Q9HJL5 (MDH_THEAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	Ta0952

Organism

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) [Reference proteome] [HAMAP]

Taxonomic identifier

273075 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma › Thermoplasma acidophilum

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Malate dehydrogenase HAMAP-Rule MF_00487		PRO_0000113495
Regions
	Nucleotide binding	10 – 15	6	NAD By similarity
	Nucleotide binding	124 – 126	3	NAD By similarity
Sites
	Active site	181	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	88	1	Substrate By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	101	1	NAD By similarity
	Binding site	126	1	Substrate By similarity
	Binding site	157	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HJL5 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: D6126D95B7EB1B31	FASTA	325	35,524
	10 20 30 40 50 60 MARKKISVIG AGNVGATVAQ FLAAKQLGDV YLFDVVDGIP EGKALDIQEG APHWRYDLDV 70 80 90 100 110 120 VGFSTSDETK YKNMEGSDVI VVTAGLARKP GMSRDDLFDK NVEIISDVSR NIKKYSPDSI 130 140 150 160 170 180 IVVVSNPADI MAYALQKFTG IDPSKIMGLG GSLDSSRFRT FLAKELNVSV EDVNAFVIGG 190 200 210 220 230 240 HGDDMVPFIR YSSVAGIPIE NLLSKEKIDE IVKRTRFGGG EIVNYLKTGS AFYAPGISIT 250 260 270 280 290 300 AMVESVIMDK KRVIPCAAYI TGKHADHYGI RDKFIGVPIK IGEKGVEQIY DIDFKPDELE 310 320 LWKKSVASVE ASSKKVDEWI AKHAH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the gene for the L-malate dehydrogenase from the thermoacidophilic archaebacterium Thermoplasma acidophilum." Martinez D., Trejo F., Gelpi J.L., Busquets M., Cortes A. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF199510 Genomic DNA. Translation: AAG28562.1. AL445066 Genomic DNA. Translation: CAC12081.1.
RefSeq	NP_394412.1. NC_002578.1.
3D structure databases
ProteinModelPortal	Q9HJL5.
SMR	Q9HJL5. Positions 5-312.
ModBase	Search...
Protein-protein interaction databases
STRING	273075.Ta0952.
Proteomic databases
PRIDE	Q9HJL5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC12081; CAC12081; CAC12081.
GeneID	1456484.
KEGG	tac:Ta0952.
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213794.
KO	K00024.
OMA	DAMTYVM.
ProtClustDB	PRK06223.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_THEAC

Accession

Primary (citable) accession number: Q9HJL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 1, 2001
Last modified:	May 29, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program