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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei229UniRule annotation1
Active sitei236UniRule annotation1
Active sitei265UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BRENDAi4.6.1.16. 6324.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:Ta1191
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001024 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094901 – 289tRNA-splicing endonucleaseAdd BLAST289

Proteomic databases

PRIDEiQ9HIY5.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi273075.Ta1191.

Structurei

Secondary structure

1289
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Beta strandi9 – 14Combined sources6
Helixi19 – 26Combined sources8
Beta strandi30 – 32Combined sources3
Beta strandi35 – 37Combined sources3
Helixi40 – 48Combined sources9
Beta strandi52 – 56Combined sources5
Helixi59 – 66Combined sources8
Helixi69 – 79Combined sources11
Beta strandi84 – 88Combined sources5
Beta strandi91 – 99Combined sources9
Beta strandi102 – 106Combined sources5
Beta strandi111 – 113Combined sources3
Helixi115 – 119Combined sources5
Beta strandi124 – 129Combined sources6
Beta strandi135 – 142Combined sources8
Beta strandi159 – 161Combined sources3
Beta strandi164 – 168Combined sources5
Beta strandi175 – 179Combined sources5
Beta strandi182 – 185Combined sources4
Helixi187 – 193Combined sources7
Helixi201 – 211Combined sources11
Beta strandi215 – 218Combined sources4
Helixi220 – 222Combined sources3
Beta strandi224 – 231Combined sources8
Beta strandi238 – 243Combined sources6
Beta strandi246 – 249Combined sources4
Helixi250 – 262Combined sources13
Beta strandi266 – 273Combined sources8
Beta strandi276 – 284Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OHCX-ray2.50A/B1-289[»]
2OHEX-ray2.70A1-289[»]
ProteinModelPortaliQ9HIY5.
SMRiQ9HIY5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HIY5.

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01701. Archaea.
COG1676. LUCA.
HOGENOMiHOG000112369.
KOiK01170.
OMAiPYECLYL.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
HAMAPiMF_01834. EndA_long. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsiTIGR00324. endA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HIY5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQGICGSHV FFIEDGKSKN YIIGKYKIGY LSGDNLILDP YECLYLYFKG
60 70 80 90 100
RISFQNSDSF RDLFDTVTFD RYVAYEILKN KGYRVKEDSG LIYFRKGTEK
110 120 130 140 150
PLSLRVMREY DRIQFSDLVE NPVDYYFTVD EEGDPTVYSS QEIFPAGRNL
160 170 180 190 200
VSPVSAPVVR MGGRSFGAGD LEWWIGTAFH GFRLLTENEA NYISGNHSAS
210 220 230 240 250
QVDMVYSDLV GRGCIVKTGF KYGANFRVYL GRDSQHAEYL VSVMPEEERW
260 270 280
YSISRGVRVA SSVRKTMIYA SIYKNEVRYV ALKRVKDII
Length:289
Mass (Da):33,230
Last modified:March 1, 2001 - v1
Checksum:iB0DFA251D84DBEBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445066 Genomic DNA. Translation: CAC12316.1.
RefSeqiWP_010901598.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC12316; CAC12316; CAC12316.
GeneIDi1456687.
KEGGitac:Ta1191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445066 Genomic DNA. Translation: CAC12316.1.
RefSeqiWP_010901598.1. NC_002578.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OHCX-ray2.50A/B1-289[»]
2OHEX-ray2.70A1-289[»]
ProteinModelPortaliQ9HIY5.
SMRiQ9HIY5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273075.Ta1191.

Proteomic databases

PRIDEiQ9HIY5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC12316; CAC12316; CAC12316.
GeneIDi1456687.
KEGGitac:Ta1191.

Phylogenomic databases

eggNOGiarCOG01701. Archaea.
COG1676. LUCA.
HOGENOMiHOG000112369.
KOiK01170.
OMAiPYECLYL.

Enzyme and pathway databases

BRENDAi4.6.1.16. 6324.

Miscellaneous databases

EvolutionaryTraceiQ9HIY5.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
HAMAPiMF_01834. EndA_long. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsiTIGR00324. endA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENDA_THEAC
AccessioniPrimary (citable) accession number: Q9HIY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.