Reviewed, UniProtKB/Swiss-Prot Q9HH99 (P5CR_METAC)

Names and origin

Protein names

Recommended name:
    Pyrroline-5-carboxylate reductase
      Short name=P5C reductase
      Short name=P5CR
    EC=1.5.1.2

Gene names

Name:	proC
Ordered Locus Names:	MA_4102

Organism

Methanosarcina acetivorans [Complete proteome] [HAMAP]

Taxonomic identifier

2214 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the pyrroline-5-carboxylate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro pyrroline-5-carboxylate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Pyrroline-5-carboxylate reductase		PRO_0000187312

Sequences

	Sequence		Length	Mass (Da)
	Q9HH99-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 50EC656AFC10B1CF	FASTA	270	27,943
	10 20 30 40 50 60 MENQKIGFIG AGKMGSALMQ GTIKAGIVTP ENIGASDVYE PFLKDLQAKL GIRVSTDNAV 70 80 90 100 110 120 IVRESDILIL AVKPQTLSSV LSNLKNEITS EKLVISIAAG VPLSTYEDAL LEGTRVVRVM 130 140 150 160 170 180 PNIAATVSEA ASGIAPGKNA TPEDLKAALE IFSAVGTAVQ VPESLMDAVT GLSGSGPAFI 190 200 210 220 230 240 FPVIEAMADG AVLEGMDRKS ALTLAAQTVL GAAKMALETG MHPGELKDMV TSPAGTTIQG 250 260 270 IHSLEEAGIR AAFMNAVIRA SERSKELGKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Directed mutagenesis and plasmid-based complementation in the methanogenic archaeon Methanosarcina acetivorans C2A demonstrated by genetic analysis of proline biosynthesis." Zhang J.K., White A.K., Kuettner H.C., Boccazzi P., Metcalf W.W. J. Bacteriol. 184:1449-1454(2002) [PubMed: 11844777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.
[2]	"The genome of Methanosarcina acetivorans reveals extensive metabolic and physiological diversity." Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W., Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J., Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P. , McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D., Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R., Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J., Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A., White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H., Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V., Zinder S.H., Lander E., Metcalf W.W., Birren B. Genome Res. 12:532-542(2002) [PubMed: 11932238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35395 / DSM 2834 / JCM 12185 / C2A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF305580 Genomic DNA. Translation: AAG22033.1. AE010299 Genomic DNA. Translation: AAM07450.1.
RefSeq	NP_618970.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1475996.
GenomeReviews	Gene locus MA_4102 in contig AE010299_GR.
KEGG	mac:MA4102.
NMPDR	fig|188937.1.peg.3996.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9HH99.
OMA	Q9HH99. APSGHSK.
Enzyme and pathway databases
BioCyc	MACE188937:MA4102-MON.
BRENDA	1.5.1.2. 275708.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. IPR004455. NADP_OxRdtase_F420. IPR000304. P5CR. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11645. P5CR. 1 hit.
Pfam	PF03807. F420_oxidored. 1 hit. [Graphical view]
PIRSF	PIRSF000193. Pyrrol-5-carb_rd. 1 hit.
TIGRFAMs	TIGR00112. proC. 1 hit.
PROSITE	PS00521. P5CR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P5CR_METAC

Accession

Primary (citable) accession number: Q9HH99

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)