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Q9HLK8

- IMDH_THEAC

UniProt

Q9HLK8 - IMDH_THEAC

Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

    Catalytic activityi

    Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

    Cofactori

    Potassium.UniRule annotation

    Enzyme regulationi

    Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei246 – 2461NADUniRule annotation
    Metal bindingi297 – 2971Potassium; via carbonyl oxygenUniRule annotation
    Metal bindingi299 – 2991Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei300 – 3001IMPUniRule annotation
    Active sitei302 – 3021Thioimidate intermediateUniRule annotation
    Metal bindingi302 – 3021Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei409 – 4091IMPUniRule annotation
    Metal bindingi463 – 4631Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi464 – 4641Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi465 – 4651Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi295 – 2973NADUniRule annotation

    GO - Molecular functioni

    1. adenyl nucleotide binding Source: InterPro
    2. IMP dehydrogenase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. GMP biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    GMP biosynthesis, Purine biosynthesis

    Keywords - Ligandi

    Metal-binding, NAD, Potassium

    Enzyme and pathway databases

    UniPathwayiUPA00601; UER00295.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
    Short name:
    IMP dehydrogenaseUniRule annotation
    Short name:
    IMPDUniRule annotation
    Short name:
    IMPDHUniRule annotation
    Gene namesi
    Name:guaBUniRule annotation
    Ordered Locus Names:Ta0219
    OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
    Taxonomic identifieri273075 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
    ProteomesiUP000001024: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 485485Inosine-5'-monophosphate dehydrogenasePRO_0000415695Add
    BLAST

    Proteomic databases

    PRIDEiQ9HLK8.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi273075.Ta0219.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HLK8.
    SMRiQ9HLK8. Positions 199-477.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini97 – 15458CBS 1UniRule annotationAdd
    BLAST
    Domaini155 – 21157CBS 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni335 – 3373IMP bindingUniRule annotation
    Regioni358 – 3592IMP bindingUniRule annotation
    Regioni382 – 3865IMP bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the IMPDH/GMPR family.UniRule annotation
    Contains 2 CBS domains.UniRule annotation

    Keywords - Domaini

    CBS domain, Repeat

    Phylogenomic databases

    eggNOGiCOG0517.
    HOGENOMiHOG000165755.
    KOiK00088.
    OMAiHGHSKNI.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01964. IMPDH.
    InterProiIPR013785. Aldolase_TIM.
    IPR000644. CBS_dom.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view]
    PANTHERiPTHR11911:SF6. PTHR11911:SF6. 1 hit.
    PfamiPF00571. CBS. 2 hits.
    PF00478. IMPDH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000130. IMPDH. 1 hit.
    SMARTiSM00116. CBS. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
    PROSITEiPS51371. CBS. 2 hits.
    PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HLK8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYREKFTKIT EGFTFDDVLL IPMRTAVEPK NVVVSSRISR HIQVKVPIVS    50
    SPMDTVTEDA MAIAMARYGA IGVIHRNQPR EKEVEMVKRV KREETIIIRD 100
    VYTISPETPI EVARTLMATR NIAGLPVVKD DKLVGIVTKR DLEFVKKGSS 150
    VSDVMVRDVI TAPENVDIDE AIEILHKNRI EKLPLVDSSG HLVGLITAKD 200
    IITRQKFPDA SRDSEGQLMV GAAVGPFDLD RAVEVEKAGA DFIVVDTAHA 250
    DNENVLSSLK KMRKQISVDI VAGNIATAQA AEDLISCDVD GLRVGIGPGS 300
    ICTTRIVAGV GVPQLTAISD VAEAAKDSGI PVIADGGIRY SGDIVKAIAA 350
    GADAVMLGSM LAGTEESPGQ EMIINGRKYK AYRGMGSIGA LTTGLSDRYS 400
    KLGNGFIAEG VEGAVPYRGR VDEVLFQLVG GLRTGMGYVG AATIEDLKRN 450
    GKFVRITNNG LRESHPHDIR LISEPPNYQI SNISP 485
    Length:485
    Mass (Da):52,225
    Last modified:March 1, 2001 - v1
    Checksum:i4450C3DC8FA68673
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL445063 Genomic DNA. Translation: CAC11365.1.
    RefSeqiNP_393697.1. NC_002578.1.
    WP_010900646.1. NC_002578.1.

    Genome annotation databases

    EnsemblBacteriaiCAC11365; CAC11365; CAC11365.
    GeneIDi1455855.
    KEGGitac:Ta0219.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL445063 Genomic DNA. Translation: CAC11365.1 .
    RefSeqi NP_393697.1. NC_002578.1.
    WP_010900646.1. NC_002578.1.

    3D structure databases

    ProteinModelPortali Q9HLK8.
    SMRi Q9HLK8. Positions 199-477.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273075.Ta0219.

    Proteomic databases

    PRIDEi Q9HLK8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC11365 ; CAC11365 ; CAC11365 .
    GeneIDi 1455855.
    KEGGi tac:Ta0219.

    Phylogenomic databases

    eggNOGi COG0517.
    HOGENOMi HOG000165755.
    KOi K00088.
    OMAi HGHSKNI.

    Enzyme and pathway databases

    UniPathwayi UPA00601 ; UER00295 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01964. IMPDH.
    InterProi IPR013785. Aldolase_TIM.
    IPR000644. CBS_dom.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view ]
    PANTHERi PTHR11911:SF6. PTHR11911:SF6. 1 hit.
    Pfami PF00571. CBS. 2 hits.
    PF00478. IMPDH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000130. IMPDH. 1 hit.
    SMARTi SM00116. CBS. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
    PROSITEi PS51371. CBS. 2 hits.
    PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
      Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
      Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

    Entry informationi

    Entry nameiIMDH_THEAC
    AccessioniPrimary (citable) accession number: Q9HLK8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 22, 2012
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3