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Q9HIY5 (ENDA_THEAC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease
Gene names
Name:endA
Ordered Locus Names:Ta1191
OrganismThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) [Reference proteome] [HAMAP]
Taxonomic identifier273075 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma

Protein attributes

Sequence length289 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01834

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. HAMAP-Rule MF_01834

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01834

Sequence similarities

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionLyase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA splicing, via endonucleolytic cleavage and ligation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionnucleic acid binding

Inferred from electronic annotation. Source: InterPro

tRNA-intron endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 289289tRNA-splicing endonuclease HAMAP-Rule MF_01834
PRO_0000109490

Sites

Active site2291 By similarity
Active site2361 By similarity
Active site2651 By similarity

Secondary structure

............................................................ 289
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9HIY5 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: B0DFA251D84DBEBF

FASTA28933,230
        10         20         30         40         50         60 
MEQGICGSHV FFIEDGKSKN YIIGKYKIGY LSGDNLILDP YECLYLYFKG RISFQNSDSF 

        70         80         90        100        110        120 
RDLFDTVTFD RYVAYEILKN KGYRVKEDSG LIYFRKGTEK PLSLRVMREY DRIQFSDLVE 

       130        140        150        160        170        180 
NPVDYYFTVD EEGDPTVYSS QEIFPAGRNL VSPVSAPVVR MGGRSFGAGD LEWWIGTAFH 

       190        200        210        220        230        240 
GFRLLTENEA NYISGNHSAS QVDMVYSDLV GRGCIVKTGF KYGANFRVYL GRDSQHAEYL 

       250        260        270        280 
VSVMPEEERW YSISRGVRVA SSVRKTMIYA SIYKNEVRYV ALKRVKDII 

« Hide

References

[1]"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL445066 Genomic DNA. Translation: CAC12316.1.
RefSeqNP_394647.1. NC_002578.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2OHCX-ray2.50A/B1-289[»]
2OHEX-ray2.70A1-289[»]
ProteinModelPortalQ9HIY5.
SMRQ9HIY5. Positions 1-289.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273075.Ta1191.

Proteomic databases

PRIDEQ9HIY5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC12316; CAC12316; CAC12316.
GeneID1456687.
KEGGtac:Ta1191.

Phylogenomic databases

eggNOGCOG1676.
HOGENOMHOG000112369.
KOK01170.
OMAPYECLYL.
ProtClustDBPRK09300.

Family and domain databases

Gene3D3.40.1350.10. 1 hit.
HAMAPMF_01834. EndA_long.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
SUPFAMSSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsTIGR00324. endA. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ9HIY5.

Entry information

Entry nameENDA_THEAC
AccessionPrimary (citable) accession number: Q9HIY5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references