Q9HIY5 (ENDA_THEAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease
EC=3.1.27.9

Alternative name(s):
tRNA-intron endonuclease

Gene names

Name:	endA
Ordered Locus Names:	Ta1191

Organism

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) [Reference proteome] [HAMAP]

Taxonomic identifier

273075 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma › Thermoplasma acidophilum

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01834
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron. HAMAP-Rule MF_01834
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA splicing, via endonucleolytic cleavage and ligation Inferred from electronic annotation. Source: HAMAP
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 289

289

tRNA-splicing endonuclease HAMAP-Rule MF_01834

PRO_0000109490

Sites

Active site

229

1

By similarity

Active site

236

1

By similarity

Active site

265

1

By similarity

Secondary structure

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289

Helix Strand Turn

Details...

Beta strand

3 – 6

4

Beta strand

9 – 14

6

Helix

19 – 26

8

Beta strand

30 – 32

3

Beta strand

35 – 37

3

Helix

40 – 48

9

Beta strand

52 – 56

5

Helix

59 – 66

8

Helix

69 – 79

11

Beta strand

84 – 88

5

Beta strand

91 – 99

9

Beta strand

102 – 106

5

Beta strand

111 – 113

3

Helix

115 – 119

5

Beta strand

124 – 129

6

Beta strand

135 – 142

8

Beta strand

159 – 161

3

Beta strand

164 – 168

5

Beta strand

175 – 179

5

Beta strand

182 – 185

4

Helix

187 – 193

7

Helix

201 – 211

11

Beta strand

215 – 218

4

Helix

220 – 222

3

Beta strand

224 – 231

8

Beta strand

238 – 243

6

Beta strand

246 – 249

4

Helix

250 – 262

13

Beta strand

266 – 273

8

Beta strand

276 – 284

9

Sequences

	Sequence		Length	Mass (Da)
	Q9HIY5 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: B0DFA251D84DBEBF	FASTA	289	33,230
	10 20 30 40 50 60 MEQGICGSHV FFIEDGKSKN YIIGKYKIGY LSGDNLILDP YECLYLYFKG RISFQNSDSF 70 80 90 100 110 120 RDLFDTVTFD RYVAYEILKN KGYRVKEDSG LIYFRKGTEK PLSLRVMREY DRIQFSDLVE 130 140 150 160 170 180 NPVDYYFTVD EEGDPTVYSS QEIFPAGRNL VSPVSAPVVR MGGRSFGAGD LEWWIGTAFH 190 200 210 220 230 240 GFRLLTENEA NYISGNHSAS QVDMVYSDLV GRGCIVKTGF KYGANFRVYL GRDSQHAEYL 250 260 270 280 VSVMPEEERW YSISRGVRVA SSVRKTMIYA SIYKNEVRYV ALKRVKDII

References

[1]	"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL445066 Genomic DNA. Translation: CAC12316.1.

RefSeq

NP_394647.1. NC_002578.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2OHC	X-ray	2.50	A/B	1-289	[»]
2OHE	X-ray	2.70	A	1-289	[»]

ProteinModelPortal

Q9HIY5.

SMR

Q9HIY5. Positions 1-289.

ModBase

Search...

Protein-protein interaction databases

STRING

273075.Ta1191.

Proteomic databases

PRIDE

Q9HIY5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAC12316; CAC12316; CAC12316.

GeneID

1456687.

KEGG

tac:Ta1191.

Phylogenomic databases

eggNOG

COG1676.

HOGENOM

HOG000112369.

KO

K01170.

OMA

PYECLYL.

ProtClustDB

PRK09300.

Family and domain databases

Gene3D

3.40.1350.10. 1 hit.

HAMAP

MF_01834. EndA_long.

InterPro

IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]

Pfam

PF01974. tRNA_int_endo. 1 hit.
[Graphical view]

SUPFAM

SSF53032. tRNA_int_endo_C. 1 hit.

TIGRFAMs

TIGR00324. endA. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q9HIY5.

Entry information

Entry name

ENDA_THEAC

Accession

Primary (citable) accession number: Q9HIY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program