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Q9HJT4

- SYI_THEAC

UniProt

Q9HJT4 - SYI_THEAC

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 2 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei594 – 5941ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Ta0879
    OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
    Taxonomic identifieri273075 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
    ProteomesiUP000001024: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10261026Isoleucine--tRNA ligasePRO_0000098595Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi273075.Ta0879.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HJT4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi51 – 6111"HIGH" regionAdd
    BLAST
    Motifi591 – 5955"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRVEHMVE.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HJT4-1 [UniParc]FASTAAdd to Basket

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    MQSLRFRQID PGMTLREIDS EILKYWKDKN ILEKILSKGG SKKFVFLEGP     50
    PTANGRPHIG HAMTRTIKDI VLRYNTMTDH KIYRRVGGWD CHGLPVELEA 100
    EKHFGFHTKS EIVNFGVEKF NQYCRESIFR YIDEWKQVDD LIGFSIDHNG 150
    DYITLRNDYM ESEWFALKTM YNSGLLYKDY TVVPYCPRCE TSLSSHEVAQ 200
    GYKDVKDPSV YVRFKSADEE NTYFVAWTTT PWTLPSNEFL VVNPDMEYSL 250
    VEAQGSRYYV ASSRAGYIFK EYREIRRMHG RDLVGKRYLQ LMPFLDPPSG 300
    SLKVVAGSFV TSEDGSGIVH AAPAFGADDY QIGKEEGVEI LNPVDKNGRF 350
    ADPRIPWNGK FVRDANEDII VYLKKNQMLL KSEKYEHSYP FCYRCDTPLL 400
    YYPLDAWFIA VSRIRDKLVE YNERINWKPD YLKHGRFGNF LGEAKDWNLS 450
    RDRFWGTPLP AWRCKNGHLV FVGSRKEIED LGGKVPEDLH RPYIDEVRFK 500
    CPTCGEEMSR EPYVIDTWFD SGSATYAASH YPFEKNFDPE TDVPVSFITE 550
    AIDQTRGWFY VLHVIATIMF NKNAYESALS INFILDAQGR KMSKSKGNSV 600
    YALDFLNEVP PDSLRLFFLY GAPWKSKNLD KKVIDEVSRK TLMTVLNVYS 650
    FFAYNANIDN FQWNGLQLSG NALDRYMVSK VNSFVRSSRD AYESLDFHEV 700
    VRASMEFVDD LSNFYLRLSR RRFWAEGFDD DKLSAYSTLY YALKAFSEVM 750
    APITPFFSDF IYLNLGGDKE SVHLEAFPEF DSTLMDEKLE SEMDRAYSVI 800
    ETVRRLRQEN SIKGRQPLRE ILIAGDMEES IIDVVKSELN AKDIKLIERD 850
    QEPIRLSADL RMDRAAPVLR SRVNAVRHKI RSMDGLEVQR QISEKGFVEI 900
    DGVRLDPDMV EISRVPDPNY AYSQTEKYGI DVFINKNIDR DGYLEGLARE 950
    LVRRIQVMRK EMNLNYTDRI ITHLDLSDDF LEALNKHAEY IKNETQSDSI 1000
    ITDKVEGMKL WEINGEPVRI KIDLAR 1026
    Length:1,026
    Mass (Da):119,169
    Last modified:December 20, 2005 - v2
    Checksum:i0FE6AA2C39E9B50F
    GO

    Sequence cautioni

    The sequence CAC12008.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL445065 Genomic DNA. Translation: CAC12008.1. Different initiation.
    RefSeqiNP_394338.1. NC_002578.1.

    Genome annotation databases

    EnsemblBacteriaiCAC12008; CAC12008; CAC12008.
    GeneIDi1456418.
    KEGGitac:Ta0879.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL445065 Genomic DNA. Translation: CAC12008.1 . Different initiation.
    RefSeqi NP_394338.1. NC_002578.1.

    3D structure databases

    ProteinModelPortali Q9HJT4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273075.Ta0879.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC12008 ; CAC12008 ; CAC12008 .
    GeneIDi 1456418.
    KEGGi tac:Ta0879.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RVEHMVE.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
      Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
      Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

    Entry informationi

    Entry nameiSYI_THEAC
    AccessioniPrimary (citable) accession number: Q9HJT4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 76 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3