Reviewed, UniProtKB/Swiss-Prot Q9HJA6 (RIFK_THEAC)

Names and origin

Protein names

Recommended name:
    Riboflavin kinase
      Short name=RFK
    EC=2.7.1.161
Alternative name(s):
    CTP:riboflavin 5'-phosphotransferase
    CTP-dependent riboflavin kinase
    Flavokinase

Gene names

Name:	rfk
Ordered Locus Names:	Ta1064

Organism

Thermoplasma acidophilum [Complete proteome] [HAMAP]

Taxonomic identifier

2303 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity.
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	FMN biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP riboflavin kinase activity Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 220

220

Riboflavin kinase HAMAP MF_01285

PRO_0000322106

Regions

Nucleotide binding

102 – 107

6

CDP By similarity

Nucleotide binding

200 – 203

4

CDP By similarity

Region

1 – 92

92

H-T-H motif-like HAMAP MF_01285

Region

93 – 220

128

Riboflavin kinase HAMAP MF_01285

Sites

Metal binding

131

1

Magnesium By similarity

Metal binding

133

1

Magnesium By similarity

Binding site

188

1

FMN By similarity

Binding site

195

1

FMN By similarity

Secondary structure

1

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220

Helix Strand Turn

Details...

Helix

6 – 17

12

Turn

18 – 20

3

Beta strand

21 – 25

5

Helix

28 – 35

8

Helix

39 – 51

13

Beta strand

54 – 60

7

Beta strand

63 – 68

6

Helix

70 – 86

17

Beta strand

93 – 99

7

Helix

116 – 122

7

Beta strand

133 – 136

4

Helix

138 – 140

3

Helix

141 – 149

9

Beta strand

153 – 155

3

Beta strand

168 – 175

8

Beta strand

178 – 187

10

Beta strand

192 – 197

6

Beta strand

213 – 218

6

Sequences

	Sequence		Length	Mass (Da)
	Q9HJA6-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: BB0F694447629612	FASTA	220	24,997
	10 20 30 40 50 60 METDDQYYRA IKKIKEAAEA SNRAYLTSSK LADMLGISQQ SASRIIIDLE KNGYITRTVT 70 80 90 100 110 120 KRGQILNITE KGLDVLYTEF ADLSRILAIK NNVVITGTVT SGMGEGRYYV ARKQYIIQFQ 130 140 150 160 170 180 EKLGIIPYLG TLNIKVDQAS LPELRKIRGF RGIHIEGFKT EDRTFGSVKA FPAKIQNIPC 190 200 210 220 FVIMPERTVY TDVIEIISDK YLREEINLHD GDRVSVEVYT

References

[1]

"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / AMRC-C165 / DSM 1728 / IFO 15155 / JCM 9062.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AL445066 Genomic DNA. Translation: CAC12192.1.

RefSeq

NP_394524.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CTA	X-ray	2.20	A	2-220	[»]

ModBase

Search...

Genome annotation databases

GeneID

1456578.

GenomeReviews

Gene locus Ta1064 in contig AL139299_GR.

KEGG

tac:Ta1064.

NMPDR

fig|273075.1.peg.1046.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

Q9HJA6.

OMA

GAYYVRQ.

Enzyme and pathway databases

BioCyc

TACI273075:TA1064-MON.

Family and domain databases

HAMAP

MF_01285. Fused.
[Tree]

InterPro

IPR000835. HTH_MarR.
IPR002834. Riboflavin_kinase_arc.
[Graphical view]

Pfam

PF01982. CTP-dep_RFKase. 1 hit.
PF01047. MarR. 1 hit.
[Graphical view]

ProDom

PD015839. DUF120. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Entry information

Entry name

RIFK_THEAC

Accession

Primary (citable) accession number: Q9HJA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)