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Protein

Riboflavin kinase

Gene

ribK

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN).By similarity

Catalytic activityi

CTP + riboflavin = CDP + FMN.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: FMN biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes FMN from riboflavin (CTP route).
Proteins known to be involved in this subpathway in this organism are:
  1. Riboflavin kinase (ribK)
This subpathway is part of the pathway FMN biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes FMN from riboflavin (CTP route), the pathway FMN biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi131MagnesiumBy similarity1
Metal bindingi133MagnesiumBy similarity1
Binding sitei188FMNBy similarity1
Binding sitei195FMNBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi102 – 107CDPBy similarity6
Nucleotide bindingi200 – 203CDPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

Flavoprotein, FMN, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00276; UER00929.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin kinase (EC:2.7.1.161)
Short name:
RFK
Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase
Gene namesi
Name:ribK
Ordered Locus Names:Ta1064
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001024 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003221061 – 220Riboflavin kinaseAdd BLAST220

Proteomic databases

PRIDEiQ9HJA6.

Interactioni

Protein-protein interaction databases

STRINGi273075.Ta1064.

Structurei

Secondary structure

1220
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 17Combined sources12
Turni18 – 20Combined sources3
Beta strandi21 – 25Combined sources5
Helixi28 – 35Combined sources8
Helixi39 – 51Combined sources13
Beta strandi54 – 60Combined sources7
Beta strandi63 – 68Combined sources6
Helixi70 – 86Combined sources17
Beta strandi93 – 99Combined sources7
Helixi116 – 122Combined sources7
Beta strandi133 – 136Combined sources4
Helixi138 – 140Combined sources3
Helixi141 – 149Combined sources9
Beta strandi153 – 155Combined sources3
Beta strandi168 – 175Combined sources8
Beta strandi178 – 187Combined sources10
Beta strandi192 – 197Combined sources6
Beta strandi213 – 218Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CTAX-ray2.20A2-220[»]
ProteinModelPortaliQ9HJA6.
SMRiQ9HJA6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HJA6.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 92H-T-H motif-likeAdd BLAST92
Regioni93 – 220Riboflavin kinaseAdd BLAST128

Sequence similaritiesi

Belongs to the archaeal riboflavin kinase family.Curated

Phylogenomic databases

eggNOGiarCOG01904. Archaea.
COG1339. LUCA.
HOGENOMiHOG000015512.
KOiK07732.
OMAiRHYISLP.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.40.30.30. 1 hit.
InterProiIPR012318. HTH_CRP.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023602. Riboflavin_kinase_CTP-dep.
IPR023465. Riboflavin_kinase_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01982. CTP-dep_RFKase. 1 hit.
PF13545. HTH_Crp_2. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF82114. SSF82114. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HJA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METDDQYYRA IKKIKEAAEA SNRAYLTSSK LADMLGISQQ SASRIIIDLE
60 70 80 90 100
KNGYITRTVT KRGQILNITE KGLDVLYTEF ADLSRILAIK NNVVITGTVT
110 120 130 140 150
SGMGEGRYYV ARKQYIIQFQ EKLGIIPYLG TLNIKVDQAS LPELRKIRGF
160 170 180 190 200
RGIHIEGFKT EDRTFGSVKA FPAKIQNIPC FVIMPERTVY TDVIEIISDK
210 220
YLREEINLHD GDRVSVEVYT
Length:220
Mass (Da):24,997
Last modified:March 1, 2001 - v1
Checksum:iBB0F694447629612
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445066 Genomic DNA. Translation: CAC12192.1.
RefSeqiWP_010901475.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC12192; CAC12192; CAC12192.
GeneIDi1456578.
KEGGitac:Ta1064.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445066 Genomic DNA. Translation: CAC12192.1.
RefSeqiWP_010901475.1. NC_002578.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CTAX-ray2.20A2-220[»]
ProteinModelPortaliQ9HJA6.
SMRiQ9HJA6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273075.Ta1064.

Proteomic databases

PRIDEiQ9HJA6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC12192; CAC12192; CAC12192.
GeneIDi1456578.
KEGGitac:Ta1064.

Phylogenomic databases

eggNOGiarCOG01904. Archaea.
COG1339. LUCA.
HOGENOMiHOG000015512.
KOiK07732.
OMAiRHYISLP.

Enzyme and pathway databases

UniPathwayiUPA00276; UER00929.

Miscellaneous databases

EvolutionaryTraceiQ9HJA6.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.40.30.30. 1 hit.
InterProiIPR012318. HTH_CRP.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023602. Riboflavin_kinase_CTP-dep.
IPR023465. Riboflavin_kinase_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01982. CTP-dep_RFKase. 1 hit.
PF13545. HTH_Crp_2. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF82114. SSF82114. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRIFK_THEAC
AccessioniPrimary (citable) accession number: Q9HJA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.