Q9HL75 (SPEE_THEAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable spermidine synthase
EC=2.5.1.16

Alternative name(s):
Putrescine aminopropyltransferase
Short name=PAPT
SPDSY

Gene names

Name:	speE
Ordered Locus Names:	Ta0355

Organism

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

Taxonomic identifier

273075 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor By similarity. HAMAP MF_00198
Catalytic activity	S-adenosylmethioninamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine. HAMAP MF_00198
Pathway	Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1. HAMAP MF_00198
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_00198
Sequence similarities	Belongs to the spermidine/spermine synthase family.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Spermidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	spermidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	spermidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	Probable spermidine synthase HAMAP MF_00198		PRO_0000156536
Regions
	Region	140 – 141	2	S-adenosylmethioninamine binding By similarity
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	34	1	S-adenosylmethioninamine By similarity
	Binding site	64	1	Putrescine By similarity
	Binding site	90	1	S-adenosylmethioninamine By similarity
	Binding site	109	1	S-adenosylmethioninamine By similarity
	Binding site	161	1	Putrescine By similarity
	Binding site	226	1	Putrescine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HL75 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: FADD9DF1F137E7FF	FASTA	273	31,073
	10 20 30 40 50 60 MKLIENWFSE RYSDNLQLSF RVSDQLLSIK TDYQRIDLFD TYDFGKLLAI DGTVQLTERD 70 80 90 100 110 120 EFIYHELITM VPYHLTPRPP ENALIIGGGD GGAARRLIDL GLKHIVNVEI DDQVVEVSKR 130 140 150 160 170 180 FFPSLSSAFN DSHVKLLIQD GIKYVKNTSE KFDLIIIDST DPEGPAEGLF SKDFYNDTRK 190 200 210 220 230 240 IMNEGAVIVS QSGSPFYQPK AIKLAYSGMR EVFSDVRVYT GFIPTYPSGF WSFTVASPWT 250 260 270 MKPRPVNMSG KYFNADVMEG SFRLPQFVKE LIT

References

[1]	"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL445064 Genomic DNA. Translation: CAC11499.1.
RefSeq	NP_393834.1. NC_002578.1.
3D structure databases
ProteinModelPortal	Q9HL75.
ModBase	Search...
Proteomic databases
PRIDE	Q9HL75.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1455971.
GenomeReviews	Gene locus Ta0355 in contig AL139299_GR.
KEGG	tac:Ta0355.
NMPDR	fig|273075.1.peg.356.
Phylogenomic databases
HOGENOM	HBG635113.
OMA	ELWYTEK.
PhylomeDB	Q9HL75.
ProtClustDB	PRK00811.
Enzyme and pathway databases
BioCyc	TACI273075:TA0355-MONOMER.
Family and domain databases
HAMAP	MF_00198. Spermidine_synth. [Tree]
InterPro	IPR001045. Sprmine_synthase. [Graphical view]
KO	K00797.
PANTHER	PTHR11558. Sprmine_synthase. 1 hit.
Pfam	PF01564. Spermine_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00417. SpeE. 1 hit.
PROSITE	PS01330. SPERMIDINE_SYNTHASE_1. False negative. PS51006. SPERMIDINE_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPEE_THEAC

Accession

Primary (citable) accession number: Q9HL75

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program