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Protein

Trehalose-6-phosphate phosphatase-related protein

Gene

Ta1209

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Removes the phosphate from trehalose 6-phosphate (Tre6P) to produce free trehalose. Also catalyzes the dephosphorylation of para-nitrophenyl phosphate (pNPP), but with lesser efficiency (in vitro).1 Publication

Catalytic activityi

Trehalose 6-phosphate + H2O = trehalose + phosphate.1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

Kcat is 10 (sec-1) and 0.8 (sec-1) for Tre6P and pNPP, respectively (at pH 8 and 50 degrees Celsius).

  1. KM=2.7 mM for Tre6P (at pH 8 and 50 degrees Celsius)1 Publication
  2. KM=18 mM for pNPP (at pH 8 and 50 degrees Celsius)1 Publication

    Pathwayi: trehalose biosynthesis

    This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei5 – 51NucleophileBy similarity
    Metal bindingi5 – 51Magnesium1 Publication
    Metal bindingi7 – 71Magnesium; via carbonyl oxygen1 Publication
    Metal bindingi177 – 1771Magnesium1 Publication

    GO - Molecular functioni

    • magnesium ion binding Source: UniProtKB
    • trehalose-phosphatase activity Source: UniProtKB

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00299.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trehalose-6-phosphate phosphatase-related protein (EC:3.1.3.12)
    Short name:
    T6PP
    Alternative name(s):
    Trehalose 6-phosphate phosphatase
    Trehalose-phosphatase
    Gene namesi
    Ordered Locus Names:Ta1209
    OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
    Taxonomic identifieri273075 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
    Proteomesi
    • UP000001024 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 229229Trehalose-6-phosphate phosphatase-related proteinPRO_0000424546Add
    BLAST

    Expressioni

    Inductioni

    By Tre6P.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi273075.Ta1209m.

    Structurei

    Secondary structure

    1
    229
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 54Combined sources
    Turni7 – 93Combined sources
    Helixi17 – 193Combined sources
    Helixi24 – 3613Combined sources
    Beta strandi37 – 426Combined sources
    Helixi47 – 537Combined sources
    Beta strandi59 – 624Combined sources
    Helixi63 – 653Combined sources
    Beta strandi67 – 704Combined sources
    Beta strandi73 – 764Combined sources
    Helixi80 – 834Combined sources
    Helixi84 – 9411Combined sources
    Helixi97 – 1004Combined sources
    Beta strandi105 – 1095Combined sources
    Beta strandi112 – 1165Combined sources
    Helixi125 – 13915Combined sources
    Beta strandi142 – 1454Combined sources
    Beta strandi147 – 1537Combined sources
    Helixi159 – 1679Combined sources
    Beta strandi172 – 1787Combined sources
    Helixi179 – 1868Combined sources
    Turni187 – 1904Combined sources
    Beta strandi191 – 1999Combined sources
    Beta strandi204 – 2096Combined sources
    Helixi210 – 22718Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1U02X-ray1.92A2-229[»]
    ProteinModelPortaliQ9HIW7.
    SMRiQ9HIW7. Positions 1-228.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9HIW7.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni5 – 73Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the trehalose phosphatase family.Curated

    Phylogenomic databases

    eggNOGiarCOG01216. Archaea.
    COG1877. LUCA.
    OMAiICYHGAC.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    InterProiIPR023214. HAD-like_dom.
    IPR006379. HAD-SF_hydro_IIB.
    IPR003337. Trehalose_PPase.
    [Graphical view]
    PfamiPF02358. Trehalose_PPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.
    TIGR00685. T6PP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9HIW7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MIFLDYDGTL VPIIMNPEES YADAGLLSLI SDLKERFDTY IVTGRSPEEI
    60 70 80 90 100
    SRFLPLDINM ICYHGACSKI NGQIVYNNGS DRFLGVFDRI YEDTRSWVSD
    110 120 130 140 150
    FPGLRIYRKN LAVLYHLGLM GADMKPKLRS RIEEIARIFG VETYYGKMII
    160 170 180 190 200
    ELRVPGVNKG SAIRSVRGER PAIIAGDDAT DEAAFEANDD ALTIKVGEGE
    210 220
    THAKFHVADY IEMRKILKFI EMLGVQKKQ
    Length:229
    Mass (Da):25,896
    Last modified:March 1, 2001 - v1
    Checksum:iDF13250C8F401B8C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL445066 Genomic DNA. Translation: CAC12334.1.

    Genome annotation databases

    EnsemblBacteriaiCAC12334; CAC12334; CAC12334.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL445066 Genomic DNA. Translation: CAC12334.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1U02X-ray1.92A2-229[»]
    ProteinModelPortaliQ9HIW7.
    SMRiQ9HIW7. Positions 1-228.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi273075.Ta1209m.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAC12334; CAC12334; CAC12334.

    Phylogenomic databases

    eggNOGiarCOG01216. Archaea.
    COG1877. LUCA.
    OMAiICYHGAC.

    Enzyme and pathway databases

    UniPathwayiUPA00299.

    Miscellaneous databases

    EvolutionaryTraceiQ9HIW7.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    InterProiIPR023214. HAD-like_dom.
    IPR006379. HAD-SF_hydro_IIB.
    IPR003337. Trehalose_PPase.
    [Graphical view]
    PfamiPF02358. Trehalose_PPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.
    TIGR00685. T6PP. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiOTSBH_THEAC
    AccessioniPrimary (citable) accession number: Q9HIW7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 11, 2013
    Last sequence update: March 1, 2001
    Last modified: September 7, 2016
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.