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Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

Ta0324

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi107IronUniRule annotation1
Metal bindingi111IronUniRule annotation1
Metal bindingi128IronUniRule annotation1
Binding sitei160Threonylcarbamoyl-AMPUniRule annotation1
Binding sitei173Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation1
Binding sitei177Threonylcarbamoyl-AMPUniRule annotation1
Binding sitei257Threonylcarbamoyl-AMPUniRule annotation1
Metal bindingi285IronUniRule annotation1
Binding sitei355ATPUniRule annotation1
Active sitei447Proton acceptor; for kinase activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi335 – 342ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTACI273075:G13HZ-334-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
Gene namesi
Ordered Locus Names:Ta0324
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001024 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003036611 – 529Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinAdd BLAST529

Proteomic databases

PRIDEiQ9HLA5.

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

Protein-protein interaction databases

STRINGi273075.Ta0324.

Structurei

Secondary structure

1529
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Beta strandi9 – 21Combined sources13
Beta strandi25 – 30Combined sources6
Helixi40 – 62Combined sources23
Helixi66 – 68Combined sources3
Beta strandi71 – 75Combined sources5
Beta strandi77 – 79Combined sources3
Helixi81 – 98Combined sources18
Beta strandi103 – 105Combined sources3
Helixi107 – 119Combined sources13
Beta strandi125 – 132Combined sources8
Beta strandi134 – 139Combined sources6
Beta strandi141 – 152Combined sources12
Helixi155 – 163Combined sources9
Turni164 – 167Combined sources4
Helixi172 – 177Combined sources6
Helixi178 – 182Combined sources5
Helixi201 – 212Combined sources12
Helixi217 – 243Combined sources27
Beta strandi246 – 253Combined sources8
Helixi254 – 256Combined sources3
Helixi258 – 271Combined sources14
Beta strandi273 – 275Combined sources3
Turni280 – 283Combined sources4
Helixi288 – 299Combined sources12
Helixi306 – 308Combined sources3
Helixi317 – 319Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ENOX-ray3.02A/B1-329[»]
ProteinModelPortaliQ9HLA5.
SMRiQ9HLA5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HLA5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini329 – 529Protein kinaseUniRule annotationAdd BLAST201

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 324Kae1Add BLAST324
Regioni128 – 132Threonylcarbamoyl-AMP bindingUniRule annotation5

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
Contains 1 protein kinase domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01183. Archaea.
arCOG01185. Archaea.
COG0533. LUCA.
COG3642. LUCA.
HOGENOMiHOG000109569.
KOiK15904.
OMAiGETMDTG.

Family and domain databases

HAMAPiMF_01446. Kae1. 1 hit.
MF_01447. Kae1_Bud32_arch. 1 hit.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/TsaD.
IPR011009. Kinase-like_dom.
IPR009220. tRNA_threonyl_synthase/kinase.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HLA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVLGLEGTA HTISCGIIDE SRILAMESSM YRPKTGGIRP LDAAVHHSEV
60 70 80 90 100
IDTVISRALE KAKISIHDID LIGFSMGPGL APSLRVTATA ARTISVLTGK
110 120 130 140 150
PIIGVNHPLG HIEIGRRVTG AIDPVMLYVS GGNTQVIAHV NGRYRVLGET
160 170 180 190 200
LDIGIGNMID KFAREAGIPF PGGPEIEKLA MKGTKLLDLP YSVKGMDTAF
210 220 230 240 250
SGILTAALQY LKTGQAIEDI SYSIQETAFA MLVEVLERAL YVSGKDEILM
260 270 280 290 300
AGGVALNRRL RDMVTNMARE AGIRSYLTDR EYCMDNGIMI AQAALLMYKS
310 320 330 340 350
GVRMSVEETA VNPRFRIDEV DAPWITDASR KDYGKAGAES RIEEVSFHGR
360 370 380 390 400
PAIRKVRISK SYRNSDLDKK IRYERMRNEF TILRKLKEAG VNSPVVYDFD
410 420 430 440 450
PFSMSITMQK IPGRMMSAEL NEGRTDFLNE LGIMIAKMHR AGIAHGDLTV
460 470 480 490 500
NNIIVNDSVF IIDPSMGKVN AEIEDMAVDI YALEDSIKGL GLDSGSVIGQ
510 520
MLKSYRNNFN LADDVLETVS AIRRRHRYV
Length:529
Mass (Da):58,202
Last modified:March 1, 2001 - v1
Checksum:i9C878F334EE056D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445064 Genomic DNA. Translation: CAC11469.1.
RefSeqiWP_010900753.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC11469; CAC11469; CAC11469.
GeneIDi1455944.
KEGGitac:Ta0324.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445064 Genomic DNA. Translation: CAC11469.1.
RefSeqiWP_010900753.1. NC_002578.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ENOX-ray3.02A/B1-329[»]
ProteinModelPortaliQ9HLA5.
SMRiQ9HLA5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273075.Ta0324.

Proteomic databases

PRIDEiQ9HLA5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC11469; CAC11469; CAC11469.
GeneIDi1455944.
KEGGitac:Ta0324.

Phylogenomic databases

eggNOGiarCOG01183. Archaea.
arCOG01185. Archaea.
COG0533. LUCA.
COG3642. LUCA.
HOGENOMiHOG000109569.
KOiK15904.
OMAiGETMDTG.

Enzyme and pathway databases

BioCyciTACI273075:G13HZ-334-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9HLA5.

Family and domain databases

HAMAPiMF_01446. Kae1. 1 hit.
MF_01447. Kae1_Bud32_arch. 1 hit.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/TsaD.
IPR011009. Kinase-like_dom.
IPR009220. tRNA_threonyl_synthase/kinase.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKAE1B_THEAC
AccessioniPrimary (citable) accession number: Q9HLA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.