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Q9HIC2

- PGMI_THEAC

UniProt

Q9HIC2 - PGMI_THEAC

Protein

Bifunctional phosphoglucose/phosphomannose isomerase

Gene

Ta1419

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the isomerization of both glucose 6-phosphate and epimeric mannose 6-phosphate at a similar catalytic efficiency.

    Catalytic activityi

    D-glucose 6-phosphate = D-fructose 6-phosphate.
    D-mannose 6-phosphate = D-fructose 6-phosphate.

    Enzyme regulationi

    Inhibited by erythrose 4-phosphate and 6-phosphogluconate.

    Kineticsi

    1. KM=0.72 mM for glucose 6-phosphate1 Publication
    2. KM=0.2 mM for fructose 6-phosphate1 Publication
    3. KM=0.25 mM for mannose 6-phosphate1 Publication

    Vmax=83 mmol/min/mg enzyme with glucose 6-phosphate as substrate (at 50 degrees Celsius)1 Publication

    Vmax=57 mmol/min/mg enzyme with fructose 6-phosphate as substrate (at 50 degrees Celsius)1 Publication

    Vmax=75 mmol/min/mg enzyme with mannose 6-phosphate as substrate (at 50 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 7.6.1 Publication

    Temperature dependencei

    Optimum temperature is 75 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei202 – 2021Proton acceptorBy similarity
    Active sitei218 – 2181Proton donorBy similarity
    Active sitei306 – 3061Proton acceptorBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. glucose-6-phosphate isomerase activity Source: UniProtKB-EC
    3. mannose-6-phosphate isomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Isomerase

    Enzyme and pathway databases

    BRENDAi5.3.1.8. 6324.
    SABIO-RKQ9HIC2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional phosphoglucose/phosphomannose isomerase
    Alternative name(s):
    Glucose-6-phosphate isomerase (EC:5.3.1.9)
    Short name:
    GPI
    Mannose-6-phosphate isomerase (EC:5.3.1.8)
    Phosphoglucose isomerase
    Short name:
    PGI
    Phosphomannose isomerase
    Short name:
    PMI
    Gene namesi
    Ordered Locus Names:Ta1419
    OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
    Taxonomic identifieri273075 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
    ProteomesiUP000001024: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 310310Bifunctional phosphoglucose/phosphomannose isomerasePRO_0000227545Add
    BLAST

    Proteomic databases

    PRIDEiQ9HIC2.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi273075.Ta1419.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HIC2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 152131SISPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PGI/PMI family.Curated
    Contains 1 SIS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0166.
    HOGENOMiHOG000020037.
    KOiK15916.
    OMAiIAERWKG.

    Family and domain databases

    InterProiIPR011857. Glu6P/Mann6P_isomerase.
    IPR019490. Glu6P/Mann6P_isomerase_C.
    IPR001347. SIS.
    [Graphical view]
    PfamiPF10432. bact-PGI_C. 1 hit.
    PF01380. SIS. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02128. G6PI_arch. 1 hit.
    PROSITEiPS51464. SIS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HIC2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRSHCMEFS EELKTLKDQV RFDGSFRTGT FSNIVIAGMG GSGIAGRIFS    50
    EMYSAKPVFV CDDYHIPEFV DGNTEFIAVS YSGNTEETLS AAEEAIKKGA 100
    KVHAITSGGR LSEMGVDTIK IPGGLQPRSA VGYLTMPIIN TFIRPKHEDI 150
    EEAAGLLSDL DKNNTVQENI ATEIYAGRRI PVIYGSTPYR SVAYRWKTQF 200
    NENAKILAYS NYFSELNHND TMPLRDTYRK DEFYFMAFDS TDERIRKRIE 250
    VTQKITGTSF KKIEARGSSL IARIFYLIHF GDYVTYHLAR IRNVDPQDVS 300
    AIEDLKKRIS 310
    Length:310
    Mass (Da):35,045
    Last modified:March 1, 2001 - v1
    Checksum:iA88423F901339B1F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL445067 Genomic DNA. Translation: CAC12539.1.
    RefSeqiNP_394873.1. NC_002578.1.
    WP_010901822.1. NC_002578.1.

    Genome annotation databases

    EnsemblBacteriaiCAC12539; CAC12539; CAC12539.
    GeneIDi1456878.
    KEGGitac:Ta1419.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL445067 Genomic DNA. Translation: CAC12539.1 .
    RefSeqi NP_394873.1. NC_002578.1.
    WP_010901822.1. NC_002578.1.

    3D structure databases

    ProteinModelPortali Q9HIC2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273075.Ta1419.

    Proteomic databases

    PRIDEi Q9HIC2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC12539 ; CAC12539 ; CAC12539 .
    GeneIDi 1456878.
    KEGGi tac:Ta1419.

    Phylogenomic databases

    eggNOGi COG0166.
    HOGENOMi HOG000020037.
    KOi K15916.
    OMAi IAERWKG.

    Enzyme and pathway databases

    BRENDAi 5.3.1.8. 6324.
    SABIO-RK Q9HIC2.

    Family and domain databases

    InterProi IPR011857. Glu6P/Mann6P_isomerase.
    IPR019490. Glu6P/Mann6P_isomerase_C.
    IPR001347. SIS.
    [Graphical view ]
    Pfami PF10432. bact-PGI_C. 1 hit.
    PF01380. SIS. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02128. G6PI_arch. 1 hit.
    PROSITEi PS51464. SIS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
      Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
      Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
    2. "Bifunctional phosphoglucose/phosphomannose isomerases from the archaea Aeropyrum pernix and Thermoplasma acidophilum constitute a novel enzyme family within the phosphoglucose isomerase superfamily."
      Hansen T., Wendor ff D., Schoenheit P.
      J. Biol. Chem. 279:2262-2272(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiPGMI_THEAC
    AccessioniPrimary (citable) accession number: Q9HIC2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2006
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3