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Q9HM08 (TOP1_THEAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:Ta0063
OrganismThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) [Reference proteome] [HAMAP]
Taxonomic identifier273075 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma

Protein attributes

Sequence length770 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 770770DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145182

Regions

Domain4 – 140137Toprim
Zinc finger611 – 63828C4-type 1 HAMAP-Rule MF_00952
Zinc finger673 – 70028C4-type 2 HAMAP-Rule MF_00952
Zinc finger719 – 74426C4-type 3 HAMAP-Rule MF_00952
Region194 – 1996Interaction with DNA By similarity

Sites

Active site3121O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding101Magnesium 1; catalytic By similarity
Metal binding1091Magnesium 1; catalytic By similarity
Metal binding1091Magnesium 2 By similarity
Metal binding1111Magnesium 2 By similarity
Site551Interaction with DNA By similarity
Site1661Interaction with DNA By similarity
Site1701Interaction with DNA By similarity
Site3141Interaction with DNA By similarity
Site4971Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9HM08 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 75DA8DD7BC3B8A22

FASTA77087,667
        10         20         30         40         50         60 
MDGFRIIIAE KADAGRRIAY FLSGGQVKSH RAKGTSYLEF EYNGSKTYLI PLSGHIVEAD 

        70         80         90        100        110        120 
FESGYSDWNK IDLSDLIDAR IVKNIKNKVA YQTLQAFRGK VEEIVIATDY DREGELIGVE 

       130        140        150        160        170        180 
ALDIIKEGKE EIRRAKFSAL TKNEILDSFK NLIGVNYSLA DAADARESID LIWGSVLTRF 

       190        200        210        220        230        240 
FSVTTGRLGK SFLSAGRVQT PTLAIVVDRE REIQSFRPER YWTISITFDK DGQFKARYPE 

       250        260        270        280        290        300 
NIKDQDTAEK IYEAIKGKNG RVSSYTSKED HIRRPAPFST TEFLREASRI GIMPTKAMSI 

       310        320        330        340        350        360 
AENLYMRGLI SYPRTDNTVY PRSINLKSVL KKLENTAYSK YVKEIETFDR ILPSRGRIET 

       370        380        390        400        410        420 
TDHPPIYPVD SPKEQLKGDY GRVYDLILRH FLSTLYRDGK KTVAEAEIYV NGYTFKAAGQ 

       430        440        450        460        470        480 
HTTDRGWTEI YGYDPKDVYL PELTEGEDLK AIDWNIQREE TKPPPRYDMS SLLKKMEELN 

       490        500        510        520        530        540 
LGTKSTRHDI IGKLIERGFI EGNPVKPTPL GMAFIDAVRS VNSHIADPEM TAKLEEDMDR 

       550        560        570        580        590        600 
IEKNEMSKND VVEESKKMLH EVLSHFLTKT ADVKDIITKG INAGQEIGDC PFHEGKKIMV 

       610        620        630        640        650        660 
IRDRFTYTVR CEDPSCKINF RIKRNGSITL SDQKCPVCGL PMIKIIRKGQ SPEIKCIDPD 

       670        680        690        700        710        720 
CSYNRENEDY GECPADHGRL VLRQSKYGKR FLGCSNYPKC TVTYPLPQMG RITKTGEVCP 

       730        740        750        760        770 
YCGAPILALS RNGRKWKFCP NMQCEYNKKR KPEAAVEKSV RKKGRNASKS 

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References

[1]"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL445063 Genomic DNA. Translation: CAC11211.1.
RefSeqNP_393542.1. NC_002578.1.

3D structure databases

ProteinModelPortalQ9HM08.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273075.Ta0063.

Proteomic databases

PRIDEQ9HM08.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC11211; CAC11211; CAC11211.
GeneID1455725.
KEGGtac:Ta0063.

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000004019.
KOK03168.
OMAFCPDAEY.
ProtClustDBPRK07219.

Family and domain databases

Gene3D1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_THEAC
AccessionPrimary (citable) accession number: Q9HM08
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: March 1, 2001
Last modified: February 19, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families