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Q9HH84

- DPOL_THEG8

UniProt

Q9HH84 - DPOL_THEG8

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Protein

DNA polymerase

Gene

pol

Organism
Thermococcus sp. (strain GE8)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.By similarity
PI-TspGE8I and PI-TspGE8II are endonucleases.Curated

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. endonuclease activity Source: UniProtKB-KW
  4. exonuclease activity Source: UniProtKB-KW
  5. nucleotide binding Source: InterPro

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
  2. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Endonuclease, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication, Intron homing

Keywords - Ligandi

DNA-binding

Protein family/group databases

MEROPSiN10.007.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase (EC:2.7.7.7)
Cleaved into the following 2 chains:
Alternative name(s):
Tsp-GE8 pol-1 intein
Alternative name(s):
Tsp-GE8 pol-2 intein
Gene namesi
Name:pol
Synonyms:pol-1
OrganismiThermococcus sp. (strain GE8)
Taxonomic identifieri105583 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491DNA polymerase, 1st partPRO_0000007343Add
BLAST
Chaini492 – 1026535Endonuclease PI-TspGE8IPRO_0000007344Add
BLAST
Chaini1027 – 107549DNA polymerase, 2nd partPRO_0000007345Add
BLAST
Chaini1076 – 1464389Endonuclease PI-TspGE8IIPRO_0000007346Add
BLAST
Chaini1465 – 1699235DNA polymerase, 3rd partPRO_0000007347Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Structurei

3D structure databases

ProteinModelPortaliQ9HH84.
SMRiQ9HH84. Positions 1-1076, 1456-1696.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini770 – 903134DOD-type homing endonuclease 1PROSITE-ProRule annotationAdd
BLAST
Domaini1222 – 1361140DOD-type homing endonuclease 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated
Contains 2 DOD-type homing endonuclease domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di2.170.16.10. 4 hits.
3.10.28.10. 3 hits.
3.30.420.10. 1 hit.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 3 hits.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14528. LAGLIDADG_3. 2 hits.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
SM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 4 hits.
SSF53098. SSF53098. 1 hit.
SSF55608. SSF55608. 2 hits.
TIGRFAMsiTIGR01443. intein_Cterm. 2 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEiPS50818. INTEIN_C_TER. 2 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9HH84-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MILDTDYITE DGKPVIRVFK KENGEFKIEY DRNFEPYFYA LLKDDSAIEE
60 70 80 90 100
VKKITAKRHG TVVKVKRAEK VKKKFLGRPI EVWKLYFTHP QDVPAIRDKI
110 120 130 140 150
REHPAVIDIY EYDIPFAKRY LIDKGLIPME GDEKLKMLAF DIETLYHEGE
160 170 180 190 200
EFAEGPILMI SYADEEGARV ITWKKVDLPY VDVVSTEKEM IKRFLRVVKE
210 220 230 240 250
KDPDVLITYN GDNFDFAYLK RRSEKLGVKF ILGRDGSEPK IQRMGDRFAV
260 270 280 290 300
EVKGRIHFDL YPVIRRTINL PTYTLEAVYE AIFGKPKEKV YAEEIATAWE
310 320 330 340 350
TGEGLERVAR YSMEDAKVTF ELGKEFFPME AQLSRLIGQS LWDVSRSSTG
360 370 380 390 400
NLVEWFLLRK AYERNELAPN KPDERELARR RQSYAGGYVK EPERGLWNNI
410 420 430 440 450
VYLDFRSLYP SIIITHNVSP DTLNREGCKE YDVAPQVGHK FCKDFPGFIP
460 470 480 490 500
SLLGDLLEER QKIKRKMRAT IDPVEKKLLD YRQRAIKILA NSILPDEWLP
510 520 530 540 550
LLVNGRLKLV RIGDFVDNTM KKGQPLENDG TEVLEVSGIE AISFNRKTKI
560 570 580 590 600
AEIKPVKALI RHRYRGKVYD IKLSSGRNIK VTEGHSLFAF RDGELVEVTG
610 620 630 640 650
GEIKPGDFIA VPRRVNLPER HERINLIEIL LGLPPEETSD IVLTIPVKGR
660 670 680 690 700
KNFFKGMLRT LRWIFEEEQR PRTARRYLEH LQKLGYVKLM KRAYEIVNKE
710 720 730 740 750
ALRNYRKLYE VLAERVKYNG NKREYLVHFN DLRNEIKFMP DEELEEWKVG
760 770 780 790 800
TLNGFRMEPF IEVGEDFAKL LGYYVSEGYA RKQRNQKNGW SYSVKIYNND
810 820 830 840 850
QRVLDDMEKL ASKFFGRVRR GKNYVEISRK MAYVLFESLC GTLAENKRVP
860 870 880 890 900
EVIFTSPESV RWAFFEGYFI GDGDLHPSKR VRLSTKSEEL VNGLVVLLNS
910 920 930 940 950
LGISAIKIRF DSGVYRVLVN EELPFLGNRK RKNAYYSHVI PKEILEETFG
960 970 980 990 1000
KQFQKNMSPA KLNEKVEKGE LDAGKARRIA WLLEGDIVLD RVEKVTVEDY
1010 1020 1030 1040 1050
EGYVYDLSVE ENENFLAGFG MLYAHNSYYG YYGYAKARWY CRECAESVTA
1060 1070 1080 1090 1100
WGRSYIETTI REIEEKFGFK VLYADSVAGN TEVIIRRNGK VEFVPIEKLF
1110 1120 1130 1140 1150
QRVDYRIGEK EYCALEGVEA LTLDNRGRLV WRKVPYIMRH KTNKKIYRVW
1160 1170 1180 1190 1200
FTNSWYLDVT EDHSLIGYLN TSKVKSEKPL KERLVEVKPR ELGEKVKSLI
1210 1220 1230 1240 1250
TLNRAIARSI KANPIAVRLW ELIGLLVGDG NWGGHSKWAK YYVGLSCGLD
1260 1270 1280 1290 1300
KAEIEEKVLR PLKEAGIISN YYGKSKKGDV SILSKWLAGF MVKYFKDENG
1310 1320 1330 1340 1350
NKRIPSFMFN LPREYIEAFL RGLFSADGTV SLRRGIPEIR LTSVNRELSN
1360 1370 1380 1390 1400
EVRKLLWLVG VSNSMFTETT PNKYLGNESG TRSIHVRIKN KHRFAKRIGF
1410 1420 1430 1440 1450
LLDRKATKLS DNLREHTNKK MAYRYDFDLV YPKKIEEINY DRYVYDIEVE
1460 1470 1480 1490 1500
GTHRFFANGI LVHNTDGFFA TIPGADAETV KKKAMEFLKY INAKLPGLLE
1510 1520 1530 1540 1550
LEYEGFYVRG FFVTKKKYAV IDEEGKITTR GLEIVRRDWS EIAKETQARV
1560 1570 1580 1590 1600
LEAILKHGDV EEAVRIVKEV TEKLSKYEVP PEKLVIHEQI TRDLKDYKAT
1610 1620 1630 1640 1650
GPHVAVAKRL AARGIKIRPG TVISYIVLKG SGRIGDRAIP FDEFDPAKHK
1660 1670 1680 1690
YDAEYYIENQ VLPAVERILR AFGYRKEDLR YQKTKQVGLG AWLKVKGKK
Length:1,699
Mass (Da):197,325
Last modified:March 1, 2001 - v1
Checksum:iF389B4351F0B12D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ250333 Genomic DNA. Translation: CAC12850.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ250333 Genomic DNA. Translation: CAC12850.1 .

3D structure databases

ProteinModelPortali Q9HH84.
SMRi Q9HH84. Positions 1-1076, 1456-1696.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi N10.007.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.170.16.10. 4 hits.
3.10.28.10. 3 hits.
3.30.420.10. 1 hit.
InterProi IPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view ]
Pfami PF00136. DNA_pol_B. 3 hits.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14528. LAGLIDADG_3. 2 hits.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
SM00486. POLBc. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 4 hits.
SSF53098. SSF53098. 1 hit.
SSF55608. SSF55608. 2 hits.
TIGRFAMsi TIGR01443. intein_Cterm. 2 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEi PS50818. INTEIN_C_TER. 2 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Thermococcales taxonomy and phylogeny based on the comparative use of 16S rDNA, 16S-23S rDNA intergenic spacer and family B DNA polymerase genes."
    Querellou J.J.E., Cambon M.A., Lesongeur F., Barbier G.
    Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiDPOL_THEG8
AccessioniPrimary (citable) accession number: Q9HH84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3