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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei614 – 6141ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Ta0777
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
ProteomesiUP000001024 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 910910Leucine--tRNA ligasePRO_0000152148Add
BLAST

Proteomic databases

PRIDEiQ9HK31.

Interactioni

Protein-protein interaction databases

STRINGi273075.Ta0777.

Structurei

3D structure databases

ProteinModelPortaliQ9HK31.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 6011"HIGH" regionAdd
BLAST
Motifi611 – 6155"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiYSADIYR.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HK31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDRGRRCCT HSGVIALDIE AKWQNAWDRD GIFVPKMDGR KKFMITVPWP
60 70 80 90 100
YTNGSLHVGH GRTYTLGDII ARYKRSRNYN VLFPMGFHQS GTPILAFSER
110 120 130 140 150
IRAGDRSTID LYTSYLKEYG EKDIDALIES FKDPKNIADY FSNAIINDFK
160 170 180 190 200
HLGYSIDWTR RFTSADEFYQ KFVQWQFRRL NEKGLVKQGR YPILYSLEDD
210 220 230 240 250
NAVGEDDIKD GDTDKVTIEE YTAIFFRGKS FDLIAASLRP ETIYGITNIW
260 270 280 290 300
VNPDVKYVKV KISGRMAVVS EECSTKLKFQ GNEIEVAGEA SVQEIQKQTY
310 320 330 340 350
TTPAGKEVKV YQADFVDPDN GTGIVYSVPS HSVYDYVYYR KKRGKDFPVI
360 370 380 390 400
IEAPMKMKDI ESKYDLETEE GREEATKDLY RNEFYYGKLV DSGPYTGMTV
410 420 430 440 450
REAREAVKRD LISSGNAFTF YETSRHAVTR SGSKVIVAVL PDQWFLDYSQ
460 470 480 490 500
PWLKDLGHTM INTMTMHPEV YRNVMNDAID WLKERPCARR RGLGTRLPFD
510 520 530 540 550
DRWVIESLSD STIYPAVYTN SIPLRSLYET GKLDDDAITR IFMNGEPKNE
560 570 580 590 600
DESEAKRQFE YWYPVDIRLT AIPHISNHLS FYVLNHAAIF PKEKWPAGLI
610 620 630 640 650
ISGLVVSNGA KISKSKGNVV SLLEIAKKYS ADIYRLYVAV QADISSTMDW
660 670 680 690 700
NETDLASITR RFNEFKDLMA GFKQDTSDLT FEEAWFVARF SVRLRQFMES
710 720 730 740 750
MDRYQIRDAY INIFYGVLND LRYLSSRGGD VNRALTPVIA DWLRALMPVI
760 770 780 790 800
PHHAEEYWHS YVSDTYVSVD PFDENFQDRY ERTVRRFGMT CDQMYSAMDY
810 820 830 840 850
VEKVLQDVKN IMQVTGIEPK SVEITVANAD VVRAAQEFLN NSVSGQSKKY
860 870 880 890 900
MQYLAKRRKD IMIYGFDEYD VLQRNQVYLS KQIGCPVRIE RGDVINGKIA
910
LPGKPVIYIS
Length:910
Mass (Da):105,028
Last modified:March 1, 2001 - v1
Checksum:i17FA01BB946DF0BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445065 Genomic DNA. Translation: CAC11908.1.
RefSeqiNP_394239.1. NC_002578.1.
WP_010901190.1. NC_002578.1.

Genome annotation databases

GeneIDi1456331.
KEGGitac:Ta0777.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445065 Genomic DNA. Translation: CAC11908.1.
RefSeqiNP_394239.1. NC_002578.1.
WP_010901190.1. NC_002578.1.

3D structure databases

ProteinModelPortaliQ9HK31.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273075.Ta0777.

Proteomic databases

PRIDEiQ9HK31.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1456331.
KEGGitac:Ta0777.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000216620.
KOiK01869.
OMAiYSADIYR.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_A. Leu_tRNA_synth_A.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR020791. Leu-tRNA-lgase_arc.
IPR004493. Leu-tRNA-synth_Ia_arc/euk.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00395. leuS_arch. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
    Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
    Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Entry informationi

Entry nameiSYL_THEAC
AccessioniPrimary (citable) accession number: Q9HK31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: March 1, 2001
Last modified: April 1, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.