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Protein

Glutamate 5-kinase

Gene

proB

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei17ATPUniRule annotation1
Binding sitei64SubstrateUniRule annotation1
Binding sitei151SubstrateUniRule annotation1
Binding sitei165Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi185 – 186ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMACE188937:G1FZT-4367-MONOMER
UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:MA_4101
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001097651 – 385Glutamate 5-kinaseAdd BLAST385

Proteomic databases

PRIDEiQ9HHA0

Interactioni

Protein-protein interaction databases

STRINGi188937.MA4101

Structurei

3D structure databases

ProteinModelPortaliQ9HHA0
SMRiQ9HHA0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini291 – 367PUAUniRule annotationAdd BLAST77

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00864 Archaea
COG0263 LUCA
InParanoidiQ9HHA0
KOiK00931
OMAiTHEIRFG
OrthoDBiPOG093Z03ES
PhylomeDBiQ9HHA0

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS50890 PUA, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HHA0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDREQFFRD VNKIVIKIGT SSITRKGCDH TKENCNIDPA FMESIAAQVY
60 70 80 90 100
ELRKHGKEVI LVSSGAIGVG LNELGIAPKP REIPIRQAAA AVGQSILMQD
110 120 130 140 150
WSRAFSKYGM KVAQILLTYE FYSDRVTYLN LRNSISTLLE YEVVPIINEN
160 170 180 190 200
DCTCTNEIEA IFGDNDKLSA MVASKIDADL LIILSDIDGL FDRNPKTHSD
210 220 230 240 250
AKLLTLIKKI TPEIESYGGD PTNFKGVGGM RTKIKAAKIC SMAGCYVVIA
260 270 280 290 300
NSEIEDVVTK ILSGEEIGTL FLAERHIQKN RARWIILARA SGTVRVDAGA
310 320 330 340 350
KAAVLGKNSL LPAGIVDIEG TFDRGDVVKL ECEGKVFAKG ITNYTSKELI
360 370 380
KIKGAQTNQI DNILGYNNYD NVIKKENIGI LEGIN
Length:385
Mass (Da):42,254
Last modified:March 1, 2001 - v1
Checksum:i84AA5067126653C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305580 Genomic DNA Translation: AAG22032.1
AE010299 Genomic DNA Translation: AAM07449.1
RefSeqiWP_011023993.1, NC_003552.1

Genome annotation databases

EnsemblBacteriaiAAM07449; AAM07449; MA_4101
GeneIDi1475995
KEGGimac:MA_4101

Similar proteinsi

Entry informationi

Entry nameiPROB_METAC
AccessioniPrimary (citable) accession number: Q9HHA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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