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Protein

Riboflavin kinase

Gene

ribK

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN).By similarity

Catalytic activityi

CTP + riboflavin = CDP + FMN.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi131 – 1311MagnesiumBy similarity
Metal bindingi133 – 1331MagnesiumBy similarity
Binding sitei188 – 1881FMNBy similarity
Binding sitei195 – 1951FMNBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi102 – 1076CDPBy similarity
Nucleotide bindingi200 – 2034CDPBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. nucleotide binding Source: UniProtKB-KW
  3. riboflavin kinase activity Source: InterPro
  4. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. FMN biosynthetic process Source: UniProtKB-UniPathway
  2. riboflavin biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

Flavoprotein, FMN, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00276; UER00929.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin kinase (EC:2.7.1.161)
Short name:
RFK
Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase
Gene namesi
Name:ribK
Ordered Locus Names:Ta1064
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
ProteomesiUP000001024: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220Riboflavin kinasePRO_0000322106Add
BLAST

Proteomic databases

PRIDEiQ9HJA6.

Interactioni

Protein-protein interaction databases

STRINGi273075.Ta1064.

Structurei

Secondary structure

1
220
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 1712Combined sources
Turni18 – 203Combined sources
Beta strandi21 – 255Combined sources
Helixi28 – 358Combined sources
Helixi39 – 5113Combined sources
Beta strandi54 – 607Combined sources
Beta strandi63 – 686Combined sources
Helixi70 – 8617Combined sources
Beta strandi93 – 997Combined sources
Helixi116 – 1227Combined sources
Beta strandi133 – 1364Combined sources
Helixi138 – 1403Combined sources
Helixi141 – 1499Combined sources
Beta strandi153 – 1553Combined sources
Beta strandi168 – 1758Combined sources
Beta strandi178 – 18710Combined sources
Beta strandi192 – 1976Combined sources
Beta strandi213 – 2186Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CTAX-ray2.20A2-220[»]
ProteinModelPortaliQ9HJA6.
SMRiQ9HJA6. Positions 5-220.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HJA6.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9292H-T-H motif-likeAdd
BLAST
Regioni93 – 220128Riboflavin kinaseAdd
BLAST

Sequence similaritiesi

Belongs to the archaeal riboflavin kinase family.Curated

Phylogenomic databases

eggNOGiCOG1339.
HOGENOMiHOG000015512.
KOiK07732.
OMAiGAYYVRQ.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.40.30.30. 1 hit.
InterProiIPR000835. HTH_MarR-typ.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023602. Riboflavin_kinase_CTP-dep.
IPR023465. Riboflavin_kinase_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01982. CTP-dep_RFKase. 1 hit.
PF01047. MarR. 1 hit.
[Graphical view]
SUPFAMiSSF82114. SSF82114. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HJA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METDDQYYRA IKKIKEAAEA SNRAYLTSSK LADMLGISQQ SASRIIIDLE
60 70 80 90 100
KNGYITRTVT KRGQILNITE KGLDVLYTEF ADLSRILAIK NNVVITGTVT
110 120 130 140 150
SGMGEGRYYV ARKQYIIQFQ EKLGIIPYLG TLNIKVDQAS LPELRKIRGF
160 170 180 190 200
RGIHIEGFKT EDRTFGSVKA FPAKIQNIPC FVIMPERTVY TDVIEIISDK
210 220
YLREEINLHD GDRVSVEVYT
Length:220
Mass (Da):24,997
Last modified:March 1, 2001 - v1
Checksum:iBB0F694447629612
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445066 Genomic DNA. Translation: CAC12192.1.
RefSeqiNP_394524.1. NC_002578.1.
WP_010901475.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC12192; CAC12192; CAC12192.
GeneIDi1456578.
KEGGitac:Ta1064.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445066 Genomic DNA. Translation: CAC12192.1.
RefSeqiNP_394524.1. NC_002578.1.
WP_010901475.1. NC_002578.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CTAX-ray2.20A2-220[»]
ProteinModelPortaliQ9HJA6.
SMRiQ9HJA6. Positions 5-220.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273075.Ta1064.

Proteomic databases

PRIDEiQ9HJA6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC12192; CAC12192; CAC12192.
GeneIDi1456578.
KEGGitac:Ta1064.

Phylogenomic databases

eggNOGiCOG1339.
HOGENOMiHOG000015512.
KOiK07732.
OMAiGAYYVRQ.

Enzyme and pathway databases

UniPathwayiUPA00276; UER00929.

Miscellaneous databases

EvolutionaryTraceiQ9HJA6.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.40.30.30. 1 hit.
InterProiIPR000835. HTH_MarR-typ.
IPR015865. Riboflavin_kinase_bac/euk.
IPR023602. Riboflavin_kinase_CTP-dep.
IPR023465. Riboflavin_kinase_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01982. CTP-dep_RFKase. 1 hit.
PF01047. MarR. 1 hit.
[Graphical view]
SUPFAMiSSF82114. SSF82114. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
    Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
    Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Entry informationi

Entry nameiRIFK_THEAC
AccessioniPrimary (citable) accession number: Q9HJA6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 1, 2001
Last modified: January 7, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.