Q9HJA4 (PURL_THEAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Ta1066

Organism

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

Taxonomic identifier

273075 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 759	759	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100527
Regions
	Nucleotide binding	120 – 131	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9HJA4 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 9A8396B684D082D3	FASTA	759	83,612
	10 20 30 40 50 60 MIFRTIRIRD ADDARLKAIS KRLGLALSLD EMKAVRSYFE RLGRDPIDAE IHAVAQSWSE 70 80 90 100 110 120 HCSYKSSKYY LKKYLGSLKT DYTILAMEDD AGVVDFDGEY AYVLKMESHN HPSAVEPYGG 130 140 150 160 170 180 AATGIGGIVR DVLCMGAQPV ALIDSLFLGD VSSDRYEGLL SPRYIFGGVV GGIRDYGNRI 190 200 210 220 230 240 GIPNVAGSLY FDKLYNSNPL VNAGCVGIVR RDRIVRSKSY KPGDVLVLMG GKTGRDGIHG 250 260 270 280 290 300 VNFASTTLGK VTKSSRLAIQ LGNPIVEQPM IKAVLEANDA GLIRAMKDLG GGGLSSAATE 310 320 330 340 350 360 MVYAGGFGAE ITLDDIKLKE SNMSGWEIWI SESQERMLME CYPEDVEKIR QIAEKWNLDF 370 380 390 400 410 420 SVIGQVTADR RIRVYYKKRK IIDMDIEFLD DSPVYQRPYR IKEVEKSVTV PQEPEDLNSF 430 440 450 460 470 480 VRDFMARLNT CARFNVVRQY DHTVRGSTIV TPFVGRPNKE THADATVIKP LENSMRGLVL 490 500 510 520 530 540 TSGSRPNMVS VDPYAGTLLT LAEAYKNILS TGGRPHSVVD ALNFGNPERE EIMGQFVESV 550 560 570 580 590 600 RAIGDFCRKM GLPVVAGNVS FYNEYRKTDI MPTPTIMMVG LIDDVRRSRT TYMKGSGNAI 610 620 630 640 650 660 YLIGEPCDNL TGSEYSRMHG YTDGFLPAPD LDELTRIRDF LSSKADMILS SHDVSSGGLF 670 680 690 700 710 720 AALSEMSFGS GIGFHVDISN VSAARPTVKL FSECGNGLVL EIAKDKEEQF VDGSNGLAIK 730 740 750 RLGETGGDRI IVDEAGLNII DVAVDDLRGA WEHGLDRYI

References

[1]

"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL445066 Genomic DNA. Translation: CAC12194.1.
RefSeq	NP_394526.1. NC_002578.1.
3D structure databases
ProteinModelPortal	Q9HJA4.
ModBase	Search...
Proteomic databases
PRIDE	Q9HJA4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1456580.
GenomeReviews	Gene locus Ta1066 in contig AL139299_GR.
KEGG	tac:Ta1066.
NMPDR	fig|273075.1.peg.1048.
Phylogenomic databases
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q9HJA4.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	TACI273075:TA1066-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_THEAC

Accession

Primary (citable) accession number: Q9HJA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program