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Protein

Malate dehydrogenase

Gene

mdh

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADBy similarity
Binding sitei88 – 881SubstrateBy similarity
Binding sitei94 – 941SubstrateBy similarity
Binding sitei101 – 1011NADBy similarity
Binding sitei126 – 1261SubstrateBy similarity
Binding sitei157 – 1571SubstrateBy similarity
Active sitei181 – 1811Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156NADBy similarity
Nucleotide bindingi124 – 1263NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
Ordered Locus Names:Ta0952
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
ProteomesiUP000001024: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 325325Malate dehydrogenasePRO_0000113495Add
BLAST

Proteomic databases

PRIDEiQ9HJL5.

Interactioni

Protein-protein interaction databases

STRINGi273075.Ta0952.

Structurei

3D structure databases

ProteinModelPortaliQ9HJL5.
SMRiQ9HJL5. Positions 5-312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.Curated

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiQKVVGMA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HJL5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARKKISVIG AGNVGATVAQ FLAAKQLGDV YLFDVVDGIP EGKALDIQEG
60 70 80 90 100
APHWRYDLDV VGFSTSDETK YKNMEGSDVI VVTAGLARKP GMSRDDLFDK
110 120 130 140 150
NVEIISDVSR NIKKYSPDSI IVVVSNPADI MAYALQKFTG IDPSKIMGLG
160 170 180 190 200
GSLDSSRFRT FLAKELNVSV EDVNAFVIGG HGDDMVPFIR YSSVAGIPIE
210 220 230 240 250
NLLSKEKIDE IVKRTRFGGG EIVNYLKTGS AFYAPGISIT AMVESVIMDK
260 270 280 290 300
KRVIPCAAYI TGKHADHYGI RDKFIGVPIK IGEKGVEQIY DIDFKPDELE
310 320
LWKKSVASVE ASSKKVDEWI AKHAH
Length:325
Mass (Da):35,524
Last modified:March 1, 2001 - v1
Checksum:iD6126D95B7EB1B31
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF199510 Genomic DNA. Translation: AAG28562.1.
AL445066 Genomic DNA. Translation: CAC12081.1.
RefSeqiNP_394412.1. NC_002578.1.
WP_010901363.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC12081; CAC12081; CAC12081.
GeneIDi1456484.
KEGGitac:Ta0952.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF199510 Genomic DNA. Translation: AAG28562.1.
AL445066 Genomic DNA. Translation: CAC12081.1.
RefSeqiNP_394412.1. NC_002578.1.
WP_010901363.1. NC_002578.1.

3D structure databases

ProteinModelPortaliQ9HJL5.
SMRiQ9HJL5. Positions 5-312.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273075.Ta0952.

Proteomic databases

PRIDEiQ9HJL5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC12081; CAC12081; CAC12081.
GeneIDi1456484.
KEGGitac:Ta0952.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiQKVVGMA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of the gene for the L-malate dehydrogenase from the thermoacidophilic archaebacterium Thermoplasma acidophilum."
    Martinez D., Trejo F., Gelpi J.L., Busquets M., Cortes A.
    Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
    Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
    Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Entry informationi

Entry nameiMDH_THEAC
AccessioniPrimary (citable) accession number: Q9HJL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: March 1, 2001
Last modified: January 7, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.