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Q9HK31 (SYL_THEAC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Ta0777
OrganismThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) [Reference proteome] [HAMAP]
Taxonomic identifier273075 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma

Protein attributes

Sequence length910 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 910910Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152148

Regions

Motif50 – 6011"HIGH" region HAMAP-Rule MF_00049
Motif611 – 6155"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6141ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9HK31 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 17FA01BB946DF0BE

FASTA910105,028
        10         20         30         40         50         60 
MDDRGRRCCT HSGVIALDIE AKWQNAWDRD GIFVPKMDGR KKFMITVPWP YTNGSLHVGH 

        70         80         90        100        110        120 
GRTYTLGDII ARYKRSRNYN VLFPMGFHQS GTPILAFSER IRAGDRSTID LYTSYLKEYG 

       130        140        150        160        170        180 
EKDIDALIES FKDPKNIADY FSNAIINDFK HLGYSIDWTR RFTSADEFYQ KFVQWQFRRL 

       190        200        210        220        230        240 
NEKGLVKQGR YPILYSLEDD NAVGEDDIKD GDTDKVTIEE YTAIFFRGKS FDLIAASLRP 

       250        260        270        280        290        300 
ETIYGITNIW VNPDVKYVKV KISGRMAVVS EECSTKLKFQ GNEIEVAGEA SVQEIQKQTY 

       310        320        330        340        350        360 
TTPAGKEVKV YQADFVDPDN GTGIVYSVPS HSVYDYVYYR KKRGKDFPVI IEAPMKMKDI 

       370        380        390        400        410        420 
ESKYDLETEE GREEATKDLY RNEFYYGKLV DSGPYTGMTV REAREAVKRD LISSGNAFTF 

       430        440        450        460        470        480 
YETSRHAVTR SGSKVIVAVL PDQWFLDYSQ PWLKDLGHTM INTMTMHPEV YRNVMNDAID 

       490        500        510        520        530        540 
WLKERPCARR RGLGTRLPFD DRWVIESLSD STIYPAVYTN SIPLRSLYET GKLDDDAITR 

       550        560        570        580        590        600 
IFMNGEPKNE DESEAKRQFE YWYPVDIRLT AIPHISNHLS FYVLNHAAIF PKEKWPAGLI 

       610        620        630        640        650        660 
ISGLVVSNGA KISKSKGNVV SLLEIAKKYS ADIYRLYVAV QADISSTMDW NETDLASITR 

       670        680        690        700        710        720 
RFNEFKDLMA GFKQDTSDLT FEEAWFVARF SVRLRQFMES MDRYQIRDAY INIFYGVLND 

       730        740        750        760        770        780 
LRYLSSRGGD VNRALTPVIA DWLRALMPVI PHHAEEYWHS YVSDTYVSVD PFDENFQDRY 

       790        800        810        820        830        840 
ERTVRRFGMT CDQMYSAMDY VEKVLQDVKN IMQVTGIEPK SVEITVANAD VVRAAQEFLN 

       850        860        870        880        890        900 
NSVSGQSKKY MQYLAKRRKD IMIYGFDEYD VLQRNQVYLS KQIGCPVRIE RGDVINGKIA 

       910 
LPGKPVIYIS 

« Hide

References

[1]"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL445065 Genomic DNA. Translation: CAC11908.1.
RefSeqNP_394239.1. NC_002578.1.

3D structure databases

ProteinModelPortalQ9HK31.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273075.Ta0777.

Proteomic databases

PRIDEQ9HK31.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC11908; CAC11908; CAC11908.
GeneID1456331.
KEGGtac:Ta0777.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000216620.
KOK01869.
OMAMRINDAT.
ProtClustDBPRK12300.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_A. Leu_tRNA_synth_A.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00395. leuS_arch. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_THEAC
AccessionPrimary (citable) accession number: Q9HK31
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries