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Q9HJT4

- SYI_THEAC

UniProt

Q9HJT4 - SYI_THEAC

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei594 – 5941ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Ta0879
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
ProteomesiUP000001024: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10261026Isoleucine--tRNA ligasePRO_0000098595Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi273075.Ta0879.

Structurei

3D structure databases

ProteinModelPortaliQ9HJT4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi51 – 6111"HIGH" regionAdd
BLAST
Motifi591 – 5955"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HJT4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQSLRFRQID PGMTLREIDS EILKYWKDKN ILEKILSKGG SKKFVFLEGP
60 70 80 90 100
PTANGRPHIG HAMTRTIKDI VLRYNTMTDH KIYRRVGGWD CHGLPVELEA
110 120 130 140 150
EKHFGFHTKS EIVNFGVEKF NQYCRESIFR YIDEWKQVDD LIGFSIDHNG
160 170 180 190 200
DYITLRNDYM ESEWFALKTM YNSGLLYKDY TVVPYCPRCE TSLSSHEVAQ
210 220 230 240 250
GYKDVKDPSV YVRFKSADEE NTYFVAWTTT PWTLPSNEFL VVNPDMEYSL
260 270 280 290 300
VEAQGSRYYV ASSRAGYIFK EYREIRRMHG RDLVGKRYLQ LMPFLDPPSG
310 320 330 340 350
SLKVVAGSFV TSEDGSGIVH AAPAFGADDY QIGKEEGVEI LNPVDKNGRF
360 370 380 390 400
ADPRIPWNGK FVRDANEDII VYLKKNQMLL KSEKYEHSYP FCYRCDTPLL
410 420 430 440 450
YYPLDAWFIA VSRIRDKLVE YNERINWKPD YLKHGRFGNF LGEAKDWNLS
460 470 480 490 500
RDRFWGTPLP AWRCKNGHLV FVGSRKEIED LGGKVPEDLH RPYIDEVRFK
510 520 530 540 550
CPTCGEEMSR EPYVIDTWFD SGSATYAASH YPFEKNFDPE TDVPVSFITE
560 570 580 590 600
AIDQTRGWFY VLHVIATIMF NKNAYESALS INFILDAQGR KMSKSKGNSV
610 620 630 640 650
YALDFLNEVP PDSLRLFFLY GAPWKSKNLD KKVIDEVSRK TLMTVLNVYS
660 670 680 690 700
FFAYNANIDN FQWNGLQLSG NALDRYMVSK VNSFVRSSRD AYESLDFHEV
710 720 730 740 750
VRASMEFVDD LSNFYLRLSR RRFWAEGFDD DKLSAYSTLY YALKAFSEVM
760 770 780 790 800
APITPFFSDF IYLNLGGDKE SVHLEAFPEF DSTLMDEKLE SEMDRAYSVI
810 820 830 840 850
ETVRRLRQEN SIKGRQPLRE ILIAGDMEES IIDVVKSELN AKDIKLIERD
860 870 880 890 900
QEPIRLSADL RMDRAAPVLR SRVNAVRHKI RSMDGLEVQR QISEKGFVEI
910 920 930 940 950
DGVRLDPDMV EISRVPDPNY AYSQTEKYGI DVFINKNIDR DGYLEGLARE
960 970 980 990 1000
LVRRIQVMRK EMNLNYTDRI ITHLDLSDDF LEALNKHAEY IKNETQSDSI
1010 1020
ITDKVEGMKL WEINGEPVRI KIDLAR
Length:1,026
Mass (Da):119,169
Last modified:December 20, 2005 - v2
Checksum:i0FE6AA2C39E9B50F
GO

Sequence cautioni

The sequence CAC12008.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445065 Genomic DNA. Translation: CAC12008.1. Different initiation.
RefSeqiNP_394338.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC12008; CAC12008; CAC12008.
GeneIDi1456418.
KEGGitac:Ta0879.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445065 Genomic DNA. Translation: CAC12008.1 . Different initiation.
RefSeqi NP_394338.1. NC_002578.1.

3D structure databases

ProteinModelPortali Q9HJT4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273075.Ta0879.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC12008 ; CAC12008 ; CAC12008 .
GeneIDi 1456418.
KEGGi tac:Ta0879.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
    Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
    Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Entry informationi

Entry nameiSYI_THEAC
AccessioniPrimary (citable) accession number: Q9HJT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 26, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3