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Q9HLK8

- IMDH_THEAC

UniProt

Q9HLK8 - IMDH_THEAC

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Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

K(+)UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei246 – 2461NADUniRule annotation
Metal bindingi297 – 2971Potassium; via carbonyl oxygenUniRule annotation
Metal bindingi299 – 2991Potassium; via carbonyl oxygenUniRule annotation
Binding sitei300 – 3001IMPUniRule annotation
Active sitei302 – 3021Thioimidate intermediateUniRule annotation
Metal bindingi302 – 3021Potassium; via carbonyl oxygenUniRule annotation
Binding sitei409 – 4091IMPUniRule annotation
Metal bindingi463 – 4631Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
Metal bindingi464 – 4641Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
Metal bindingi465 – 4651Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi295 – 2973NADUniRule annotation

GO - Molecular functioni

  1. adenyl nucleotide binding Source: InterPro
  2. IMP dehydrogenase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. GMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, Potassium

Enzyme and pathway databases

UniPathwayiUPA00601; UER00295.

Names & Taxonomyi

Protein namesi
Recommended name:
Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
Short name:
IMP dehydrogenaseUniRule annotation
Short name:
IMPDUniRule annotation
Short name:
IMPDHUniRule annotation
Gene namesi
Name:guaBUniRule annotation
Ordered Locus Names:Ta0219
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
ProteomesiUP000001024: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485Inosine-5'-monophosphate dehydrogenasePRO_0000415695Add
BLAST

Proteomic databases

PRIDEiQ9HLK8.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi273075.Ta0219.

Structurei

3D structure databases

ProteinModelPortaliQ9HLK8.
SMRiQ9HLK8. Positions 199-477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini97 – 15458CBS 1UniRule annotationAdd
BLAST
Domaini155 – 21157CBS 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni335 – 3373IMP bindingUniRule annotation
Regioni358 – 3592IMP bindingUniRule annotation
Regioni382 – 3865IMP bindingUniRule annotation

Sequence similaritiesi

Belongs to the IMPDH/GMPR family.UniRule annotation
Contains 2 CBS domains.UniRule annotation

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiCOG0517.
HOGENOMiHOG000165755.
KOiK00088.
OMAiHGHSKNI.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01964. IMPDH.
InterProiIPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PANTHERiPTHR11911:SF6. PTHR11911:SF6. 1 hit.
PfamiPF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000130. IMPDH. 1 hit.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HLK8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYREKFTKIT EGFTFDDVLL IPMRTAVEPK NVVVSSRISR HIQVKVPIVS
60 70 80 90 100
SPMDTVTEDA MAIAMARYGA IGVIHRNQPR EKEVEMVKRV KREETIIIRD
110 120 130 140 150
VYTISPETPI EVARTLMATR NIAGLPVVKD DKLVGIVTKR DLEFVKKGSS
160 170 180 190 200
VSDVMVRDVI TAPENVDIDE AIEILHKNRI EKLPLVDSSG HLVGLITAKD
210 220 230 240 250
IITRQKFPDA SRDSEGQLMV GAAVGPFDLD RAVEVEKAGA DFIVVDTAHA
260 270 280 290 300
DNENVLSSLK KMRKQISVDI VAGNIATAQA AEDLISCDVD GLRVGIGPGS
310 320 330 340 350
ICTTRIVAGV GVPQLTAISD VAEAAKDSGI PVIADGGIRY SGDIVKAIAA
360 370 380 390 400
GADAVMLGSM LAGTEESPGQ EMIINGRKYK AYRGMGSIGA LTTGLSDRYS
410 420 430 440 450
KLGNGFIAEG VEGAVPYRGR VDEVLFQLVG GLRTGMGYVG AATIEDLKRN
460 470 480
GKFVRITNNG LRESHPHDIR LISEPPNYQI SNISP
Length:485
Mass (Da):52,225
Last modified:March 1, 2001 - v1
Checksum:i4450C3DC8FA68673
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445063 Genomic DNA. Translation: CAC11365.1.
RefSeqiNP_393697.1. NC_002578.1.
WP_010900646.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC11365; CAC11365; CAC11365.
GeneIDi1455855.
KEGGitac:Ta0219.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445063 Genomic DNA. Translation: CAC11365.1 .
RefSeqi NP_393697.1. NC_002578.1.
WP_010900646.1. NC_002578.1.

3D structure databases

ProteinModelPortali Q9HLK8.
SMRi Q9HLK8. Positions 199-477.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273075.Ta0219.

Proteomic databases

PRIDEi Q9HLK8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC11365 ; CAC11365 ; CAC11365 .
GeneIDi 1455855.
KEGGi tac:Ta0219.

Phylogenomic databases

eggNOGi COG0517.
HOGENOMi HOG000165755.
KOi K00088.
OMAi HGHSKNI.

Enzyme and pathway databases

UniPathwayi UPA00601 ; UER00295 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01964. IMPDH.
InterProi IPR013785. Aldolase_TIM.
IPR000644. CBS_dom.
IPR005990. IMP_DH.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view ]
PANTHERi PTHR11911:SF6. PTHR11911:SF6. 1 hit.
Pfami PF00571. CBS. 2 hits.
PF00478. IMPDH. 1 hit.
[Graphical view ]
PIRSFi PIRSF000130. IMPDH. 1 hit.
SMARTi SM00116. CBS. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
PROSITEi PS51371. CBS. 2 hits.
PS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
    Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
    Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Entry informationi

Entry nameiIMDH_THEAC
AccessioniPrimary (citable) accession number: Q9HLK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: March 1, 2001
Last modified: November 26, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3