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Q9HIY5

- ENDA_THEAC

UniProt

Q9HIY5 - ENDA_THEAC

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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei229 – 2291UniRule annotation
Active sitei236 – 2361UniRule annotation
Active sitei265 – 2651UniRule annotation

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:Ta1191
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
ProteomesiUP000001024: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 289289tRNA-splicing endonucleasePRO_0000109490Add
BLAST

Proteomic databases

PRIDEiQ9HIY5.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi273075.Ta1191.

Structurei

Secondary structure

1
289
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 64Combined sources
Beta strandi9 – 146Combined sources
Helixi19 – 268Combined sources
Beta strandi30 – 323Combined sources
Beta strandi35 – 373Combined sources
Helixi40 – 489Combined sources
Beta strandi52 – 565Combined sources
Helixi59 – 668Combined sources
Helixi69 – 7911Combined sources
Beta strandi84 – 885Combined sources
Beta strandi91 – 999Combined sources
Beta strandi102 – 1065Combined sources
Beta strandi111 – 1133Combined sources
Helixi115 – 1195Combined sources
Beta strandi124 – 1296Combined sources
Beta strandi135 – 1428Combined sources
Beta strandi159 – 1613Combined sources
Beta strandi164 – 1685Combined sources
Beta strandi175 – 1795Combined sources
Beta strandi182 – 1854Combined sources
Helixi187 – 1937Combined sources
Helixi201 – 21111Combined sources
Beta strandi215 – 2184Combined sources
Helixi220 – 2223Combined sources
Beta strandi224 – 2318Combined sources
Beta strandi238 – 2436Combined sources
Beta strandi246 – 2494Combined sources
Helixi250 – 26213Combined sources
Beta strandi266 – 2738Combined sources
Beta strandi276 – 2849Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OHCX-ray2.50A/B1-289[»]
2OHEX-ray2.70A1-289[»]
ProteinModelPortaliQ9HIY5.
SMRiQ9HIY5. Positions 1-289.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HIY5.

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000112369.
KOiK01170.
OMAiPYECLYL.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
HAMAPiMF_01834. EndA_long.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsiTIGR00324. endA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HIY5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEQGICGSHV FFIEDGKSKN YIIGKYKIGY LSGDNLILDP YECLYLYFKG
60 70 80 90 100
RISFQNSDSF RDLFDTVTFD RYVAYEILKN KGYRVKEDSG LIYFRKGTEK
110 120 130 140 150
PLSLRVMREY DRIQFSDLVE NPVDYYFTVD EEGDPTVYSS QEIFPAGRNL
160 170 180 190 200
VSPVSAPVVR MGGRSFGAGD LEWWIGTAFH GFRLLTENEA NYISGNHSAS
210 220 230 240 250
QVDMVYSDLV GRGCIVKTGF KYGANFRVYL GRDSQHAEYL VSVMPEEERW
260 270 280
YSISRGVRVA SSVRKTMIYA SIYKNEVRYV ALKRVKDII
Length:289
Mass (Da):33,230
Last modified:March 1, 2001 - v1
Checksum:iB0DFA251D84DBEBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445066 Genomic DNA. Translation: CAC12316.1.
RefSeqiNP_394647.1. NC_002578.1.
WP_010901598.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC12316; CAC12316; CAC12316.
GeneIDi1456687.
KEGGitac:Ta1191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445066 Genomic DNA. Translation: CAC12316.1 .
RefSeqi NP_394647.1. NC_002578.1.
WP_010901598.1. NC_002578.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2OHC X-ray 2.50 A/B 1-289 [» ]
2OHE X-ray 2.70 A 1-289 [» ]
ProteinModelPortali Q9HIY5.
SMRi Q9HIY5. Positions 1-289.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273075.Ta1191.

Proteomic databases

PRIDEi Q9HIY5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC12316 ; CAC12316 ; CAC12316 .
GeneIDi 1456687.
KEGGi tac:Ta1191.

Phylogenomic databases

eggNOGi COG1676.
HOGENOMi HOG000112369.
KOi K01170.
OMAi PYECLYL.

Miscellaneous databases

EvolutionaryTracei Q9HIY5.

Family and domain databases

Gene3Di 3.40.1350.10. 1 hit.
HAMAPi MF_01834. EndA_long.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 1 hit.
[Graphical view ]
SUPFAMi SSF53032. SSF53032. 1 hit.
SSF55267. SSF55267. 1 hit.
TIGRFAMsi TIGR00324. endA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
    Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
    Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Entry informationi

Entry nameiENDA_THEAC
AccessioniPrimary (citable) accession number: Q9HIY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2001
Last modified: November 26, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3