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Protein

DNA ligase

Gene

lig

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei248ATPUniRule annotation1
Active sitei250N6-AMP-lysine intermediateUniRule annotation1
Binding sitei255ATPUniRule annotation1
Binding sitei270ATPUniRule annotation1
Binding sitei300ATPUniRule annotation1
Binding sitei341ATPUniRule annotation1
Binding sitei418ATPUniRule annotation1
Binding sitei424ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:Ta1148
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001024 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000596201 – 588DNA ligaseAdd BLAST588

Proteomic databases

PRIDEiQ9HJ26.

Interactioni

Protein-protein interaction databases

STRINGi273075.Ta1148.

Structurei

3D structure databases

ProteinModelPortaliQ9HJ26.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01347. Archaea.
COG1793. LUCA.
HOGENOMiHOG000036008.
KOiK10747.
OMAiPQVVIEV.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
HAMAPiMF_00407. DNA_ligase. 1 hit.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HJ26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFSVVADAF EKMESTTKRL ELTDLLVNLL KEADDDLPLL IYLLEGKLGP
60 70 80 90 100
DYLGIETQMS DKLIIKALSV ASNISEDEIA KEYAKAGDIG TIAKEIAEKR
110 120 130 140 150
SLRSLVQEEM TVKYIHDTLM KMARTTGSGS TKARVDAYMD LFLNSTPKEI
160 170 180 190 200
MYITRIITGK LRIGISDATI LDAIVKAFAD EKYSEDIENA FNFHPDLGYI
210 220 230 240 250
ASELRKGNID AILKLGPTPM IPFKVMLAER LRSVEEILEK MGGRCAFEYK
260 270 280 290 300
YDGMRTETHI ERGKVRLFSR GNEETTNQFP DITKAASETF KVDSAILDGE
310 320 330 340 350
AVPYDPDTGE LYPFQVISHR RGRKYDLDKV SSEIPITVFL FDIVYLNGRD
360 370 380 390 400
LSKTPYTERR KILESIFTES DSFRLAKRIE SGDPAEVHRF FNSAIEDGCE
410 420 430 440 450
GLVAKSTSPD SFYKAGARGW LWIKLKRDYQ AQLWDTLDLT VVGAFYGHGR
460 470 480 490 500
RKGTYGALLL ATYNDKNDTF ETVCKLGSGF SDDVLFSLPK KFEQYVSKEK
510 520 530 540 550
PARVISNLEP DVWFYPAVVM EVIGAEITVS PIHTCAYGEI EKDSGLSVRF
560 570 580
PRFTGKWRED KKPEDSTTSR EILEMYKEQK KTITEEKS
Length:588
Mass (Da):66,538
Last modified:March 1, 2001 - v1
Checksum:iFFD61667871BE291
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445066 Genomic DNA. Translation: CAC12273.1.
RefSeqiWP_010901556.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC12273; CAC12273; CAC12273.
GeneIDi1456651.
KEGGitac:Ta1148.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445066 Genomic DNA. Translation: CAC12273.1.
RefSeqiWP_010901556.1. NC_002578.1.

3D structure databases

ProteinModelPortaliQ9HJ26.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273075.Ta1148.

Proteomic databases

PRIDEiQ9HJ26.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC12273; CAC12273; CAC12273.
GeneIDi1456651.
KEGGitac:Ta1148.

Phylogenomic databases

eggNOGiarCOG01347. Archaea.
COG1793. LUCA.
HOGENOMiHOG000036008.
KOiK10747.
OMAiPQVVIEV.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
HAMAPiMF_00407. DNA_ligase. 1 hit.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNLI_THEAC
AccessioniPrimary (citable) accession number: Q9HJ26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.