Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CCA-adding enzyme

Gene

cca

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.UniRule annotation

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501ATP or CTPUniRule annotation
Binding sitei53 – 531ATP or CTPUniRule annotation
Metal bindingi61 – 611MagnesiumUniRule annotation
Metal bindingi63 – 631MagnesiumUniRule annotation
Metal bindingi112 – 1121MagnesiumUniRule annotation
Binding sitei135 – 1351ATP or CTPUniRule annotation
Binding sitei155 – 1551ATP or CTPUniRule annotation
Binding sitei164 – 1641ATP or CTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

RNA repair, tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CCA-adding enzymeUniRule annotation (EC:2.7.7.72UniRule annotation)
Alternative name(s):
CCA tRNA nucleotidyltransferaseUniRule annotation
tRNA CCA-pyrophosphorylaseUniRule annotation
tRNA adenylyl-/cytidylyl- transferaseUniRule annotation
tRNA nucleotidyltransferaseUniRule annotation
tRNA-NTUniRule annotation
Gene namesi
Name:ccaUniRule annotation
Ordered Locus Names:Ta1446
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001024 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 431431CCA-adding enzymePRO_0000139085Add
BLAST

Proteomic databases

PRIDEiQ9HI95.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi273075.Ta1446.

Structurei

3D structure databases

ProteinModelPortaliQ9HI95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04249. Archaea.
COG1746. LUCA.
HOGENOMiHOG000226400.
KOiK07558.
OMAiDRTPFHN.

Family and domain databases

HAMAPiMF_01264. CCA_arch. 1 hit.
InterProiIPR008229. CCA-adding_arc.
IPR011068. NuclTrfase_I_C.
IPR002934. Polymerase_NTP_transf_dom.
IPR015329. tRNA_NucTransf2.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF09249. tRNA_NucTransf2. 1 hit.
[Graphical view]
PIRSFiPIRSF005335. CCA_arch. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
TIGRFAMsiTIGR03671. cca_archaeal. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HI95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDYQEVLSR YRPTQEEENK LKIISDDIIR KINSICRSRG LRAEAVIVGS
60 70 80 90 100
YAKGTNLRDG DLDIFIAFDR DYPEEIINTE GLHIGHAVIP NGREKYAEHP
110 120 130 140 150
YVSGEIGGVK IDVVPCYKMS FGDKKISAVD RTLLHTEYVN GHLDEKGRDE
160 170 180 190 200
VRLLKIFTKS IGVYGAEART FGFSGYLCEL LVIRFGSFEN VIRYFSKAKG
210 220 230 240 250
RVLIDLDERF RDPMVLIDPV DPDRNVASPV SLESLSRMKI ASKMFLSSPD
260 270 280 290 300
EGFFQIEHNG KNVQYHDRGT CIMIYSLPKP DLTDDVIYPQ VYRFRSVLQK
310 320 330 340 350
IMESHEIRVI SSEIDVSDRI YVLIETPACA EERIRVHTGP PVDTDNAVDF
360 370 380 390 400
VNSWKARDRS RGPYIVADRL YVDVFTGQRS IEDIVRQEIF NYSIGKNLDR
410 420 430
FKKSMEIMKF NVGMKSLPVL DKFFGADVFR K
Length:431
Mass (Da):49,400
Last modified:March 1, 2001 - v1
Checksum:i59DFDFA4A0665892
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445067 Genomic DNA. Translation: CAC12566.1.
PIRiA59474.
RefSeqiWP_010901849.1. NC_002578.1.

Genome annotation databases

EnsemblBacteriaiCAC12566; CAC12566; CAC12566.
GeneIDi1456902.
KEGGitac:Ta1446.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445067 Genomic DNA. Translation: CAC12566.1.
PIRiA59474.
RefSeqiWP_010901849.1. NC_002578.1.

3D structure databases

ProteinModelPortaliQ9HI95.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273075.Ta1446.

Proteomic databases

PRIDEiQ9HI95.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC12566; CAC12566; CAC12566.
GeneIDi1456902.
KEGGitac:Ta1446.

Phylogenomic databases

eggNOGiarCOG04249. Archaea.
COG1746. LUCA.
HOGENOMiHOG000226400.
KOiK07558.
OMAiDRTPFHN.

Family and domain databases

HAMAPiMF_01264. CCA_arch. 1 hit.
InterProiIPR008229. CCA-adding_arc.
IPR011068. NuclTrfase_I_C.
IPR002934. Polymerase_NTP_transf_dom.
IPR015329. tRNA_NucTransf2.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF09249. tRNA_NucTransf2. 1 hit.
[Graphical view]
PIRSFiPIRSF005335. CCA_arch. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
TIGRFAMsiTIGR03671. cca_archaeal. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCCA_THEAC
AccessioniPrimary (citable) accession number: Q9HI95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: March 1, 2001
Last modified: November 11, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.