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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathway: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751SubstrateUniRule annotation
Binding sitei209 – 2091Substrate; via amide nitrogenUniRule annotation
Metal bindingi216 – 2161ManganeseUniRule annotation
Binding sitei216 – 2161SubstrateUniRule annotation
Metal bindingi236 – 2361Manganese; via tele nitrogenUniRule annotation
Binding sitei258 – 2581SubstrateUniRule annotation
Active sitei260 – 2601UniRule annotation
Metal bindingi287 – 2871ManganeseUniRule annotation
Binding sitei376 – 3761GTPUniRule annotation
Binding sitei407 – 4071GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi259 – 2646GTPUniRule annotation
Nucleotide bindingi500 – 5034GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Ta0123
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
ProteomesiUP000001024 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 588588Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103624Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi273075.Ta0123.

Structurei

3D structure databases

ProteinModelPortaliQ9HLV2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni374 – 3763Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HLV2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAIDESELN QHAMKWIEGI KKFTEAEDVV VCDGTPEEFK QISNELIKSG
60 70 80 90 100
EFIKLNENRY PNSFLYRSDR TDVARSEERT FIAAPDASMA GSLNNHMTLQ
110 120 130 140 150
QVSEVWNKFF RGAYRGKTMF VIPYALGPLN SRFTDYGIEI TDSRYVVLNL
160 170 180 190 200
HYITRMGKQV IGSMPEKFVK GVHATGTLDP GNKFIIHIPW DKPEGVDADI
210 220 230 240 250
LSVNTNYGGN ALLSKKCHAL RIASVRARKE GWLAEHMLLL EVEDPHGRKV
260 270 280 290 300
YITGAFPSAS GKTNLAMINP PKQYAEAGWK TRLLSDDIAW MKMKDGMLYA
310 320 330 340 350
TNPENGFFAV VPGTNYRTNK NAMITLSRNT IFTNTGMTKT GEPWWEGLDP
360 370 380 390 400
LQEELYDWKG VLRKPDGEPI AHPNSRFTSP LSNYPFLSDR SEDPEGVPVS
410 420 430 440 450
AILFGGRRAS LVPLVYEAFN WNHGVFMGAT MGVEKTAASE GKVGELRRDP
460 470 480 490 500
MAMRPFCGYN ISDYFRHWIE MGRKLSRRPK IFYVNWFRRR QDGSFIWPGF
510 520 530 540 550
SENFRVIEWI LYRLDHNDNA IETPIGYIPE NINTDGLNLT KQDMEELFRI
560 570 580
DRDGWREEMK SIGDYFSQIG NIPEDLLIEF EMEKRRIS
Length:588
Mass (Da):67,308
Last modified:September 26, 2001 - v2
Checksum:i30E963E7E62D78C1
GO

Sequence cautioni

The sequence CAC11270.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445063 Genomic DNA. Translation: CAC11270.1. Different initiation.
RefSeqiNP_393601.1. NC_002578.1.
WP_010900550.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC11270; CAC11270; CAC11270.
GeneIDi1455774.
KEGGitac:Ta0123.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445063 Genomic DNA. Translation: CAC11270.1. Different initiation.
RefSeqiNP_393601.1. NC_002578.1.
WP_010900550.1. NC_002578.1.

3D structure databases

ProteinModelPortaliQ9HLV2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273075.Ta0123.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC11270; CAC11270; CAC11270.
GeneIDi1455774.
KEGGitac:Ta0123.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
    Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
    Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Entry informationi

Entry nameiPCKG_THEAC
AccessioniPrimary (citable) accession number: Q9HLV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: May 27, 2015
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.