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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75SubstrateUniRule annotation1
Metal bindingi216ManganeseUniRule annotation1
Metal bindingi236Manganese; via tele nitrogenUniRule annotation1
Binding sitei258SubstrateUniRule annotation1
Active sitei260UniRule annotation1
Metal bindingi287ManganeseUniRule annotation1
Binding sitei376GTPUniRule annotation1
Binding sitei407GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi259 – 264GTPUniRule annotation6
Nucleotide bindingi500 – 503GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTACI273075:G1GT7-126-MONOMER
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Ta0123
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001024 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036241 – 588Phosphoenolpyruvate carboxykinase [GTP]Add BLAST588

Interactioni

Protein-protein interaction databases

STRINGi273075.Ta0123

Structurei

3D structure databases

ProteinModelPortaliQ9HLV2
SMRiQ9HLV2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni207 – 209Substrate bindingUniRule annotation3
Regioni374 – 376Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG05865 Archaea
COG1274 LUCA
HOGENOMiHOG000191700
KOiK01596
OMAiCWLALTD
OrthoDBiPOG093Z01SN

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HLV2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAIDESELN QHAMKWIEGI KKFTEAEDVV VCDGTPEEFK QISNELIKSG
60 70 80 90 100
EFIKLNENRY PNSFLYRSDR TDVARSEERT FIAAPDASMA GSLNNHMTLQ
110 120 130 140 150
QVSEVWNKFF RGAYRGKTMF VIPYALGPLN SRFTDYGIEI TDSRYVVLNL
160 170 180 190 200
HYITRMGKQV IGSMPEKFVK GVHATGTLDP GNKFIIHIPW DKPEGVDADI
210 220 230 240 250
LSVNTNYGGN ALLSKKCHAL RIASVRARKE GWLAEHMLLL EVEDPHGRKV
260 270 280 290 300
YITGAFPSAS GKTNLAMINP PKQYAEAGWK TRLLSDDIAW MKMKDGMLYA
310 320 330 340 350
TNPENGFFAV VPGTNYRTNK NAMITLSRNT IFTNTGMTKT GEPWWEGLDP
360 370 380 390 400
LQEELYDWKG VLRKPDGEPI AHPNSRFTSP LSNYPFLSDR SEDPEGVPVS
410 420 430 440 450
AILFGGRRAS LVPLVYEAFN WNHGVFMGAT MGVEKTAASE GKVGELRRDP
460 470 480 490 500
MAMRPFCGYN ISDYFRHWIE MGRKLSRRPK IFYVNWFRRR QDGSFIWPGF
510 520 530 540 550
SENFRVIEWI LYRLDHNDNA IETPIGYIPE NINTDGLNLT KQDMEELFRI
560 570 580
DRDGWREEMK SIGDYFSQIG NIPEDLLIEF EMEKRRIS
Length:588
Mass (Da):67,308
Last modified:September 26, 2001 - v2
Checksum:i30E963E7E62D78C1
GO

Sequence cautioni

The sequence CAC11270 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445063 Genomic DNA Translation: CAC11270.1 Different initiation.
RefSeqiWP_048162215.1, NC_002578.1

Genome annotation databases

EnsemblBacteriaiCAC11270; CAC11270; CAC11270
GeneIDi1455774
KEGGitac:Ta0123

Similar proteinsi

Entry informationi

Entry nameiPCKG_THEAC
AccessioniPrimary (citable) accession number: Q9HLV2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: March 28, 2018
This is version 98 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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