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Q9HH84

- DPOL_THEG8

UniProt

Q9HH84 - DPOL_THEG8

Protein

DNA polymerase

Gene

pol

Organism
Thermococcus sp. (strain GE8)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.By similarity
    PI-TspGE8I and PI-TspGE8II are endonucleases.Curated

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
    3. endonuclease activity Source: UniProtKB-KW
    4. exonuclease activity Source: UniProtKB-KW
    5. nucleotide binding Source: InterPro

    GO - Biological processi

    1. intein-mediated protein splicing Source: InterPro
    2. intron homing Source: UniProtKB-KW

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Endonuclease, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication, Intron homing

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase (EC:2.7.7.7)
    Cleaved into the following 2 chains:
    Alternative name(s):
    Tsp-GE8 pol-1 intein
    Alternative name(s):
    Tsp-GE8 pol-2 intein
    Gene namesi
    Name:pol
    Synonyms:pol-1
    OrganismiThermococcus sp. (strain GE8)
    Taxonomic identifieri105583 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 491491DNA polymerase, 1st partPRO_0000007343Add
    BLAST
    Chaini492 – 1026535Endonuclease PI-TspGE8IPRO_0000007344Add
    BLAST
    Chaini1027 – 107549DNA polymerase, 2nd partPRO_0000007345Add
    BLAST
    Chaini1076 – 1464389Endonuclease PI-TspGE8IIPRO_0000007346Add
    BLAST
    Chaini1465 – 1699235DNA polymerase, 3rd partPRO_0000007347Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HH84.
    SMRiQ9HH84. Positions 1-1076, 1456-1696.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini770 – 903134DOD-type homing endonuclease 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1222 – 1361140DOD-type homing endonuclease 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B family.Curated
    Contains 2 DOD-type homing endonuclease domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di2.170.16.10. 4 hits.
    3.10.28.10. 3 hits.
    3.30.420.10. 1 hit.
    InterProiIPR006172. DNA-dir_DNA_pol_B.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PfamiPF00136. DNA_pol_B. 3 hits.
    PF03104. DNA_pol_B_exo1. 2 hits.
    PF14528. LAGLIDADG_3. 2 hits.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00305. HintC. 2 hits.
    SM00306. HintN. 2 hits.
    SM00486. POLBc. 1 hit.
    [Graphical view]
    SUPFAMiSSF51294. SSF51294. 4 hits.
    SSF53098. SSF53098. 1 hit.
    SSF55608. SSF55608. 2 hits.
    TIGRFAMsiTIGR01443. intein_Cterm. 2 hits.
    TIGR01445. intein_Nterm. 2 hits.
    PROSITEiPS50818. INTEIN_C_TER. 2 hits.
    PS50819. INTEIN_ENDONUCLEASE. 2 hits.
    PS50817. INTEIN_N_TER. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9HH84-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILDTDYITE DGKPVIRVFK KENGEFKIEY DRNFEPYFYA LLKDDSAIEE     50
    VKKITAKRHG TVVKVKRAEK VKKKFLGRPI EVWKLYFTHP QDVPAIRDKI 100
    REHPAVIDIY EYDIPFAKRY LIDKGLIPME GDEKLKMLAF DIETLYHEGE 150
    EFAEGPILMI SYADEEGARV ITWKKVDLPY VDVVSTEKEM IKRFLRVVKE 200
    KDPDVLITYN GDNFDFAYLK RRSEKLGVKF ILGRDGSEPK IQRMGDRFAV 250
    EVKGRIHFDL YPVIRRTINL PTYTLEAVYE AIFGKPKEKV YAEEIATAWE 300
    TGEGLERVAR YSMEDAKVTF ELGKEFFPME AQLSRLIGQS LWDVSRSSTG 350
    NLVEWFLLRK AYERNELAPN KPDERELARR RQSYAGGYVK EPERGLWNNI 400
    VYLDFRSLYP SIIITHNVSP DTLNREGCKE YDVAPQVGHK FCKDFPGFIP 450
    SLLGDLLEER QKIKRKMRAT IDPVEKKLLD YRQRAIKILA NSILPDEWLP 500
    LLVNGRLKLV RIGDFVDNTM KKGQPLENDG TEVLEVSGIE AISFNRKTKI 550
    AEIKPVKALI RHRYRGKVYD IKLSSGRNIK VTEGHSLFAF RDGELVEVTG 600
    GEIKPGDFIA VPRRVNLPER HERINLIEIL LGLPPEETSD IVLTIPVKGR 650
    KNFFKGMLRT LRWIFEEEQR PRTARRYLEH LQKLGYVKLM KRAYEIVNKE 700
    ALRNYRKLYE VLAERVKYNG NKREYLVHFN DLRNEIKFMP DEELEEWKVG 750
    TLNGFRMEPF IEVGEDFAKL LGYYVSEGYA RKQRNQKNGW SYSVKIYNND 800
    QRVLDDMEKL ASKFFGRVRR GKNYVEISRK MAYVLFESLC GTLAENKRVP 850
    EVIFTSPESV RWAFFEGYFI GDGDLHPSKR VRLSTKSEEL VNGLVVLLNS 900
    LGISAIKIRF DSGVYRVLVN EELPFLGNRK RKNAYYSHVI PKEILEETFG 950
    KQFQKNMSPA KLNEKVEKGE LDAGKARRIA WLLEGDIVLD RVEKVTVEDY 1000
    EGYVYDLSVE ENENFLAGFG MLYAHNSYYG YYGYAKARWY CRECAESVTA 1050
    WGRSYIETTI REIEEKFGFK VLYADSVAGN TEVIIRRNGK VEFVPIEKLF 1100
    QRVDYRIGEK EYCALEGVEA LTLDNRGRLV WRKVPYIMRH KTNKKIYRVW 1150
    FTNSWYLDVT EDHSLIGYLN TSKVKSEKPL KERLVEVKPR ELGEKVKSLI 1200
    TLNRAIARSI KANPIAVRLW ELIGLLVGDG NWGGHSKWAK YYVGLSCGLD 1250
    KAEIEEKVLR PLKEAGIISN YYGKSKKGDV SILSKWLAGF MVKYFKDENG 1300
    NKRIPSFMFN LPREYIEAFL RGLFSADGTV SLRRGIPEIR LTSVNRELSN 1350
    EVRKLLWLVG VSNSMFTETT PNKYLGNESG TRSIHVRIKN KHRFAKRIGF 1400
    LLDRKATKLS DNLREHTNKK MAYRYDFDLV YPKKIEEINY DRYVYDIEVE 1450
    GTHRFFANGI LVHNTDGFFA TIPGADAETV KKKAMEFLKY INAKLPGLLE 1500
    LEYEGFYVRG FFVTKKKYAV IDEEGKITTR GLEIVRRDWS EIAKETQARV 1550
    LEAILKHGDV EEAVRIVKEV TEKLSKYEVP PEKLVIHEQI TRDLKDYKAT 1600
    GPHVAVAKRL AARGIKIRPG TVISYIVLKG SGRIGDRAIP FDEFDPAKHK 1650
    YDAEYYIENQ VLPAVERILR AFGYRKEDLR YQKTKQVGLG AWLKVKGKK 1699
    Length:1,699
    Mass (Da):197,325
    Last modified:March 1, 2001 - v1
    Checksum:iF389B4351F0B12D3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ250333 Genomic DNA. Translation: CAC12850.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ250333 Genomic DNA. Translation: CAC12850.1 .

    3D structure databases

    ProteinModelPortali Q9HH84.
    SMRi Q9HH84. Positions 1-1076, 1456-1696.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.170.16.10. 4 hits.
    3.10.28.10. 3 hits.
    3.30.420.10. 1 hit.
    InterProi IPR006172. DNA-dir_DNA_pol_B.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    Pfami PF00136. DNA_pol_B. 3 hits.
    PF03104. DNA_pol_B_exo1. 2 hits.
    PF14528. LAGLIDADG_3. 2 hits.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00305. HintC. 2 hits.
    SM00306. HintN. 2 hits.
    SM00486. POLBc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51294. SSF51294. 4 hits.
    SSF53098. SSF53098. 1 hit.
    SSF55608. SSF55608. 2 hits.
    TIGRFAMsi TIGR01443. intein_Cterm. 2 hits.
    TIGR01445. intein_Nterm. 2 hits.
    PROSITEi PS50818. INTEIN_C_TER. 2 hits.
    PS50819. INTEIN_ENDONUCLEASE. 2 hits.
    PS50817. INTEIN_N_TER. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Thermococcales taxonomy and phylogeny based on the comparative use of 16S rDNA, 16S-23S rDNA intergenic spacer and family B DNA polymerase genes."
      Querellou J.J.E., Cambon M.A., Lesongeur F., Barbier G.
      Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiDPOL_THEG8
    AccessioniPrimary (citable) accession number: Q9HH84
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3