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Q9HIY5

- ENDA_THEAC

UniProt

Q9HIY5 - ENDA_THEAC

Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity.By similarity

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei229 – 2291UniRule annotation
    Active sitei236 – 2361UniRule annotation
    Active sitei265 – 2651UniRule annotation

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
    Alternative name(s):
    tRNA-intron endonucleaseUniRule annotation
    Gene namesi
    Name:endAUniRule annotation
    Ordered Locus Names:Ta1191
    OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
    Taxonomic identifieri273075 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
    ProteomesiUP000001024: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 289289tRNA-splicing endonucleasePRO_0000109490Add
    BLAST

    Proteomic databases

    PRIDEiQ9HIY5.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi273075.Ta1191.

    Structurei

    Secondary structure

    1
    289
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 64
    Beta strandi9 – 146
    Helixi19 – 268
    Beta strandi30 – 323
    Beta strandi35 – 373
    Helixi40 – 489
    Beta strandi52 – 565
    Helixi59 – 668
    Helixi69 – 7911
    Beta strandi84 – 885
    Beta strandi91 – 999
    Beta strandi102 – 1065
    Beta strandi111 – 1133
    Helixi115 – 1195
    Beta strandi124 – 1296
    Beta strandi135 – 1428
    Beta strandi159 – 1613
    Beta strandi164 – 1685
    Beta strandi175 – 1795
    Beta strandi182 – 1854
    Helixi187 – 1937
    Helixi201 – 21111
    Beta strandi215 – 2184
    Helixi220 – 2223
    Beta strandi224 – 2318
    Beta strandi238 – 2436
    Beta strandi246 – 2494
    Helixi250 – 26213
    Beta strandi266 – 2738
    Beta strandi276 – 2849

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2OHCX-ray2.50A/B1-289[»]
    2OHEX-ray2.70A1-289[»]
    ProteinModelPortaliQ9HIY5.
    SMRiQ9HIY5. Positions 1-289.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9HIY5.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1676.
    HOGENOMiHOG000112369.
    KOiK01170.
    OMAiPYECLYL.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    HAMAPiMF_01834. EndA_long.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR023516. tRNA_splic_arch_long.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    [Graphical view]
    SUPFAMiSSF53032. SSF53032. 1 hit.
    SSF55267. SSF55267. 1 hit.
    TIGRFAMsiTIGR00324. endA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9HIY5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQGICGSHV FFIEDGKSKN YIIGKYKIGY LSGDNLILDP YECLYLYFKG    50
    RISFQNSDSF RDLFDTVTFD RYVAYEILKN KGYRVKEDSG LIYFRKGTEK 100
    PLSLRVMREY DRIQFSDLVE NPVDYYFTVD EEGDPTVYSS QEIFPAGRNL 150
    VSPVSAPVVR MGGRSFGAGD LEWWIGTAFH GFRLLTENEA NYISGNHSAS 200
    QVDMVYSDLV GRGCIVKTGF KYGANFRVYL GRDSQHAEYL VSVMPEEERW 250
    YSISRGVRVA SSVRKTMIYA SIYKNEVRYV ALKRVKDII 289
    Length:289
    Mass (Da):33,230
    Last modified:March 1, 2001 - v1
    Checksum:iB0DFA251D84DBEBF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL445066 Genomic DNA. Translation: CAC12316.1.
    RefSeqiNP_394647.1. NC_002578.1.
    WP_010901598.1. NC_002578.1.

    Genome annotation databases

    EnsemblBacteriaiCAC12316; CAC12316; CAC12316.
    GeneIDi1456687.
    KEGGitac:Ta1191.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL445066 Genomic DNA. Translation: CAC12316.1 .
    RefSeqi NP_394647.1. NC_002578.1.
    WP_010901598.1. NC_002578.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2OHC X-ray 2.50 A/B 1-289 [» ]
    2OHE X-ray 2.70 A 1-289 [» ]
    ProteinModelPortali Q9HIY5.
    SMRi Q9HIY5. Positions 1-289.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273075.Ta1191.

    Proteomic databases

    PRIDEi Q9HIY5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC12316 ; CAC12316 ; CAC12316 .
    GeneIDi 1456687.
    KEGGi tac:Ta1191.

    Phylogenomic databases

    eggNOGi COG1676.
    HOGENOMi HOG000112369.
    KOi K01170.
    OMAi PYECLYL.

    Miscellaneous databases

    EvolutionaryTracei Q9HIY5.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    HAMAPi MF_01834. EndA_long.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR023516. tRNA_splic_arch_long.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53032. SSF53032. 1 hit.
    SSF55267. SSF55267. 1 hit.
    TIGRFAMsi TIGR00324. endA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
      Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
      Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

    Entry informationi

    Entry nameiENDA_THEAC
    AccessioniPrimary (citable) accession number: Q9HIY5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3