK IDLSDLIDAR IVKNIKNKVA YQTLQAFRGK 100
    VEEIVIATDY DREGELIGVE ALDIIKEGKE EIRRAKFSAL TKNEILDSFK 150
    NLIGVNYSLA DAADARESID LIWGSVLTRF FSVTTGRLGK SFLSAGRVQT 200
    PTLAIVVDRE REIQSFRPER YWTISITFDK DGQFKARYPE NIKDQDTAEK 250
    IYEAIKGKNG RVSSYTSKED HIRRPAPFST TEFLREASRI GIMPTKAMSI 300
    AENLYMRGLI SYPRTDNTVY PRSINLKSVL KKLENTAYSK YVKEIETFDR 350
    ILPSRGRIET TDHPPIYPVD SPKEQLKGDY GRVYDLILRH FLSTLYRDGK 400
    KTVAEAEIYV NGYTFKAAGQ HTTDRGWTEI YGYDPKDVYL PELTEGEDLK 450
    AIDWNIQREE TKPPPRYDMS SLLKKMEELN LGTKSTRHDI IGKLIERGFI 500
    EGNPVKPTPL GMAFIDAVRS VNSHIADPEM TAKLEEDMDR IEKNEMSKND 550
    VVEESKKMLH EVLSHFLTKT ADVKDIITKG INAGQEIGDC PFHEGKKIMV 600
    IRDRFTYTVR CEDPSCKINF RIKRNGSITL SDQKCPVCGL PMIKIIRKGQ 650
    SPEIKCIDPD CSYNRENEDY GECPADHGRL VLRQSKYGKR FLGCSNYPKC 700
    TVTYPLPQMG RITKTGEVCP YCGAPILALS RNGRKWKFCP NMQCEYNKKR 750
    KPEAAVEKSV RKKGRNASKS 770
    Length:770
    Mass (Da):87,667
    Last modified:March 1, 2001 - v1
    Checksum:i75DA8DD7BC3B8A22
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL445063 Genomic DNA. Translation: CAC11211.1.
    RefSeqiNP_393542.1. NC_002578.1.
    WP_010900491.1. NC_002578.1.

    Genome annotation databases

    EnsemblBacteriaiCAC11211; CAC11211; CAC11211.
    GeneIDi1455725.
    KEGGitac:Ta0063.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL445063 Genomic DNA. Translation: CAC11211.1 .
    RefSeqi NP_393542.1. NC_002578.1.
    WP_010900491.1. NC_002578.1.

    3D structure databases

    ProteinModelPortali Q9HM08.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273075.Ta0063.

    Proteomic databases

    PRIDEi Q9HM08.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC11211 ; CAC11211 ; CAC11211 .
    GeneIDi 1455725.
    KEGGi tac:Ta0063.

    Phylogenomic databases

    eggNOGi COG0551.
    HOGENOMi HOG000004019.
    KOi K03168.
    OMAi FCPDAEY.

    Family and domain databases

    Gene3Di 1.10.290.10. 1 hit.
    1.10.460.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013826. Topo_IA_cen_sub3.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
      Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
      Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

    Entry informationi

    Entry nameiTOP1_THEAC
    AccessioniPrimary (citable) accession number: Q9HM08
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3