Q9HL74 (DHYS_THEAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable deoxyhypusine synthase
Short name=DHS
EC=2.5.1.46

Gene names

Name:	dys
Ordered Locus Names:	Ta0356

Organism

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

Taxonomic identifier

273075 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue By similarity. HAMAP MF_00153
Catalytic activity	[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine. HAMAP MF_00153
Cofactor	NAD By similarity. HAMAP MF_00153
Pathway	Protein modification; eIF5A hypusination. HAMAP MF_00153
Sequence similarities	Belongs to the deoxyhypusine synthase family.
Sequence caution	The sequence CAC11500.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Hypusine biosynthesis
Ligand	NAD
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	deoxyhypusine biosynthetic process from spermidine, using deoxyhypusine synthase Inferred from electronic annotation. Source: EC peptidyl-lysine modification to hypusine Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	deoxyhypusine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Probable deoxyhypusine synthase HAMAP MF_00153		PRO_0000134510
Sites
	Active site	284	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HL74 [UniParc]. Last modified January 31, 2002. Version 2. Checksum: DD9A8EC31A5DD904	FASTA	310	34,980
	10 20 30 40 50 60 MDRKELLSRP VRDLSITADT RLGDLMDQFS SIGGFTAAKI HEAYEIIKDM FSEDNTTFLS 70 80 90 100 110 120 FPADIISTGL RGLINEVVKR KLVDVIITTS GTLDHDIART YRNYYCGSFS YSDIELRDLG 130 140 150 160 170 180 INRLGNVLVP DESYGEIIEE KVMESLEKLY AKKKEWATVD LIHEVGLDIN SESSIIYNAA 190 200 210 220 230 240 KNNIPVFVPG ITDGSFGSQL WSFYEQHHDF KINLLEDEHR LSDIIFDAKK TGAIMIGGGI 250 260 270 280 290 300 SKHHTIWWNQ FRDGLDYAVY VTTAQEYDGS LSGAKLEEAI SWKKVRPDAR YVNVYGDATV 310 IMPVLLAPFL

References

[1]	"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL445064 Genomic DNA. Translation: CAC11500.1. Different initiation.
RefSeq	NP_393835.1. NC_002578.1.
3D structure databases
ProteinModelPortal	Q9HL74.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1455972.
GenomeReviews	Gene locus Ta0356 in contig AL139299_GR.
KEGG	tac:Ta0356.
NMPDR	fig|273075.1.peg.357.
Phylogenomic databases
HOGENOM	HBG716221.
OMA	YINTGQE.
PhylomeDB	Q9HL74.
ProtClustDB	PRK01221.
Enzyme and pathway databases
BioCyc	TACI273075:TA0356-MONOMER.
Family and domain databases
HAMAP	MF_00153. DHS. [Tree]
InterPro	IPR022899. Deoxyhypus_synthase_arc. IPR002773. Deoxyhypusine_synthase. [Graphical view]
Gene3D	G3DSA:3.40.910.10. Deoxyhypus_synth. 1 hit.
KO	K00809.
PANTHER	PTHR11703. Deoxyhypus_synth. 1 hit.
Pfam	PF01916. DS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHYS_THEAC

Accession

Primary (citable) accession number: Q9HL74

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	January 31, 2002
Last modified:	December 14, 2011
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program