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Protein

Bifunctional phosphoglucose/phosphomannose isomerase

Gene

Ta1419

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of both glucose 6-phosphate and epimeric mannose 6-phosphate at a similar catalytic efficiency.

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.
D-mannose 6-phosphate = D-fructose 6-phosphate.

Enzyme regulationi

Inhibited by erythrose 4-phosphate and 6-phosphogluconate.

Kineticsi

  1. KM=0.72 mM for glucose 6-phosphate1 Publication
  2. KM=0.2 mM for fructose 6-phosphate1 Publication
  3. KM=0.25 mM for mannose 6-phosphate1 Publication
  1. Vmax=83 mmol/min/mg enzyme with glucose 6-phosphate as substrate (at 50 degrees Celsius)1 Publication
  2. Vmax=57 mmol/min/mg enzyme with fructose 6-phosphate as substrate (at 50 degrees Celsius)1 Publication
  3. Vmax=75 mmol/min/mg enzyme with mannose 6-phosphate as substrate (at 50 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 7.6.1 Publication

Temperature dependencei

Optimum temperature is 75 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei202Proton acceptorBy similarity1
Active sitei218Proton donorBy similarity1
Active sitei306Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase

Enzyme and pathway databases

BioCyciTACI273075:G13HZ-1439-MONOMER.
BRENDAi5.3.1.8. 6324.
5.3.1.9. 6324.
SABIO-RKiQ9HIC2.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional phosphoglucose/phosphomannose isomerase
Alternative name(s):
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Phosphoglucose isomerase
Short name:
PGI
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Ordered Locus Names:Ta1419
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001024 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002275451 – 310Bifunctional phosphoglucose/phosphomannose isomeraseAdd BLAST310

Proteomic databases

PRIDEiQ9HIC2.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi273075.Ta1419.

Structurei

3D structure databases

ProteinModelPortaliQ9HIC2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 152SISPROSITE-ProRule annotationAdd BLAST131

Sequence similaritiesi

Belongs to the PGI/PMI family.Curated

Phylogenomic databases

eggNOGiarCOG00052. Archaea.
COG0166. LUCA.
HOGENOMiHOG000020037.
KOiK15916.
OMAiPELNHNE.
OrthoDBiPOG093Z0B0H.

Family and domain databases

CDDicd05637. SIS_PGI_PMI_2. 1 hit.
InterProiView protein in InterPro
IPR011857. Glu6P/Mann6P_isomerase.
IPR019490. Glu6P/Mann6P_isomerase_C.
IPR001347. SIS.
PfamiView protein in Pfam
PF10432. bact-PGI_C. 1 hit.
TIGRFAMsiTIGR02128. G6PI_arch. 1 hit.
PROSITEiView protein in PROSITE
PS51464. SIS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HIC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRSHCMEFS EELKTLKDQV RFDGSFRTGT FSNIVIAGMG GSGIAGRIFS
60 70 80 90 100
EMYSAKPVFV CDDYHIPEFV DGNTEFIAVS YSGNTEETLS AAEEAIKKGA
110 120 130 140 150
KVHAITSGGR LSEMGVDTIK IPGGLQPRSA VGYLTMPIIN TFIRPKHEDI
160 170 180 190 200
EEAAGLLSDL DKNNTVQENI ATEIYAGRRI PVIYGSTPYR SVAYRWKTQF
210 220 230 240 250
NENAKILAYS NYFSELNHND TMPLRDTYRK DEFYFMAFDS TDERIRKRIE
260 270 280 290 300
VTQKITGTSF KKIEARGSSL IARIFYLIHF GDYVTYHLAR IRNVDPQDVS
310
AIEDLKKRIS
Length:310
Mass (Da):35,045
Last modified:March 1, 2001 - v1
Checksum:iA88423F901339B1F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445067 Genomic DNA. Translation: CAC12539.1.

Genome annotation databases

EnsemblBacteriaiCAC12539; CAC12539; CAC12539.
KEGGitac:Ta1419.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPGMI_THEAC
AccessioniPrimary (citable) accession number: Q9HIC2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 1, 2001
Last modified: July 5, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families