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Q9HIY5

- ENDA_THEAC

UniProt

Q9HIY5 - ENDA_THEAC

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Protein
tRNA-splicing endonuclease
Gene
endA, Ta1191
Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity.UniRule annotation

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei229 – 2291 By similarity
Active sitei236 – 2361 By similarity
Active sitei265 – 2651 By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease
Gene namesi
Name:endA
Ordered Locus Names:Ta1191
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
ProteomesiUP000001024: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 289289tRNA-splicing endonucleaseUniRule annotation
PRO_0000109490Add
BLAST

Proteomic databases

PRIDEiQ9HIY5.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi273075.Ta1191.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 64
Beta strandi9 – 146
Helixi19 – 268
Beta strandi30 – 323
Beta strandi35 – 373
Helixi40 – 489
Beta strandi52 – 565
Helixi59 – 668
Helixi69 – 7911
Beta strandi84 – 885
Beta strandi91 – 999
Beta strandi102 – 1065
Beta strandi111 – 1133
Helixi115 – 1195
Beta strandi124 – 1296
Beta strandi135 – 1428
Beta strandi159 – 1613
Beta strandi164 – 1685
Beta strandi175 – 1795
Beta strandi182 – 1854
Helixi187 – 1937
Helixi201 – 21111
Beta strandi215 – 2184
Helixi220 – 2223
Beta strandi224 – 2318
Beta strandi238 – 2436
Beta strandi246 – 2494
Helixi250 – 26213
Beta strandi266 – 2738
Beta strandi276 – 2849

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OHCX-ray2.50A/B1-289[»]
2OHEX-ray2.70A1-289[»]
ProteinModelPortaliQ9HIY5.
SMRiQ9HIY5. Positions 1-289.

Miscellaneous databases

EvolutionaryTraceiQ9HIY5.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000112369.
KOiK01170.
OMAiPYECLYL.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
HAMAPiMF_01834. EndA_long.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsiTIGR00324. endA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HIY5-1 [UniParc]FASTAAdd to Basket

« Hide

MEQGICGSHV FFIEDGKSKN YIIGKYKIGY LSGDNLILDP YECLYLYFKG    50
RISFQNSDSF RDLFDTVTFD RYVAYEILKN KGYRVKEDSG LIYFRKGTEK 100
PLSLRVMREY DRIQFSDLVE NPVDYYFTVD EEGDPTVYSS QEIFPAGRNL 150
VSPVSAPVVR MGGRSFGAGD LEWWIGTAFH GFRLLTENEA NYISGNHSAS 200
QVDMVYSDLV GRGCIVKTGF KYGANFRVYL GRDSQHAEYL VSVMPEEERW 250
YSISRGVRVA SSVRKTMIYA SIYKNEVRYV ALKRVKDII 289
Length:289
Mass (Da):33,230
Last modified:March 1, 2001 - v1
Checksum:iB0DFA251D84DBEBF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL445066 Genomic DNA. Translation: CAC12316.1.
RefSeqiNP_394647.1. NC_002578.1.
WP_010901598.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC12316; CAC12316; CAC12316.
GeneIDi1456687.
KEGGitac:Ta1191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL445066 Genomic DNA. Translation: CAC12316.1 .
RefSeqi NP_394647.1. NC_002578.1.
WP_010901598.1. NC_002578.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2OHC X-ray 2.50 A/B 1-289 [» ]
2OHE X-ray 2.70 A 1-289 [» ]
ProteinModelPortali Q9HIY5.
SMRi Q9HIY5. Positions 1-289.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273075.Ta1191.

Proteomic databases

PRIDEi Q9HIY5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC12316 ; CAC12316 ; CAC12316 .
GeneIDi 1456687.
KEGGi tac:Ta1191.

Phylogenomic databases

eggNOGi COG1676.
HOGENOMi HOG000112369.
KOi K01170.
OMAi PYECLYL.

Miscellaneous databases

EvolutionaryTracei Q9HIY5.

Family and domain databases

Gene3Di 3.40.1350.10. 1 hit.
HAMAPi MF_01834. EndA_long.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 1 hit.
[Graphical view ]
SUPFAMi SSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsi TIGR00324. endA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
    Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
    Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Entry informationi

Entry nameiENDA_THEAC
AccessioniPrimary (citable) accession number: Q9HIY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2001
Last modified: September 3, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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