Reviewed, UniProtKB/Swiss-Prot Q9HJ69 (G3P_THEAC)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	Ta1103

Organism

Thermoplasma acidophilum [Complete proteome] [HAMAP]

Taxonomic identifier

2303 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559		PRO_0000145737
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	138 – 140	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	192 – 193	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	139	1	Nucleophile By similarity
	Binding site	109	1	NAD; via amide nitrogen By similarity
	Binding site	167	1	NAD By similarity
	Binding site	169	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	299	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HJ69-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 8CEA4DF107909AE5	FASTA	338	37,784
	10 20 30 40 50 60 MIRVGINGYG TIGRRVARAV SLQDDMIVTG IVKTKPDYVS MVASRDFKIF VPDKSYEKAF 70 80 90 100 110 120 QDAGIKVEGT VENLIDDSEI IVDATPEGQG EKNKDLYVKR RIKAIFEGGE EPEIAQASFN 130 140 150 160 170 180 AYSNFEEARS KDYVRVVSCN TTALARTLHP LRERFGIRYV DATIVRRATD PNDSKKGPIN 190 200 210 220 230 240 AVEPSLSIPS HHAPDLRTVM SGLNVSTIAI KAPTTLMHVH AVRVELEKDA SKNDVIEAWR 250 260 270 280 290 300 SYRRIMEIGK NSGITSTGQM MDLAREFGRD RSDLYEIAVW SQSVSVQGNH VSYIQAVHQE 310 320 330 SDVVPENVDA IRSMFDIADR DRSVSMTDSK LKIEKRVF

References

[1]

"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / AMRC-C165 / DSM 1728 / IFO 15155 / JCM 9062.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL445066 Genomic DNA. Translation: CAC12230.1.
RefSeq	NP_394562.1.
3D structure databases
HSSP	HSSP built from PDB template 1CF2 based on UniProtKB P10618.
ModBase	Search...
Genome annotation databases
GeneID	1456612.
GenomeReviews	Gene locus Ta1103 in contig AL139299_GR.
KEGG	tac:Ta1103.
NMPDR	fig|273075.1.peg.1084.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9HJ69.
OMA	Q9HJ69. AIFQGGE.
Enzyme and pathway databases
BioCyc	TACI273075:TA1103-MON.
BRENDA	1.2.1.59. 435.
Family and domain databases
HAMAP	MF_00559. [Tree]
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006436. Glyceraldehyde-3-P_DH_2_arc. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
ProDom	PD007761. GAPDH_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_THEAC

Accession

Primary (citable) accession number: Q9HJ69

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)