Q9HHB6 (GAL1_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactokinase
EC=2.7.1.6

Alternative name(s):
Galactose kinase

Gene names

Name:	galK
Ordered Locus Names:	PF0445

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-galactose = ADP + alpha-D-galactose 1-phosphate. HAMAP MF_00246
Pathway	Carbohydrate metabolism; galactose metabolism. HAMAP MF_00246
Subunit structure	Monomer. Ref.3
Subcellular location	Cytoplasm Potential HAMAP MF_00246.
Sequence similarities	Belongs to the GHMP kinase family. GalK subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Galactose metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	galactose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW galactokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 352

352

Galactokinase HAMAP MF_00246

PRO_0000184641

Regions

Nucleotide binding

99 – 109

11

ATP Potential

Secondary structure

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352

Helix Strand Turn

Details...

Beta strand

6 – 15

10

Helix

20 – 22

3

Beta strand

28 – 37

10

Turn

50 – 53

4

Helix

70 – 82

13

Beta strand

94 – 98

5

Beta strand

102 – 104

3

Helix

106 – 121

16

Helix

128 – 141

14

Helix

150 – 157

8

Beta strand

162 – 167

6

Turn

168 – 171

4

Beta strand

172 – 177

6

Beta strand

182 – 192

11

Helix

196 – 214

19

Helix

219 – 221

3

Helix

224 – 228

5

Helix

232 – 256

25

Helix

260 – 276

17

Helix

283 – 294

12

Beta strand

298 – 302

5

Beta strand

307 – 316

10

Helix

317 – 319

3

Helix

320 – 334

15

Beta strand

340 – 344

5

Sequences

	Sequence		Length	Mass (Da)
	Q9HHB6 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 44413EAEDEE518B7	FASTA	352	39,375
	10 20 30 40 50 60 MSKITVKSPG RVNLIGEHTD YTYGYVMPMA IDLYTIITAE KYDKVQLYSE HFNEEKTFTL 70 80 90 100 110 120 DNLTKEGSWI DYVKGVLWVL IQEGYKIGGL KGKITGDLPL GAGLSSSASF EVGILEVLNQ 130 140 150 160 170 180 LYNLNIDPLK KALLAKKAEN EFVGVPCGIL DQFAVVFGKK DNVIFLDTQT LQYEYIPFPK 190 200 210 220 230 240 DVSVLVFYTG VKRELASSEY AERKRIAEES LRILGKESSK EVTEKDLGKL PPLHRKFFSY 250 260 270 280 290 300 IVRENARVLE VRDALKEGDI EKVGKILTTA HWDLAENYRV SCEELDFFVK KAMELGAYGA 310 320 330 340 350 RLTGAGFGGS AIALVDKDKA KTIGDAILRE YLAKFSWKAK YFVVKPSDGV GV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Verhees C.H. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed: 10430560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[3]	"Substrate specificity and mechanism from the structure of Pyrococcus furiosus galactokinase." Hartley A., Glynn S.E., Barynin V., Baker P.J., Sedelnikova S.E., Verhees C.H., de Geus D., van der Oost J., Timson D.J., Reece R.J., Rice D.W. J. Mol. Biol. 337:387-398(2004) [PubMed: 15003454] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS), SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF195244 Genomic DNA. Translation: AAG28454.1.
AE009950 Genomic DNA. Translation: AAL80569.1.

RefSeq

NP_578174.1. NC_003413.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1S4E	X-ray	2.90	A/B/C/D/E/F/G/H/I	1-352	[»]

ProteinModelPortal

Q9HHB6.

SMR

Q9HHB6. Positions 2-352.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBPYRT00000005315; EBPYRP00000005176; EBPYRG00000005314.

GeneID

1468286.

GenomeReviews

Gene locus PF0445 in contig AE009950_GR.

KEGG

pfu:PF0445.

NMPDR

fig|186497.1.peg.457.

Phylogenomic databases

GeneTree

EBGT00050000023109.

HOGENOM

HBG725121.

OMA

EYEKAQP.

PhylomeDB

Q9HHB6.

ProtClustDB

PRK03817.

Family and domain databases

HAMAP

MF_00246. Galactokinase.
[Tree]

InterPro

IPR000705. Galactokinase.
IPR022963. Galactokinase_bac.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR006204. GHMP_kinase.
IPR013750. GHMP_kinase_C.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]

Gene3D

G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.

KO

K00849.

Pfam

PF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]

PIRSF

PIRSF000530. Galactokinase. 1 hit.

PRINTS

PR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.

SUPFAM

SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.

TIGRFAMs

TIGR00131. Gal_kin. 1 hit.

PROSITE

PS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GAL1_PYRFU

Accession

Primary (citable) accession number: Q9HHB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program