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Protein

Spermidine N(1)-acetyltransferase

Gene

paiA

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the protection against polyamine toxicity by regulating their concentration. Also could be involved in the negative control of sporulation as well as production of degradative enzymes such as alpha-amylase, levansucrase and alkaline phosphatase. Catalyzes the transfer of an acetyl group from acetyl coenzyme A (AcCoA) to an acceptor substrate and release both CoA and the acetylated product. It can use a variety of substrates including spermidine, L-tryptophan, L-leucine, L-lysine, dopamine and tyramine.2 Publications

Catalytic activityi

Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine.1 Publication

Kineticsi

Kcat is 42 min(-1) for acetyltransferase activity with acetylspermidine as substrate (at pH 8.3). Kcat is 31 min(-1) for acetyltransferase activity with AcCoA as substrate (at pH 8.3).1 Publication

Manual assertion based on experiment ini

      Sites

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Binding sitei131Acetyl-CoA1 Publication1
      Binding sitei136Acetyl-CoA1 Publication1
      Binding sitei140Acetyl-CoA1 Publication1
      Sitei142May have an important role in the acetylation of the polyamineBy similarity1

      GO - Molecular functioni

      • diamine N-acetyltransferase activity Source: UniProtKB

      GO - Biological processi

      • negative regulation of sporulation Source: UniProtKB
      Complete GO annotation...

      Keywords - Molecular functioni

      Acyltransferase, Transferase

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Spermidine N(1)-acetyltransferase1 Publication (EC:2.3.1.571 Publication)
      Short name:
      SAT1 Publication
      Alternative name(s):
      GCN5-related N-acetyltransferaseCurated
      Short name:
      GNATCurated
      Protease synthase and sporulation negative regulatory protein PAI 11 Publication
      Gene namesi
      Name:paiA1 Publication
      Ordered Locus Names:Ta0374
      OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
      Taxonomic identifieri273075 [NCBI]
      Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
      Proteomesi
      • UP000001024 Componenti: Chromosome

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      ChainiPRO_00004333971 – 161Spermidine N(1)-acetyltransferaseAdd BLAST161

      Interactioni

      Subunit structurei

      Monomer or homodimer.1 Publication

      Protein-protein interaction databases

      STRINGi273075.Ta0374.

      Structurei

      Secondary structure

      1161
      Legend: HelixTurnBeta strandPDB Structure known for this area
      Show more details
      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Beta strandi3 – 7Combined sources5
      Helixi10 – 12Combined sources3
      Helixi13 – 28Combined sources16
      Turni29 – 31Combined sources3
      Helixi34 – 44Combined sources11
      Helixi47 – 58Combined sources12
      Beta strandi61 – 70Combined sources10
      Beta strandi73 – 83Combined sources11
      Beta strandi86 – 94Combined sources9
      Helixi96 – 98Combined sources3
      Helixi101 – 118Combined sources18
      Beta strandi122 – 128Combined sources7
      Helixi132 – 140Combined sources9
      Beta strandi144 – 148Combined sources5
      Beta strandi150 – 158Combined sources9

      3D structure databases

      Select the link destinations:
      PDBei
      RCSB PDBi
      PDBji
      Links Updated
      PDB entryMethodResolution (Å)ChainPositionsPDBsum
      3F0AX-ray2.50A1-159[»]
      3FIXX-ray2.30A/B/C/D1-159[»]
      3K9UX-ray2.30A/B1-159[»]
      3NE7X-ray2.30A1-159[»]
      ProteinModelPortaliQ9HL57.
      SMRiQ9HL57.
      ModBaseiSearch...
      MobiDBiSearch...

      Miscellaneous databases

      EvolutionaryTraceiQ9HL57.

      Family & Domainsi

      Domains and Repeats

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Domaini3 – 160N-acetyltransferasePROSITE-ProRule annotationAdd BLAST158

      Region

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Regioni92 – 94Acetyl-CoA-binding1 Publication3
      Regioni99 – 104Acetyl-CoA binding1 Publication6

      Sequence similaritiesi

      Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

      Phylogenomic databases

      eggNOGiarCOG00844. Archaea.
      COG0454. LUCA.
      HOGENOMiHOG000142612.
      OMAiARESWKW.

      Family and domain databases

      Gene3Di3.40.630.30. 1 hit.
      InterProiIPR016181. Acyl_CoA_acyltransferase.
      IPR000182. GNAT_dom.
      [Graphical view]
      PfamiPF00583. Acetyltransf_1. 1 hit.
      [Graphical view]
      SUPFAMiSSF55729. SSF55729. 1 hit.
      PROSITEiPS51186. GNAT. 1 hit.
      [Graphical view]

      Sequencei

      Sequence statusi: Complete.

      Q9HL57-1 [UniParc]FASTAAdd to basket

      « Hide

              10         20         30         40         50
      MSIEIRKLSI EDLETLIEVA RESWKWTYAG IYSEEYIESW IREKYSKEKL
      60 70 80 90 100
      LNEIVRSQSN LDILFLGAFA DSTLIGFIEL KIIANKAELL RLYLKPEYTH
      110 120 130 140 150
      KKIGKTLLLE AEKIMKKKGI LECRLYVHRQ NSVGFSFYYK NGFKVEDTDG
      160
      SDFIMEKKYE S
      Length:161
      Mass (Da):18,997
      Last modified:March 1, 2001 - v1
      Checksum:i934050066A9DBA05
      GO

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      AL445064 Genomic DNA. Translation: CAC11518.1.
      RefSeqiWP_010900802.1. NC_002578.1.

      Genome annotation databases

      EnsemblBacteriaiCAC11518; CAC11518; CAC11518.
      GeneIDi1455990.
      KEGGitac:Ta0374.

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      AL445064 Genomic DNA. Translation: CAC11518.1.
      RefSeqiWP_010900802.1. NC_002578.1.

      3D structure databases

      Select the link destinations:
      PDBei
      RCSB PDBi
      PDBji
      Links Updated
      PDB entryMethodResolution (Å)ChainPositionsPDBsum
      3F0AX-ray2.50A1-159[»]
      3FIXX-ray2.30A/B/C/D1-159[»]
      3K9UX-ray2.30A/B1-159[»]
      3NE7X-ray2.30A1-159[»]
      ProteinModelPortaliQ9HL57.
      SMRiQ9HL57.
      ModBaseiSearch...
      MobiDBiSearch...

      Protein-protein interaction databases

      STRINGi273075.Ta0374.

      Protocols and materials databases

      Structural Biology KnowledgebaseSearch...

      Genome annotation databases

      EnsemblBacteriaiCAC11518; CAC11518; CAC11518.
      GeneIDi1455990.
      KEGGitac:Ta0374.

      Phylogenomic databases

      eggNOGiarCOG00844. Archaea.
      COG0454. LUCA.
      HOGENOMiHOG000142612.
      OMAiARESWKW.

      Miscellaneous databases

      EvolutionaryTraceiQ9HL57.

      Family and domain databases

      Gene3Di3.40.630.30. 1 hit.
      InterProiIPR016181. Acyl_CoA_acyltransferase.
      IPR000182. GNAT_dom.
      [Graphical view]
      PfamiPF00583. Acetyltransf_1. 1 hit.
      [Graphical view]
      SUPFAMiSSF55729. SSF55729. 1 hit.
      PROSITEiPS51186. GNAT. 1 hit.
      [Graphical view]
      ProtoNetiSearch...

      Entry informationi

      Entry nameiPAIA_THEAC
      AccessioniPrimary (citable) accession number: Q9HL57
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: June 24, 2015
      Last sequence update: March 1, 2001
      Last modified: November 2, 2016
      This is version 73 of the entry and version 1 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programProkaryotic Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      3D-structure, Complete proteome, Reference proteome

      Documents

      1. PDB cross-references
        Index of Protein Data Bank (PDB) cross-references
      2. SIMILARITY comments
        Index of protein domains and families

      Similar proteinsi

      Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
      100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
      90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
      50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.