Q9HJR8 (NADE_THEAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Ta0899

Organism

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

Taxonomic identifier

273075 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152237
Regions
	Nucleotide binding	27 – 34	8	ATP By similarity
Sites
	Active site	29	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HJR8 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 6A9BB2DA111D6C90	FASTA	241	27,367
	10 20 30 40 50 60 MPDYMQEIER ISEFLRKVLQ GKNAVIGISG GIDSSVTLAL LTRSIPKERI IPVFMPDRNT 70 80 90 100 110 120 QQADYDDVQK LTQKLGLTYR EVRIDPMVDS FVSTLAATDR AAIGNIKSRT RMIILYYFAN 130 140 150 160 170 180 TNGGMVVGTT NRTELLLGYF TKYGDGGCDV EPIEHLYKRD VYELAKILDI PESIMKKKPT 190 200 210 220 230 240 AGLWAGQTDE DEIGMSYAQM DEILSDVFDK GTYNDKPEYK KIMEMHSLSD HKRNLPYSLK K

References

[1]	"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL445065 Genomic DNA. Translation: CAC12028.1.
RefSeq	NP_394358.1. NC_002578.1.
3D structure databases
ProteinModelPortal	Q9HJR8.
ModBase	Search...
Proteomic databases
PRIDE	Q9HJR8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1456435.
GenomeReviews	Gene locus Ta0899 in contig AL139299_GR.
KEGG	tac:Ta0899.
NMPDR	fig|273075.1.peg.880.
Phylogenomic databases
HOGENOM	HBG351567.
OMA	YDISARD.
PhylomeDB	Q9HJR8.
ProtClustDB	PRK13980.
Enzyme and pathway databases
BioCyc	TACI273075:TA0899-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_THEAC

Accession

Primary (citable) accession number: Q9HJR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program