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Q9HH84

- DPOL_THEG8

UniProt

Q9HH84 - DPOL_THEG8

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Protein

DNA polymerase

Gene
pol, pol-1
Organism
Thermococcus sp. (strain GE8)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity By similarity.
PI-TspGE8I and PI-TspGE8II are endonucleases Reviewed prediction.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. endonuclease activity Source: UniProtKB-KW
  4. exonuclease activity Source: UniProtKB-KW
  5. nucleotide binding Source: InterPro

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
  2. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Endonuclease, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication, Intron homing

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase (EC:2.7.7.7)
Cleaved into the following 2 chains:
Alternative name(s):
Tsp-GE8 pol-1 intein
Alternative name(s):
Tsp-GE8 pol-2 intein
Gene namesi
Name:pol
Synonyms:pol-1
OrganismiThermococcus sp. (strain GE8)
Taxonomic identifieri105583 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491DNA polymerase, 1st partPRO_0000007343Add
BLAST
Chaini492 – 1026535Endonuclease PI-TspGE8IPRO_0000007344Add
BLAST
Chaini1027 – 107549DNA polymerase, 2nd partPRO_0000007345Add
BLAST
Chaini1076 – 1464389Endonuclease PI-TspGE8IIPRO_0000007346Add
BLAST
Chaini1465 – 1699235DNA polymerase, 3rd partPRO_0000007347Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Structurei

3D structure databases

ProteinModelPortaliQ9HH84.
SMRiQ9HH84. Positions 1-1076, 1456-1696.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini770 – 903134DOD-type homing endonuclease 1Add
BLAST
Domaini1222 – 1361140DOD-type homing endonuclease 2Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di2.170.16.10. 4 hits.
3.10.28.10. 3 hits.
3.30.420.10. 1 hit.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 3 hits.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14528. LAGLIDADG_3. 2 hits.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
SM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 4 hits.
SSF53098. SSF53098. 1 hit.
SSF55608. SSF55608. 2 hits.
TIGRFAMsiTIGR01443. intein_Cterm. 2 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEiPS50818. INTEIN_C_TER. 2 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9HH84-1 [UniParc]FASTAAdd to Basket

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MILDTDYITE DGKPVIRVFK KENGEFKIEY DRNFEPYFYA LLKDDSAIEE     50
VKKITAKRHG TVVKVKRAEK VKKKFLGRPI EVWKLYFTHP QDVPAIRDKI 100
REHPAVIDIY EYDIPFAKRY LIDKGLIPME GDEKLKMLAF DIETLYHEGE 150
EFAEGPILMI SYADEEGARV ITWKKVDLPY VDVVSTEKEM IKRFLRVVKE 200
KDPDVLITYN GDNFDFAYLK RRSEKLGVKF ILGRDGSEPK IQRMGDRFAV 250
EVKGRIHFDL YPVIRRTINL PTYTLEAVYE AIFGKPKEKV YAEEIATAWE 300
TGEGLERVAR YSMEDAKVTF ELGKEFFPME AQLSRLIGQS LWDVSRSSTG 350
NLVEWFLLRK AYERNELAPN KPDERELARR RQSYAGGYVK EPERGLWNNI 400
VYLDFRSLYP SIIITHNVSP DTLNREGCKE YDVAPQVGHK FCKDFPGFIP 450
SLLGDLLEER QKIKRKMRAT IDPVEKKLLD YRQRAIKILA NSILPDEWLP 500
LLVNGRLKLV RIGDFVDNTM KKGQPLENDG TEVLEVSGIE AISFNRKTKI 550
AEIKPVKALI RHRYRGKVYD IKLSSGRNIK VTEGHSLFAF RDGELVEVTG 600
GEIKPGDFIA VPRRVNLPER HERINLIEIL LGLPPEETSD IVLTIPVKGR 650
KNFFKGMLRT LRWIFEEEQR PRTARRYLEH LQKLGYVKLM KRAYEIVNKE 700
ALRNYRKLYE VLAERVKYNG NKREYLVHFN DLRNEIKFMP DEELEEWKVG 750
TLNGFRMEPF IEVGEDFAKL LGYYVSEGYA RKQRNQKNGW SYSVKIYNND 800
QRVLDDMEKL ASKFFGRVRR GKNYVEISRK MAYVLFESLC GTLAENKRVP 850
EVIFTSPESV RWAFFEGYFI GDGDLHPSKR VRLSTKSEEL VNGLVVLLNS 900
LGISAIKIRF DSGVYRVLVN EELPFLGNRK RKNAYYSHVI PKEILEETFG 950
KQFQKNMSPA KLNEKVEKGE LDAGKARRIA WLLEGDIVLD RVEKVTVEDY 1000
EGYVYDLSVE ENENFLAGFG MLYAHNSYYG YYGYAKARWY CRECAESVTA 1050
WGRSYIETTI REIEEKFGFK VLYADSVAGN TEVIIRRNGK VEFVPIEKLF 1100
QRVDYRIGEK EYCALEGVEA LTLDNRGRLV WRKVPYIMRH KTNKKIYRVW 1150
FTNSWYLDVT EDHSLIGYLN TSKVKSEKPL KERLVEVKPR ELGEKVKSLI 1200
TLNRAIARSI KANPIAVRLW ELIGLLVGDG NWGGHSKWAK YYVGLSCGLD 1250
KAEIEEKVLR PLKEAGIISN YYGKSKKGDV SILSKWLAGF MVKYFKDENG 1300
NKRIPSFMFN LPREYIEAFL RGLFSADGTV SLRRGIPEIR LTSVNRELSN 1350
EVRKLLWLVG VSNSMFTETT PNKYLGNESG TRSIHVRIKN KHRFAKRIGF 1400
LLDRKATKLS DNLREHTNKK MAYRYDFDLV YPKKIEEINY DRYVYDIEVE 1450
GTHRFFANGI LVHNTDGFFA TIPGADAETV KKKAMEFLKY INAKLPGLLE 1500
LEYEGFYVRG FFVTKKKYAV IDEEGKITTR GLEIVRRDWS EIAKETQARV 1550
LEAILKHGDV EEAVRIVKEV TEKLSKYEVP PEKLVIHEQI TRDLKDYKAT 1600
GPHVAVAKRL AARGIKIRPG TVISYIVLKG SGRIGDRAIP FDEFDPAKHK 1650
YDAEYYIENQ VLPAVERILR AFGYRKEDLR YQKTKQVGLG AWLKVKGKK 1699
Length:1,699
Mass (Da):197,325
Last modified:March 1, 2001 - v1
Checksum:iF389B4351F0B12D3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ250333 Genomic DNA. Translation: CAC12850.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ250333 Genomic DNA. Translation: CAC12850.1 .

3D structure databases

ProteinModelPortali Q9HH84.
SMRi Q9HH84. Positions 1-1076, 1456-1696.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.170.16.10. 4 hits.
3.10.28.10. 3 hits.
3.30.420.10. 1 hit.
InterProi IPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view ]
Pfami PF00136. DNA_pol_B. 3 hits.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14528. LAGLIDADG_3. 2 hits.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
SM00486. POLBc. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 4 hits.
SSF53098. SSF53098. 1 hit.
SSF55608. SSF55608. 2 hits.
TIGRFAMsi TIGR01443. intein_Cterm. 2 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEi PS50818. INTEIN_C_TER. 2 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Thermococcales taxonomy and phylogeny based on the comparative use of 16S rDNA, 16S-23S rDNA intergenic spacer and family B DNA polymerase genes."
    Querellou J.J.E., Cambon M.A., Lesongeur F., Barbier G.
    Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiDPOL_THEG8
AccessioniPrimary (citable) accession number: Q9HH84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 1, 2001
Last modified: September 3, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi