Q9HLK5 (DP2S_THEAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase II small subunit
Short name=Pol II
EC=2.7.7.7

Gene names

Name:	polB
Ordered Locus Names:	Ta0222

Organism

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

Taxonomic identifier

273075 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00325
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00325 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00325
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the DNA polymerase delta/II small subunit family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	DNA polymerase II small subunit HAMAP MF_00325		PRO_0000096182

Sequences

	Sequence		Length	Mass (Da)
	Q9HLK5 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 3307E418E35AC27A	FASTA	488	55,577
	10 20 30 40 50 60 MVIWFFYGFR KIMHLSLKRI IVKCKMSQLD RTSLIQYFNS NGVLISPEAL DKLIKYSSSS 70 80 90 100 110 120 LIEDLIKNSD GYIDEKYVER YVSRPKVRND YEVYLPDVRF NASIEDFRKM FVSKYEKLSK 130 140 150 160 170 180 IITSSSSMRG SISIKTAKRS QGEVKVVGMV SEVSMTKNGH KRIVIEDLDD SITAIVMKDR 190 200 210 220 230 240 GPVNEIILED EVIGIIGSVS QSSKDPVIFV NEIIRPDIPY RVIDEEKHEP VYVASISDIH 250 260 270 280 290 300 VGSKTFRKNE FEAMVRWISG SDPDASRVKY LILSGDVVDG IGVYPDQEND LEILNPLEQY 310 320 330 340 350 360 ANLAEYLVDV PEDVKVFVMP GNHDTVRLSE PQPVFPGKIR DLFEPNVAFL PNPYNLKLEG 370 380 390 400 410 420 KNVLVYHGMS LNDMIELIPG ANYDSIGKAI EAILVRRHLS PKYGGNTPMI PSAVDYHVIE 430 440 450 460 470 480 EVPDIFITGH IHSHYIGNYK GVRYVNSSTW QSQTEYQKMM NFNPKPSKLT LFDLYSRSVI VKDFDTTL

References

[1]

"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL445063 Genomic DNA. Translation: CAC11368.1.
RefSeq	NP_393700.1. NC_002578.1.
3D structure databases
ProteinModelPortal	Q9HLK5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1455858.
GenomeReviews	Gene locus Ta0222 in contig AL139299_GR.
KEGG	tac:Ta0222.
NMPDR	fig|273075.1.peg.222.
Phylogenomic databases
HOGENOM	HBG512811.
OMA	GHVHKLG.
ProtClustDB	PRK04036.
Enzyme and pathway databases
BioCyc	TACI273075:TA0222-MONOMER.
Family and domain databases
HAMAP	MF_00325. DNApol_II_A_arch. [Tree]
InterPro	IPR007185. DNA_pol_alpha/epsilon_bsu. IPR011149. Pol2_small_arc. [Graphical view]
KO	K02323.
Pfam	PF04042. DNA_pol_E_B. 1 hit. [Graphical view]
PIRSF	PIRSF000803. Arc_Pol2_small. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2S_THEAC

Accession

Primary (citable) accession number: Q9HLK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	March 1, 2001
Last modified:	November 16, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program