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Q9HHA0

- PROB_METAC

UniProt

Q9HHA0 - PROB_METAC

Protein

Glutamate 5-kinase

Gene

proB

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

    Catalytic activityi

    ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei17 – 171ATPUniRule annotation
    Binding sitei64 – 641SubstrateUniRule annotation
    Binding sitei151 – 1511SubstrateUniRule annotation
    Binding sitei165 – 1651Substrate; via amide nitrogenUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi185 – 1862ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate 5-kinase activity Source: UniProtKB-HAMAP
    3. RNA binding Source: InterPro

    GO - Biological processi

    1. L-proline biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Proline biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMACE188937:GI2O-4151-MONOMER.
    UniPathwayiUPA00098; UER00359.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
    Alternative name(s):
    Gamma-glutamyl kinaseUniRule annotation
    Short name:
    GKUniRule annotation
    Gene namesi
    Name:proBUniRule annotation
    Ordered Locus Names:MA_4101
    OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
    Taxonomic identifieri188937 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
    ProteomesiUP000002487: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 385385Glutamate 5-kinasePRO_0000109765Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi188937.MA4101.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HHA0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini291 – 36777PUAUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glutamate 5-kinase family.UniRule annotation
    Contains 1 PUA domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0263.
    KOiK00931.
    OMAiRLMRAYE.
    PhylomeDBiQ9HHA0.

    Family and domain databases

    Gene3Di2.30.130.10. 1 hit.
    3.40.1160.10. 1 hit.
    HAMAPiMF_00456. ProB.
    InterProiIPR001048. Asp/Glu/Uridylate_kinase.
    IPR001057. Glu/AcGlu_kinase.
    IPR011529. Glu_5kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    [Graphical view]
    PfamiPF00696. AA_kinase. 1 hit.
    PF01472. PUA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000729. GK. 1 hit.
    PRINTSiPR00474. GLU5KINASE.
    SMARTiSM00359. PUA. 1 hit.
    [Graphical view]
    SUPFAMiSSF53633. SSF53633. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsiTIGR01027. proB. 1 hit.
    PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS50890. PUA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HHA0-1 [UniParc]FASTAAdd to Basket

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    MTDREQFFRD VNKIVIKIGT SSITRKGCDH TKENCNIDPA FMESIAAQVY    50
    ELRKHGKEVI LVSSGAIGVG LNELGIAPKP REIPIRQAAA AVGQSILMQD 100
    WSRAFSKYGM KVAQILLTYE FYSDRVTYLN LRNSISTLLE YEVVPIINEN 150
    DCTCTNEIEA IFGDNDKLSA MVASKIDADL LIILSDIDGL FDRNPKTHSD 200
    AKLLTLIKKI TPEIESYGGD PTNFKGVGGM RTKIKAAKIC SMAGCYVVIA 250
    NSEIEDVVTK ILSGEEIGTL FLAERHIQKN RARWIILARA SGTVRVDAGA 300
    KAAVLGKNSL LPAGIVDIEG TFDRGDVVKL ECEGKVFAKG ITNYTSKELI 350
    KIKGAQTNQI DNILGYNNYD NVIKKENIGI LEGIN 385
    Length:385
    Mass (Da):42,254
    Last modified:March 1, 2001 - v1
    Checksum:i84AA5067126653C6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF305580 Genomic DNA. Translation: AAG22032.1.
    AE010299 Genomic DNA. Translation: AAM07449.1.
    RefSeqiNP_618969.1. NC_003552.1.
    WP_011023993.1. NC_003552.1.

    Genome annotation databases

    EnsemblBacteriaiAAM07449; AAM07449; MA_4101.
    GeneIDi1475995.
    KEGGimac:MA4101.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF305580 Genomic DNA. Translation: AAG22032.1 .
    AE010299 Genomic DNA. Translation: AAM07449.1 .
    RefSeqi NP_618969.1. NC_003552.1.
    WP_011023993.1. NC_003552.1.

    3D structure databases

    ProteinModelPortali Q9HHA0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 188937.MA4101.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM07449 ; AAM07449 ; MA_4101 .
    GeneIDi 1475995.
    KEGGi mac:MA4101.

    Phylogenomic databases

    eggNOGi COG0263.
    KOi K00931.
    OMAi RLMRAYE.
    PhylomeDBi Q9HHA0.

    Enzyme and pathway databases

    UniPathwayi UPA00098 ; UER00359 .
    BioCyci MACE188937:GI2O-4151-MONOMER.

    Family and domain databases

    Gene3Di 2.30.130.10. 1 hit.
    3.40.1160.10. 1 hit.
    HAMAPi MF_00456. ProB.
    InterProi IPR001048. Asp/Glu/Uridylate_kinase.
    IPR001057. Glu/AcGlu_kinase.
    IPR011529. Glu_5kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    [Graphical view ]
    Pfami PF00696. AA_kinase. 1 hit.
    PF01472. PUA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000729. GK. 1 hit.
    PRINTSi PR00474. GLU5KINASE.
    SMARTi SM00359. PUA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53633. SSF53633. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsi TIGR01027. proB. 1 hit.
    PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS50890. PUA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Directed mutagenesis and plasmid-based complementation in the methanogenic archaeon Methanosarcina acetivorans C2A demonstrated by genetic analysis of proline biosynthesis."
      Zhang J.K., White A.K., Kuettner H.C., Boccazzi P., Metcalf W.W.
      J. Bacteriol. 184:1449-1454(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.
    2. "The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity."
      Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.
      , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B.
      Genome Res. 12:532-542(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

    Entry informationi

    Entry nameiPROB_METAC
    AccessioniPrimary (citable) accession number: Q9HHA0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3