Q9HIH8 (GUAAB_THEAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit B
EC=6.3.5.2

Alternative name(s):
GMP synthetase

Gene names

Name:	guaAB
Ordered Locus Names:	Ta1361

Organism

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

Taxonomic identifier

273075 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_0000140253
Regions
	Domain	1 – 187	187	GMPS ATP-PPase
	Nucleotide binding	27 – 33	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HIH8 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 2F066D2B307D3BFF	FASTA	310	35,054
	10 20 30 40 50 60 MSTSSYIDQI KNEISEKVKG RAIIAVSGGQ DSSLLSVLAS QVLGDRLLCV FVDTGLIRIG 70 80 90 100 110 120 EVDRVRKFFE EHAMNYRIVD ASKRFLEALK GITDPEEKRK IIGKMFIDVL NEEAENFHAE 130 140 150 160 170 180 YLLQGTIAPD WIESGGQKRD TIKSHHNVGG LPKEMKLKLV EPLRDFYKDE IRAMSRELGL 190 200 210 220 230 240 RTDLQPFPGP GLAVRIVGEI TPEKLDLLRR ATRIVEDKIE SALKPEERPW QYFAVLLPVK 250 260 270 280 290 300 TTGVHGDRRA YGYTVAVRMI DSIDAMTGTF TKPSWDLLED IANTITDEIP DINRVVYDIT 310 NKPPATIEWE

References

[1]	"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL445067 Genomic DNA. Translation: CAC12482.1.
RefSeq	NP_394817.1. NC_002578.1.
3D structure databases
ProteinModelPortal	Q9HIH8.
SMR	Q9HIH8. Positions 1-310.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1456831.
GenomeReviews	Gene locus Ta1361 in contig AL139299_GR.
KEGG	tac:Ta1361.
NMPDR	fig|273075.1.peg.1339.
Phylogenomic databases
HOGENOM	HBG391775.
OMA	QGTIAPD.
ProtClustDB	PRK00919.
Enzyme and pathway databases
BioCyc	TACI273075:TA1361-MONOMER.
Family and domain databases
HAMAP	MF_00345. GMP_synthase_B. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR014729. Rossmann-like_a/b/a_fold. IPR011063. tRNA-lysidine/thiocyt_synth. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF01171. ATP_bind_3. 1 hit. PF00958. GMP_synt_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit.
PROSITE	PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAB_THEAC

Accession

Primary (citable) accession number: Q9HIH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program