Reviewed, UniProtKB/Swiss-Prot Q9HJ26 (DNLI_THEAC)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	Ta1148

Organism

Thermoplasma acidophilum [Complete proteome] [HAMAP]

Taxonomic identifier

2303 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	DNA ligase HAMAP MF_00407		PRO_0000059620
Sites
	Active site	250	1	N6-AMP-lysine intermediate By similarity
	Binding site	248	1	ATP By similarity
	Binding site	255	1	ATP By similarity
	Binding site	270	1	ATP By similarity
	Binding site	300	1	ATP By similarity
	Binding site	341	1	ATP By similarity
	Binding site	418	1	ATP By similarity
	Binding site	424	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HJ26-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: FFD61667871BE291	FASTA	588	66,538
	10 20 30 40 50 60 MLFSVVADAF EKMESTTKRL ELTDLLVNLL KEADDDLPLL IYLLEGKLGP DYLGIETQMS 70 80 90 100 110 120 DKLIIKALSV ASNISEDEIA KEYAKAGDIG TIAKEIAEKR SLRSLVQEEM TVKYIHDTLM 130 140 150 160 170 180 KMARTTGSGS TKARVDAYMD LFLNSTPKEI MYITRIITGK LRIGISDATI LDAIVKAFAD 190 200 210 220 230 240 EKYSEDIENA FNFHPDLGYI ASELRKGNID AILKLGPTPM IPFKVMLAER LRSVEEILEK 250 260 270 280 290 300 MGGRCAFEYK YDGMRTETHI ERGKVRLFSR GNEETTNQFP DITKAASETF KVDSAILDGE 310 320 330 340 350 360 AVPYDPDTGE LYPFQVISHR RGRKYDLDKV SSEIPITVFL FDIVYLNGRD LSKTPYTERR 370 380 390 400 410 420 KILESIFTES DSFRLAKRIE SGDPAEVHRF FNSAIEDGCE GLVAKSTSPD SFYKAGARGW 430 440 450 460 470 480 LWIKLKRDYQ AQLWDTLDLT VVGAFYGHGR RKGTYGALLL ATYNDKNDTF ETVCKLGSGF 490 500 510 520 530 540 SDDVLFSLPK KFEQYVSKEK PARVISNLEP DVWFYPAVVM EVIGAEITVS PIHTCAYGEI 550 560 570 580 EKDSGLSVRF PRFTGKWRED KKPEDSTTSR EILEMYKEQK KTITEEKS

References

[1]

"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / AMRC-C165 / DSM 1728 / IFO 15155 / JCM 9062.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL445066 Genomic DNA. Translation: CAC12273.1.
RefSeq	NP_394605.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1456651.
GenomeReviews	Gene locus Ta1148 in contig AL139299_GR.
KEGG	tac:Ta1148.
NMPDR	fig|273075.1.peg.1127.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9HJ26.
OMA	Q9HJ26. RNWLKLK.
Enzyme and pathway databases
BioCyc	TACI273075:TA1148-MON.
BRENDA	6.5.1.1. 435.
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. False negative. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_THEAC

Accession

Primary (citable) accession number: Q9HJ26

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	March 1, 2001
Last modified:	May 5, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)