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Protein

Trehalose-6-phosphate phosphatase-related protein

Gene

Ta1209

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Removes the phosphate from trehalose 6-phosphate (Tre6P) to produce free trehalose. Also catalyzes the dephosphorylation of para-nitrophenyl phosphate (pNPP), but with lesser efficiency (in vitro).1 Publication

Catalytic activityi

Trehalose 6-phosphate + H2O = trehalose + phosphate.1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

Kcat is 10 (sec-1) and 0.8 (sec-1) for Tre6P and pNPP, respectively (at pH 8 and 50 degrees Celsius).

  1. KM=2.7 mM for Tre6P (at pH 8 and 50 degrees Celsius)1 Publication
  2. KM=18 mM for pNPP (at pH 8 and 50 degrees Celsius)1 Publication

    Pathwayi: trehalose biosynthesis

    This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei5NucleophileBy similarity1
    Metal bindingi5Magnesium1 Publication1
    Metal bindingi7Magnesium; via carbonyl oxygen1 Publication1
    Metal bindingi177Magnesium1 Publication1

    GO - Molecular functioni

    • magnesium ion binding Source: UniProtKB
    • trehalose-phosphatase activity Source: UniProtKB

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00299.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trehalose-6-phosphate phosphatase-related protein (EC:3.1.3.12)
    Short name:
    T6PP
    Alternative name(s):
    Trehalose 6-phosphate phosphatase
    Trehalose-phosphatase
    Gene namesi
    Ordered Locus Names:Ta1209
    OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
    Taxonomic identifieri273075 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
    Proteomesi
    • UP000001024 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004245461 – 229Trehalose-6-phosphate phosphatase-related proteinAdd BLAST229

    Expressioni

    Inductioni

    By Tre6P.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi273075.Ta1209m.

    Structurei

    Secondary structure

    1229
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 5Combined sources4
    Turni7 – 9Combined sources3
    Helixi17 – 19Combined sources3
    Helixi24 – 36Combined sources13
    Beta strandi37 – 42Combined sources6
    Helixi47 – 53Combined sources7
    Beta strandi59 – 62Combined sources4
    Helixi63 – 65Combined sources3
    Beta strandi67 – 70Combined sources4
    Beta strandi73 – 76Combined sources4
    Helixi80 – 83Combined sources4
    Helixi84 – 94Combined sources11
    Helixi97 – 100Combined sources4
    Beta strandi105 – 109Combined sources5
    Beta strandi112 – 116Combined sources5
    Helixi125 – 139Combined sources15
    Beta strandi142 – 145Combined sources4
    Beta strandi147 – 153Combined sources7
    Helixi159 – 167Combined sources9
    Beta strandi172 – 178Combined sources7
    Helixi179 – 186Combined sources8
    Turni187 – 190Combined sources4
    Beta strandi191 – 199Combined sources9
    Beta strandi204 – 209Combined sources6
    Helixi210 – 227Combined sources18

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1U02X-ray1.92A2-229[»]
    ProteinModelPortaliQ9HIW7.
    SMRiQ9HIW7.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9HIW7.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni5 – 7Substrate bindingBy similarity3

    Sequence similaritiesi

    Belongs to the trehalose phosphatase family.Curated

    Phylogenomic databases

    eggNOGiarCOG01216. Archaea.
    COG1877. LUCA.
    OMAiICYHGAC.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    InterProiIPR023214. HAD-like_dom.
    IPR006379. HAD-SF_hydro_IIB.
    IPR003337. Trehalose_PPase.
    [Graphical view]
    PfamiPF02358. Trehalose_PPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.
    TIGR00685. T6PP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9HIW7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MIFLDYDGTL VPIIMNPEES YADAGLLSLI SDLKERFDTY IVTGRSPEEI
    60 70 80 90 100
    SRFLPLDINM ICYHGACSKI NGQIVYNNGS DRFLGVFDRI YEDTRSWVSD
    110 120 130 140 150
    FPGLRIYRKN LAVLYHLGLM GADMKPKLRS RIEEIARIFG VETYYGKMII
    160 170 180 190 200
    ELRVPGVNKG SAIRSVRGER PAIIAGDDAT DEAAFEANDD ALTIKVGEGE
    210 220
    THAKFHVADY IEMRKILKFI EMLGVQKKQ
    Length:229
    Mass (Da):25,896
    Last modified:March 1, 2001 - v1
    Checksum:iDF13250C8F401B8C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL445066 Genomic DNA. Translation: CAC12334.1.

    Genome annotation databases

    EnsemblBacteriaiCAC12334; CAC12334; CAC12334.
    KEGGitac:Ta1209.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL445066 Genomic DNA. Translation: CAC12334.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1U02X-ray1.92A2-229[»]
    ProteinModelPortaliQ9HIW7.
    SMRiQ9HIW7.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi273075.Ta1209m.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAC12334; CAC12334; CAC12334.
    KEGGitac:Ta1209.

    Phylogenomic databases

    eggNOGiarCOG01216. Archaea.
    COG1877. LUCA.
    OMAiICYHGAC.

    Enzyme and pathway databases

    UniPathwayiUPA00299.

    Miscellaneous databases

    EvolutionaryTraceiQ9HIW7.

    Family and domain databases

    Gene3Di3.40.50.1000. 2 hits.
    InterProiIPR023214. HAD-like_dom.
    IPR006379. HAD-SF_hydro_IIB.
    IPR003337. Trehalose_PPase.
    [Graphical view]
    PfamiPF02358. Trehalose_PPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01484. HAD-SF-IIB. 1 hit.
    TIGR00685. T6PP. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiOTSBH_THEAC
    AccessioniPrimary (citable) accession number: Q9HIW7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 11, 2013
    Last sequence update: March 1, 2001
    Last modified: November 2, 2016
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.