true2007-09-112024-03-27139KAE1B_THEACThe genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum.Ruepp A.Graml W.Santos-Martinez M.-L.Koretke K.K.Volker C.Mewes H.-W.Frishman D.Stocker S.Lupas A.N.Baumeister W.doi:10.1038/350350692000Nature407508-513NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine.Mao D.Y.Neculai D.Downey M.Orlicky S.Haffani Y.Z.Ceccarelli D.F.Ho J.S.Szilard R.K.Zhang W.Ho C.S.Wan L.Fares C.Rumpel S.Kurinov I.Arrowsmith C.H.Durocher D.Sicheri F.doi:10.1016/j.molcel.2008.10.0022008Mol. Cell32259-275X-RAY CRYSTALLOGRAPHY (3.02 ANGSTROMS) OF 1-3293.02A/B=1-329ArchaeaArchaeaTransferase(Phosphotransferase) domain 1Kae1Kae1_Bud32_archATPase_NBDBud32Gcp-like_domKAE1/TsaDKae1_archaea_eukKinase-like_dom_sftRNA_threonyl_synthase/kinasearch_bud32arch_KAE1gcp_kae1TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASETRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASERIO1TsaDGcp_STYKSOSIALOPTASEActin-like ATPase domainProtein kinase-like (PK-like)Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteintRNA N6-adenosine threonylcarbamoyltransferase2.3.1.234N6-L-threonylcarbamoyladenine synthaset(6)A synthaset(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1tRNA threonylcarbamoyladenosine biosynthesis protein Kae1Serine/threonine-protein kinase Bud322.7.11.1Ta0324Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.Binds 1 Fe(2+) ion per subunit.Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.In the N-terminal section; belongs to the KAE1 / TsaD family.In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein582021529Protein kinase329Kae1324Proton acceptor; for kinase activity447107111128132160173177257285335342355279212530406266687175777981981031051191251341391411521551631641671721781822012122172432462532542562582712732752802832882993063083173192001-03-011582028c2fd781989ffc4a23e6fff46d157f7eMIVLGLEGTAHTISCGIIDESRILAMESSMYRPKTGGIRPLDAAVHHSEVIDTVISRALEKAKISIHDIDLIGFSMGPGLAPSLRVTATAARTISVLTGKPIIGVNHPLGHIEIGRRVTGAIDPVMLYVSGGNTQVIAHVNGRYRVLGETLDIGIGNMIDKFAREAGIPFPGGPEIEKLAMKGTKLLDLPYSVKGMDTAFSGILTAALQYLKTGQAIEDISYSIQETAFAMLVEVLERALYVSGKDEILMAGGVALNRRLRDMVTNMAREAGIRSYLTDREYCMDNGIMIAQAALLMYKSGVRMSVEETAVNPRFRIDEVDAPWITDASRKDYGKAGAESRIEEVSFHGRPAIRKVRISKSYRNSDLDKKIRYERMRNEFTILRKLKEAGVNSPVVYDFDPFSMSITMQKIPGRMMSAELNEGRTDFLNELGIMIAKMHRAGIAHGDLTVNNIIVNDSVFIIDPSMGKVNAEIEDMAVDIYALEDSIKGLGLDSGSVIGQMLKSYRNNFNLADDVLETVSAIRRRHRYVtrue