ID   GVPO2_HALSA             Reviewed;         111 AA.
AC   Q9HHS8; P33966;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2001, sequence version 1.
DT   27-MAR-2024, entry version 94.
DE   RecName: Full=Gas vesicle protein O2 {ECO:0000303|PubMed:21158390};
DE            Short=GvpO2 {ECO:0000303|PubMed:21158390};
GN   Name=gvpO2 {ECO:0000312|EMBL:AAG20898.1};
GN   Synonyms=c-gvpO {ECO:0000303|PubMed:1404376};
GN   OrderedLocusNames=VNG_6246G {ECO:0000312|EMBL:AAG20898.1};
OS   Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS   (Halobacterium halobium).
OG   Plasmid pNRC200.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium; Halobacterium salinarum NRC-34001.
OX   NCBI_TaxID=64091;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND GAS VESICLE GENE CLUSTER.
RC   STRAIN=NRC-817;
RX   PubMed=1404376; DOI=10.1016/0022-2836(92)90914-6;
RA   Englert C., Krueger K., Offner S., Pfeifer F.;
RT   "Three different but related gene clusters encoding gas vesicles in
RT   halophilic archaea.";
RL   J. Mol. Biol. 227:586-592(1992).
RN   [2] {ECO:0000312|EMBL:CAA64342.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION BY.
RC   STRAIN=PHH1 /PHH4;
RX   PubMed=8763925; DOI=10.1128/jb.178.14.4012-4019.1996;
RA   Krueger K., Pfeifer F.;
RT   "Transcript analysis of the c-vac region and differential synthesis of the
RT   two regulatory gas vesicle proteins GvpD and GvpE in Halobacterium
RT   salinarium PHH4.";
RL   J. Bacteriol. 178:4012-4019(1996).
RN   [3] {ECO:0000312|EMBL:AAG20898.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1; PLASMID=pNRC200;
RX   PubMed=11016950; DOI=10.1073/pnas.190337797;
RA   Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA   Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA   Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA   Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA   Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA   Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J.,
RA   Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA   DasSarma S.;
RT   "Genome sequence of Halobacterium species NRC-1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
RN   [4]
RP   PROTEIN SEQUENCE OF 63-70, SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX   PubMed=21158390; DOI=10.1021/pr1009383;
RA   Chu L.J., Chen M.C., Setter J., Tsai Y.S., Yang H., Fang X., Ting Y.S.,
RA   Shaffer S.A., Taylor G.K., von Haller P.D., Goodlett D.R., Ng W.V.;
RT   "New structural proteins of Halobacterium salinarum gas vesicle revealed by
RT   comparative proteomics analysis.";
RL   J. Proteome Res. 10:1170-1178(2011).
RN   [5]
RP   FUNCTION IN BUOYANCY, AND POSSIBLE INDUCTION BY OXYGEN LIMITATION.
RC   STRAIN=PHH1;
RX   PubMed=33711860; DOI=10.1099/00221287-143-2-467;
RA   Beard S.J., Hayes P.K., Walsby A.E.;
RT   "Growth competition between Halobacterium salinarium strain PHH1 and
RT   mutants affected in gas vesicle synthesis.";
RL   Microbiology 143:467-473(1997).
RN   [6]
RP   FUNCTION.
RC   STRAIN=PHH1;
RX   PubMed=9611808; DOI=10.1099/00221287-144-5-1331;
RA   Offner S., Ziese U., Wanner G., Typke D., Pfeifer F.;
RT   "Structural characteristics of halobacterial gas vesicles.";
RL   Microbiology 144:1331-1342(1998).
CC   -!- FUNCTION: A minor component of the gas vesicle (GV), also found in
CC       soluble extracts (PubMed:21158390). May play a role in transcription
CC       and/or RNA stability and/or in GV assembly (By similarity). Gas
CC       vesicles are hollow, gas filled proteinaceous nanostructures found in
CC       several microbial planktonic microorganisms. They allow positioning of
CC       halobacteria at the optimal depth for growth in the poorly aerated,
CC       shallow brine pools of their habitat (PubMed:33711860).
CC       {ECO:0000250|UniProtKB:O51968, ECO:0000269|PubMed:21158390,
CC       ECO:0000269|PubMed:33711860}.
CC   -!- FUNCTION: Expression of 2 c-vac DNA fragments containing 2 divergently
CC       transcribed regions (gvpE-gvpF-gvpG-gvpH-gvpI-gvpJ-gvpK-gvpL-gvpM and
CC       gvpA-gvpC-gvpN-gvpO) allows H.volcanii to produce gas vesicles.
CC       {ECO:0000269|PubMed:9611808}.
CC   -!- SUBUNIT: Forms homodimers, forms a GvpN-GvpO heterodimer, interacts
CC       with GvpC, GvpF, GvpI and GvpL, might interact with GvpA.
CC       {ECO:0000250|UniProtKB:O51968}.
CC   -!- SUBCELLULAR LOCATION: Gas vesicle {ECO:0000269|PubMed:21158390}.
CC       Cytoplasm {ECO:0000305|PubMed:21158390}.
CC   -!- INDUCTION: In PHH4 (a deletion of the p-vac locus) not transcribed in
CC       exponential phase, transcribed from stationary to mid-stationary phase.
CC       Small amounts of longer transcripts that include gvpC-gvpN-gvpO and
CC       further downstream are also seen as well as short transcripts that only
CC       hybridize to this gene (PubMed:8763925). Gas vesicles appear earlier
CC       when grown in static culture, possibly due to O(2)-limitation
CC       (PubMed:33711860). {ECO:0000269|PubMed:33711860,
CC       ECO:0000269|PubMed:8763925}.
CC   -!- MISCELLANEOUS: Encoded in a 14-gene locus called c-vac which produces
CC       cylindrical gas vesicles only in the stationary growth phase.
CC       {ECO:0000269|PubMed:1404376, ECO:0000269|PubMed:8763925}.
CC   -!- SIMILARITY: Belongs to the gas vesicle GvpO family. {ECO:0000305}.
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DR   EMBL; X64730; CAA45995.1; -; Genomic_DNA.
DR   EMBL; X94688; CAA64342.1; -; Genomic_DNA.
DR   EMBL; AE004438; AAG20898.1; -; Genomic_DNA.
DR   RefSeq; WP_010904111.1; NZ_BK010831.1.
DR   AlphaFoldDB; Q9HHS8; -.
DR   GeneID; 68695202; -.
DR   KEGG; hal:VNG_6246G; -.
DR   PATRIC; fig|64091.14.peg.2246; -.
DR   HOGENOM; CLU_142302_0_0_2; -.
DR   InParanoid; Q9HHS8; -.
DR   OrthoDB; 205220at2157; -.
DR   Proteomes; UP000000554; Plasmid pNRC200.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0031411; C:gas vesicle; IEA:UniProtKB-SubCell.
DR   GO; GO:0031412; P:gas vesicle organization; IEA:InterPro.
DR   InterPro; IPR008634; Gas-vesicle_GvpO.
DR   Pfam; PF05800; GvpO; 1.
DR   PIRSF; PIRSF028743; GvpO_protein; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Gas vesicle; Plasmid;
KW   Reference proteome.
FT   CHAIN           1..111
FT                   /note="Gas vesicle protein O2"
FT                   /id="PRO_0000182703"
FT   REGION          1..31
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        29
FT                   /note="A -> R (in Ref. 1; CAA45995 and 2; CAA64342)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        100..101
FT                   /note="ER -> DG (in Ref. 1; CAA45995 and 2; CAA64342)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   111 AA;  12017 MW;  B2D20A3F1AE48075 CRC64;
     MDDPVADQPD GTDETPDGDT AGSSPIGLAD AQARAREAVA ELLEHPFDGI ITAESGADGG
     WRILAEVVER SAVPDTQDII GRYEITLDDG GRVTGYELRE RYRRGEMKEE L
//