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Protein

Bifunctional phosphoglucose/phosphomannose isomerase

Gene

Ta1419

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of both glucose 6-phosphate and epimeric mannose 6-phosphate at a similar catalytic efficiency.

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.
D-mannose 6-phosphate = D-fructose 6-phosphate.

Enzyme regulationi

Inhibited by erythrose 4-phosphate and 6-phosphogluconate.

Kineticsi

  1. KM=0.72 mM for glucose 6-phosphate1 Publication
  2. KM=0.2 mM for fructose 6-phosphate1 Publication
  3. KM=0.25 mM for mannose 6-phosphate1 Publication
  1. Vmax=83 mmol/min/mg enzyme with glucose 6-phosphate as substrate (at 50 degrees Celsius)1 Publication
  2. Vmax=57 mmol/min/mg enzyme with fructose 6-phosphate as substrate (at 50 degrees Celsius)1 Publication
  3. Vmax=75 mmol/min/mg enzyme with mannose 6-phosphate as substrate (at 50 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 7.6.1 Publication

Temperature dependencei

Optimum temperature is 75 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei202Proton acceptorBy similarity1
Active sitei218Proton donorBy similarity1
Active sitei306Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Multifunctional enzyme

Enzyme and pathway databases

BRENDAi5.3.1.8 6324
5.3.1.9 6324
SABIO-RKiQ9HIC2

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional phosphoglucose/phosphomannose isomerase
Alternative name(s):
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Mannose-6-phosphate isomerase (EC:5.3.1.8)
Phosphoglucose isomerase
Short name:
PGI
Phosphomannose isomerase
Short name:
PMI
Gene namesi
Ordered Locus Names:Ta1419
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001024 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002275451 – 310Bifunctional phosphoglucose/phosphomannose isomeraseAdd BLAST310

Proteomic databases

PRIDEiQ9HIC2

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi273075.Ta1419

Structurei

3D structure databases

ProteinModelPortaliQ9HIC2
SMRiQ9HIC2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 152SISPROSITE-ProRule annotationAdd BLAST131

Sequence similaritiesi

Belongs to the PGI/PMI family.Curated

Phylogenomic databases

eggNOGiarCOG00052 Archaea
COG0166 LUCA
HOGENOMiHOG000020037
KOiK15916
OMAiNSKLMAF
OrthoDBiPOG093Z0B0H

Family and domain databases

CDDicd05017 SIS_PGI_PMI_1, 1 hit
cd05637 SIS_PGI_PMI_2, 1 hit
InterProiView protein in InterPro
IPR019490 Glu6P/Mann6P_isomerase_C
IPR011857 PGI/PMI
IPR001347 SIS
IPR035484 SIS_PGI/PMI_1
PfamiView protein in Pfam
PF10432 bact-PGI_C, 1 hit
TIGRFAMsiTIGR02128 G6PI_arch, 1 hit
PROSITEiView protein in PROSITE
PS51464 SIS, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HIC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRSHCMEFS EELKTLKDQV RFDGSFRTGT FSNIVIAGMG GSGIAGRIFS
60 70 80 90 100
EMYSAKPVFV CDDYHIPEFV DGNTEFIAVS YSGNTEETLS AAEEAIKKGA
110 120 130 140 150
KVHAITSGGR LSEMGVDTIK IPGGLQPRSA VGYLTMPIIN TFIRPKHEDI
160 170 180 190 200
EEAAGLLSDL DKNNTVQENI ATEIYAGRRI PVIYGSTPYR SVAYRWKTQF
210 220 230 240 250
NENAKILAYS NYFSELNHND TMPLRDTYRK DEFYFMAFDS TDERIRKRIE
260 270 280 290 300
VTQKITGTSF KKIEARGSSL IARIFYLIHF GDYVTYHLAR IRNVDPQDVS
310
AIEDLKKRIS
Length:310
Mass (Da):35,045
Last modified:March 1, 2001 - v1
Checksum:iA88423F901339B1F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445067 Genomic DNA Translation: CAC12539.1

Genome annotation databases

EnsemblBacteriaiCAC12539; CAC12539; CAC12539
KEGGitac:Ta1419

Similar proteinsi

Entry informationi

Entry nameiPGMI_THEAC
AccessioniPrimary (citable) accession number: Q9HIC2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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