Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Galactokinase

Gene

galK

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P). Is very specific for its substrate, since it is not able to use D-glucose, D-fructose, D-mannose, 2-deoxy-D-glucose, and D-glucosamine as substrates.UniRule annotation1 Publication

Catalytic activityi

ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate.UniRule annotation1 Publication

Kineticsi

  1. KM=0.21 mM for D-galactose (at 50 degrees Celsius)1 Publication
  2. KM=0.006 mM for ATP (at 50 degrees Celsius)1 Publication
  3. KM=0.27 mM for D-galactose (at 90 degrees Celsius)1 Publication
  4. KM=0.008 mM for ATP (at 90 degrees Celsius)1 Publication
  1. Vmax=3.66 µmol/min/mg enzyme (at 50 degrees Celsius)1 Publication
  2. Vmax=43.2 µmol/min/mg enzyme (at 90 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 5.0.1 Publication

Temperature dependencei

Optimum temperature is 90 degrees Celsius.1 Publication

Pathwayi: galactose metabolism

This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei11Transition state stabilizerCurated1
Binding sitei49ATP1
Metal bindingi107Magnesium1
Metal bindingi139Magnesium1
Active sitei151Proton acceptor1 Publication1
Binding sitei200SubstrateUniRule annotation1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi101 – 107ATP7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00214.

Names & Taxonomyi

Protein namesi
Recommended name:
GalactokinaseUniRule annotation (EC:2.7.1.6UniRule annotation)
Alternative name(s):
Galactose kinaseUniRule annotation
Gene namesi
Name:galKUniRule annotation
Ordered Locus Names:PF0445
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001846411 – 352GalactokinaseAdd BLAST352

Proteomic databases

PRIDEiQ9HHB6.

Interactioni

Subunit structurei

Monomer.3 Publications

Protein-protein interaction databases

STRINGi186497.PF0445.

Structurei

Secondary structure

1352
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 15Combined sources10
Helixi20 – 22Combined sources3
Beta strandi28 – 37Combined sources10
Turni50 – 53Combined sources4
Helixi70 – 82Combined sources13
Beta strandi94 – 98Combined sources5
Beta strandi102 – 104Combined sources3
Helixi106 – 121Combined sources16
Helixi128 – 141Combined sources14
Helixi150 – 157Combined sources8
Beta strandi162 – 167Combined sources6
Turni168 – 171Combined sources4
Beta strandi172 – 177Combined sources6
Beta strandi182 – 192Combined sources11
Helixi196 – 214Combined sources19
Helixi219 – 221Combined sources3
Helixi224 – 228Combined sources5
Helixi232 – 256Combined sources25
Helixi260 – 276Combined sources17
Helixi283 – 294Combined sources12
Beta strandi298 – 302Combined sources5
Beta strandi307 – 316Combined sources10
Helixi317 – 319Combined sources3
Helixi320 – 334Combined sources15
Beta strandi340 – 344Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S4EX-ray2.90A/B/C/D/E/F/G/H/I1-352[»]
ProteinModelPortaliQ9HHB6.
SMRiQ9HHB6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HHB6.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni17 – 20Substrate binding4

Sequence similaritiesi

Belongs to the GHMP kinase family. GalK subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01029. Archaea.
COG0153. LUCA.
HOGENOMiHOG000241101.
KOiK00849.
OMAiELMTQSH.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00246. Galactokinase. 1 hit.
InterProiIPR000705. Galactokinase.
IPR022963. Galactokinase_bac.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457. PTHR10457. 1 hit.
PTHR10457:SF6. PTHR10457:SF6. 1 hit.
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000530. Galactokinase. 1 hit.
PRINTSiPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00131. gal_kin. 1 hit.
PROSITEiPS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HHB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKITVKSPG RVNLIGEHTD YTYGYVMPMA IDLYTIITAE KYDKVQLYSE
60 70 80 90 100
HFNEEKTFTL DNLTKEGSWI DYVKGVLWVL IQEGYKIGGL KGKITGDLPL
110 120 130 140 150
GAGLSSSASF EVGILEVLNQ LYNLNIDPLK KALLAKKAEN EFVGVPCGIL
160 170 180 190 200
DQFAVVFGKK DNVIFLDTQT LQYEYIPFPK DVSVLVFYTG VKRELASSEY
210 220 230 240 250
AERKRIAEES LRILGKESSK EVTEKDLGKL PPLHRKFFSY IVRENARVLE
260 270 280 290 300
VRDALKEGDI EKVGKILTTA HWDLAENYRV SCEELDFFVK KAMELGAYGA
310 320 330 340 350
RLTGAGFGGS AIALVDKDKA KTIGDAILRE YLAKFSWKAK YFVVKPSDGV

GV
Length:352
Mass (Da):39,375
Last modified:March 1, 2001 - v1
Checksum:i44413EAEDEE518B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195244 Genomic DNA. Translation: AAG28454.1.
AE009950 Genomic DNA. Translation: AAL80569.1.
RefSeqiWP_011011563.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80569; AAL80569; PF0445.
GeneIDi1468286.
KEGGipfu:PF0445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195244 Genomic DNA. Translation: AAG28454.1.
AE009950 Genomic DNA. Translation: AAL80569.1.
RefSeqiWP_011011563.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S4EX-ray2.90A/B/C/D/E/F/G/H/I1-352[»]
ProteinModelPortaliQ9HHB6.
SMRiQ9HHB6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0445.

Proteomic databases

PRIDEiQ9HHB6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80569; AAL80569; PF0445.
GeneIDi1468286.
KEGGipfu:PF0445.

Phylogenomic databases

eggNOGiarCOG01029. Archaea.
COG0153. LUCA.
HOGENOMiHOG000241101.
KOiK00849.
OMAiELMTQSH.

Enzyme and pathway databases

UniPathwayiUPA00214.

Miscellaneous databases

EvolutionaryTraceiQ9HHB6.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPiMF_00246. Galactokinase. 1 hit.
InterProiIPR000705. Galactokinase.
IPR022963. Galactokinase_bac.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457. PTHR10457. 1 hit.
PTHR10457:SF6. PTHR10457:SF6. 1 hit.
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000530. Galactokinase. 1 hit.
PRINTSiPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsiTIGR00131. gal_kin. 1 hit.
PROSITEiPS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGAL1_PYRFU
AccessioniPrimary (citable) accession number: Q9HHB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.