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Protein

7-carboxy-7-deazaguanine synthase

Gene

queE

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.UniRule annotation

Catalytic activityi

6-carboxy-5,6,7,8-tetrahydropterin = 7-carboxy-7-carbaguanine + NH3.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • S-adenosyl-L-methionineUniRule annotationNote: Binds 1 S-adenosyl-L-methionine per subunit.UniRule annotation
  • Mg2+UniRule annotation

Pathwayi: 7-cyano-7-deazaguanine biosynthesis

This protein is involved in the pathway 7-cyano-7-deazaguanine biosynthesis, which is part of Purine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 7-cyano-7-deazaguanine biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251SubstrateUniRule annotation
Metal bindingi29 – 291Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi33 – 331Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi36 – 361Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi38 – 381MagnesiumUniRule annotation
Binding sitei68 – 681SubstrateUniRule annotation
Binding sitei70 – 701S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00391.

Names & Taxonomyi

Protein namesi
Recommended name:
7-carboxy-7-deazaguanine synthaseUniRule annotation (EC:4.3.99.3UniRule annotation)
Short name:
CDG synthaseUniRule annotation
Alternative name(s):
Archaeosine biosynthesis protein QueEUniRule annotation
Gene namesi
Name:queEUniRule annotation
Ordered Locus Names:Ta1359
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001024 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2092097-carboxy-7-deazaguanine synthasePRO_0000416223Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi273075.Ta1359.

Structurei

3D structure databases

ProteinModelPortaliQ9HII0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni10 – 123Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG02173. Archaea.
COG0602. LUCA.
HOGENOMiHOG000266145.
OMAiCDTEYAF.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00917. QueE.
InterProiIPR024924. 7-CO-7-deazaguanine_synth-like.
IPR013785. Aldolase_TIM.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF000370. QueE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HII0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLITEIFHSI QGEGPYAGLP MLFVRTNVCN IRCEWCDTKY SFYGGKEIPL
60 70 80 90 100
SELLGIVKEA KEGWVCFTGG EPLVQRDALA FVKSVVDMGK NVLIETNGTI
110 120 130 140 150
SIRNFVFSDR IFIDMDVKPP SAKVTKGFLM DNLRYLRKQD YLKIVIKDDT
160 170 180 190 200
DLDFAIDFVD RYGEGLSFVF QPAWGSDIRR IADRIVGTGY NVRVLPQIHK

IIYGDVPGV
Length:209
Mass (Da):23,642
Last modified:March 1, 2001 - v1
Checksum:iC3DDB7BAB051722E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445067 Genomic DNA. Translation: CAC12480.1.
RefSeqiWP_010901766.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC12480; CAC12480; CAC12480.
GeneIDi1456830.
KEGGitac:Ta1359.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445067 Genomic DNA. Translation: CAC12480.1.
RefSeqiWP_010901766.1. NC_002578.1.

3D structure databases

ProteinModelPortaliQ9HII0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273075.Ta1359.

Protocols and materials databases

DNASUi1456830.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC12480; CAC12480; CAC12480.
GeneIDi1456830.
KEGGitac:Ta1359.

Phylogenomic databases

eggNOGiarCOG02173. Archaea.
COG0602. LUCA.
HOGENOMiHOG000266145.
OMAiCDTEYAF.

Enzyme and pathway databases

UniPathwayiUPA00391.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00917. QueE.
InterProiIPR024924. 7-CO-7-deazaguanine_synth-like.
IPR013785. Aldolase_TIM.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF000370. QueE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
    Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
    Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.

Entry informationi

Entry nameiQUEE_THEAC
AccessioniPrimary (citable) accession number: Q9HII0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: March 1, 2001
Last modified: February 17, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.