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Q9HHA0 (PROB_METAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamate 5-kinase
EC=2.7.2.11
Alternative name(s):
Gamma-glutamyl kinase
Short name=GK
Gene names
Name:proB
Ordered Locus Names:MA_4101
OrganismMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) [Reference proteome] [HAMAP]
Taxonomic identifier188937 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina ›

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the glutamate 5-kinase family.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Proline biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-proline biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: InterPro

glutamate 5-kinase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 385385Glutamate 5-kinase HAMAP-Rule MF_00456
PRO_0000109765

Regions

Domain291 – 36777PUA
Nucleotide binding185 – 1862ATP By similarity

Sites

Binding site171ATP By similarity
Binding site641Substrate By similarity
Binding site1511Substrate By similarity
Binding site1651Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9HHA0 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 84AA5067126653C6

FASTA38542,254
        10         20         30         40         50         60 
MTDREQFFRD VNKIVIKIGT SSITRKGCDH TKENCNIDPA FMESIAAQVY ELRKHGKEVI 

        70         80         90        100        110        120 
LVSSGAIGVG LNELGIAPKP REIPIRQAAA AVGQSILMQD WSRAFSKYGM KVAQILLTYE 

       130        140        150        160        170        180 
FYSDRVTYLN LRNSISTLLE YEVVPIINEN DCTCTNEIEA IFGDNDKLSA MVASKIDADL 

       190        200        210        220        230        240 
LIILSDIDGL FDRNPKTHSD AKLLTLIKKI TPEIESYGGD PTNFKGVGGM RTKIKAAKIC 

       250        260        270        280        290        300 
SMAGCYVVIA NSEIEDVVTK ILSGEEIGTL FLAERHIQKN RARWIILARA SGTVRVDAGA 

       310        320        330        340        350        360 
KAAVLGKNSL LPAGIVDIEG TFDRGDVVKL ECEGKVFAKG ITNYTSKELI KIKGAQTNQI 

       370        380 
DNILGYNNYD NVIKKENIGI LEGIN

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF305580 Genomic DNA. Translation: AAG22032.1.
AE010299 Genomic DNA. Translation: AAM07449.1.
RefSeqNP_618969.1. NC_003552.1.

3D structure databases

ProteinModelPortalQ9HHA0.
ModBaseSearch...

Protein-protein interaction databases

STRING188937.MA4101.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM07449; AAM07449; MA_4101.
GeneID1475995.
KEGGmac:MA4101.

Phylogenomic databases

eggNOGCOG0263.
KOK00931.
OMADHLQLRG.
ProtClustDBPRK05429.

Enzyme and pathway databases

BioCycMACE188937:GI2O-4166-MONOMER.
UniPathwayUPA00098; UER00359.

Family and domain databases

Gene3D3.40.1160.10. 1 hit.
HAMAPMF_00456. ProB.
InterProIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFPIRSF000729. GK. 1 hit.
PRINTSPR00474. GLU5KINASE.
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF53633. Aa_kinase. 1 hit.
SSF88697. PUA-like. 1 hit.
TIGRFAMsTIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROB_METAC
AccessionPrimary (citable) accession number: Q9HHA0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: March 1, 2001
Last modified: May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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