Q9HKM5 (HEM3_THEAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable porphobilinogen deaminase
Short name=PBG
EC=2.5.1.61

Alternative name(s):
Hydroxymethylbilane synthase
Short name=HMBS
Pre-uroporphyrinogen synthase

Gene names

Name:	hemC
Ordered Locus Names:	Ta0572

Organism

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

Taxonomic identifier

273075 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps. HAMAP MF_00260
Catalytic activity	4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3. HAMAP MF_00260
Cofactor	Binds 1 dipyrromethane group covalently By similarity. HAMAP MF_00260
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4. HAMAP MF_00260
Miscellaneous	The porphobilinogen subunits are added to the dipyrromethan group. HAMAP MF_00260
Sequence similarities	Belongs to the HMBS family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	peptidyl-pyrromethane cofactor linkage Inferred from electronic annotation. Source: InterPro porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	hydroxymethylbilane synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Probable porphobilinogen deaminase HAMAP MF_00260		PRO_0000143032
Amino acid modifications
	Modified residue	234	1	S-(dipyrrolylmethanemethyl)cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HKM5 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: E25EE90441ACBF3F	FASTA	295	32,775
	10 20 30 40 50 60 MITIRIGTRP SRLAVRQAEI VASAIRHAGY EVEIVKYRSE GDSDLKSPLY SIGKTGVFVE 70 80 90 100 110 120 RLNSLILSGD IDAAVHSAKD IPYEIDRRLR ISAVMPRGRF EDALVSENPL SKLPPGSVIG 130 140 150 160 170 180 TSSLRRRYQI LYQRKDVKVS NIRGNVDTRI EKMRSEGMAG IVMALAAIDR LELNLRYWPF 190 200 210 220 230 240 DPEKFVPAPN QGIIAVVSED GSKASEVLSS INDADTYDDM MAERSITQGL KLGCSTPVGI 250 260 270 280 290 LSRHTGKGMR ILAQFFAMDG SDMMMFDQHV DGLDDVDGIV SYIRENLPEE YGYKL

References

[1]	"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL445064 Genomic DNA. Translation: CAC11712.1.
RefSeq	NP_394046.1. NC_002578.1.
3D structure databases
ProteinModelPortal	Q9HKM5.
ModBase	Search...
Proteomic databases
PRIDE	Q9HKM5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1456162.
GenomeReviews	Gene locus Ta0572 in contig AL139299_GR.
KEGG	tac:Ta0572.
NMPDR	fig|273075.1.peg.568.
Phylogenomic databases
HOGENOM	HBG624842.
OMA	LRDMGAF.
PhylomeDB	Q9HKM5.
ProtClustDB	PRK00072.
Enzyme and pathway databases
BioCyc	TACI273075:TA0572-MONOMER.
Family and domain databases
HAMAP	MF_00260. Porphobil_deam. [Tree]
InterPro	IPR000860. 4pyrrol_synth_OHMeBilane_synth. IPR022419. Porphobilin_deaminase_cofac_BS. IPR022417. Porphobilin_deaminase_N. IPR022418. Porphobilinogen_deaminase_C. [Graphical view]
Gene3D	G3DSA:3.30.160.40. Porphobil_deam. 1 hit.
KO	K01749.
PANTHER	PTHR11557. Porphobil_deam. 1 hit.
Pfam	PF01379. Porphobil_deam. 1 hit. [Graphical view]
PIRSF	PIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTS	PR00151. PORPHBDMNASE.
SUPFAM	SSF54782. Porphobil_deam. 1 hit.
TIGRFAMs	TIGR00212. HemC. 1 hit.
PROSITE	PS00533. PORPHOBILINOGEN_DEAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM3_THEAC

Accession

Primary (citable) accession number: Q9HKM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program