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Q9HIC2

- PGMI_THEAC

UniProt

Q9HIC2 - PGMI_THEAC

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Protein

Bifunctional phosphoglucose/phosphomannose isomerase

Gene

Ta1419

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of both glucose 6-phosphate and epimeric mannose 6-phosphate at a similar catalytic efficiency.

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.
D-mannose 6-phosphate = D-fructose 6-phosphate.

Enzyme regulationi

Inhibited by erythrose 4-phosphate and 6-phosphogluconate.

Kineticsi

  1. KM=0.72 mM for glucose 6-phosphate1 Publication
  2. KM=0.2 mM for fructose 6-phosphate1 Publication
  3. KM=0.25 mM for mannose 6-phosphate1 Publication

Vmax=83 mmol/min/mg enzyme with glucose 6-phosphate as substrate (at 50 degrees Celsius)1 Publication

Vmax=57 mmol/min/mg enzyme with fructose 6-phosphate as substrate (at 50 degrees Celsius)1 Publication

Vmax=75 mmol/min/mg enzyme with mannose 6-phosphate as substrate (at 50 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 7.6.1 Publication

Temperature dependencei

Optimum temperature is 75 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei202 – 2021Proton acceptorBy similarity
Active sitei218 – 2181Proton donorBy similarity
Active sitei306 – 3061Proton acceptorBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glucose-6-phosphate isomerase activity Source: UniProtKB-EC
  3. mannose-6-phosphate isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BRENDAi5.3.1.8. 6324.
SABIO-RKQ9HIC2.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional phosphoglucose/phosphomannose isomerase
Alternative name(s):
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Phosphoglucose isomerase
Short name:
PGI
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Ordered Locus Names:Ta1419
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
ProteomesiUP000001024: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Bifunctional phosphoglucose/phosphomannose isomerasePRO_0000227545Add
BLAST

Proteomic databases

PRIDEiQ9HIC2.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi273075.Ta1419.

Structurei

3D structure databases

ProteinModelPortaliQ9HIC2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 152131SISPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PGI/PMI family.Curated
Contains 1 SIS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0166.
HOGENOMiHOG000020037.
KOiK15916.
OMAiIAERWKG.

Family and domain databases

InterProiIPR011857. Glu6P/Mann6P_isomerase.
IPR019490. Glu6P/Mann6P_isomerase_C.
IPR001347. SIS.
[Graphical view]
PfamiPF10432. bact-PGI_C. 1 hit.
PF01380. SIS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02128. G6PI_arch. 1 hit.
PROSITEiPS51464. SIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HIC2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRSHCMEFS EELKTLKDQV RFDGSFRTGT FSNIVIAGMG GSGIAGRIFS
60 70 80 90 100
EMYSAKPVFV CDDYHIPEFV DGNTEFIAVS YSGNTEETLS AAEEAIKKGA
110 120 130 140 150
KVHAITSGGR LSEMGVDTIK IPGGLQPRSA VGYLTMPIIN TFIRPKHEDI
160 170 180 190 200
EEAAGLLSDL DKNNTVQENI ATEIYAGRRI PVIYGSTPYR SVAYRWKTQF
210 220 230 240 250
NENAKILAYS NYFSELNHND TMPLRDTYRK DEFYFMAFDS TDERIRKRIE
260 270 280 290 300
VTQKITGTSF KKIEARGSSL IARIFYLIHF GDYVTYHLAR IRNVDPQDVS
310
AIEDLKKRIS
Length:310
Mass (Da):35,045
Last modified:March 1, 2001 - v1
Checksum:iA88423F901339B1F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445067 Genomic DNA. Translation: CAC12539.1.
RefSeqiNP_394873.1. NC_002578.1.
WP_010901822.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC12539; CAC12539; CAC12539.
GeneIDi1456878.
KEGGitac:Ta1419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445067 Genomic DNA. Translation: CAC12539.1 .
RefSeqi NP_394873.1. NC_002578.1.
WP_010901822.1. NC_002578.1.

3D structure databases

ProteinModelPortali Q9HIC2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273075.Ta1419.

Proteomic databases

PRIDEi Q9HIC2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC12539 ; CAC12539 ; CAC12539 .
GeneIDi 1456878.
KEGGi tac:Ta1419.

Phylogenomic databases

eggNOGi COG0166.
HOGENOMi HOG000020037.
KOi K15916.
OMAi IAERWKG.

Enzyme and pathway databases

BRENDAi 5.3.1.8. 6324.
SABIO-RK Q9HIC2.

Family and domain databases

InterProi IPR011857. Glu6P/Mann6P_isomerase.
IPR019490. Glu6P/Mann6P_isomerase_C.
IPR001347. SIS.
[Graphical view ]
Pfami PF10432. bact-PGI_C. 1 hit.
PF01380. SIS. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02128. G6PI_arch. 1 hit.
PROSITEi PS51464. SIS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum."
    Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W.
    Nature 407:508-513(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
  2. "Bifunctional phosphoglucose/phosphomannose isomerases from the archaea Aeropyrum pernix and Thermoplasma acidophilum constitute a novel enzyme family within the phosphoglucose isomerase superfamily."
    Hansen T., Wendor ff D., Schoenheit P.
    J. Biol. Chem. 279:2262-2272(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiPGMI_THEAC
AccessioniPrimary (citable) accession number: Q9HIC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 1, 2001
Last modified: October 1, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3