Reviewed, UniProtKB/Swiss-Prot Q9HJM3 (GUAAA_THEAC)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit A
    EC=6.3.5.2
Alternative name(s):
    Glutamine amidotransferase

Gene names

Name:	guaAA
Ordered Locus Names:	Ta0944

Organism

Thermoplasma acidophilum [Complete proteome] [HAMAP]

Taxonomic identifier

2303 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_01510
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential. HAMAP MF_01510
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 200

200

GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510

PRO_0000140235

Regions

Domain

3 – 193

191

Glutamine amidotransferase type-1

Sites

Active site

80

1

Nucleophile By similarity

Active site

167

1

By similarity

Active site

169

1

By similarity

Secondary structure

1

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200

Helix Strand Turn

Details...

Beta strand

3 – 10

8

Helix

16 – 23

8

Beta strand

30 – 32

3

Helix

37 – 40

4

Beta strand

44 – 48

5

Helix

55 – 57

3

Helix

59 – 61

3

Helix

62 – 71

10

Beta strand

76 – 79

4

Helix

81 – 89

9

Beta strand

93 – 110

18

Helix

114 – 116

3

Beta strand

121 – 135

15

Beta strand

140 – 145

6

Beta strand

152 – 166

15

Helix

176 – 195

20

Sequences

	Sequence		Length	Mass (Da)
	Q9HJM3-1 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: 747E9F28574E7C6A	FASTA	200	22,471
	10 20 30 40 50 60 MLKIYVVDNG GQWTHREWRV LRELGVDTKI VPNDIDSSEL DGLDGLVLSG GAPNIDEELD 70 80 90 100 110 120 KLGSVGKYID DHNYPILGIC VGAQFIALHF GASVVKAKHP EFGKTKVSVM HSENIFGGLP 130 140 150 160 170 180 SEITVWENHN DEIINLPDDF TLAASSATCQ VQGFYHKTRP IYATQFHPEV EHTQYGRDIF 190 200 RNFIGICASY REIQKENFQH

References

[1]

"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / AMRC-C165 / DSM 1728 / IFO 15155 / JCM 9062.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL445066 Genomic DNA. Translation: CAC12073.1. Different initiation.

RefSeq

NP_394403.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2A9V	X-ray	2.24	A/B/C/D	1-200	[»]

ModBase

Search...

Genome annotation databases

GeneID

1457057.

NMPDR

fig|273075.1.peg.925.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HBG292341.

OMA

GQYVHRI.

Enzyme and pathway databases

BioCyc

TACI273075:TA0944-MONOMER.

BRENDA

6.3.5.2. 435.

Family and domain databases

HAMAP

MF_01510. GMP_synthase_A.
[Tree]

InterPro

IPR006220. Anth_synthII.
IPR017926. GATASE_1.
IPR000991. GATase_class1_C.
IPR004739. GMP_synth_N.
[Graphical view]

Pfam

PF00117. GATase. 1 hit.
[Graphical view]

PRINTS

PR00097. ANTSNTHASEII.

TIGRFAMs

TIGR00888. guaA_Nterm. 1 hit.

PROSITE

PS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GUAAA_THEAC

Accession

Primary (citable) accession number: Q9HJM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2004
Last modified:	February 9, 2010
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)