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Q9HH84 (DPOL_THEG8) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA polymerase

EC=2.7.7.7

Cleaved into the following 2 chains:

  1. Endonuclease PI-TspGE8I
    EC=3.1.-.-
    Alternative name(s):
    Tsp-GE8 pol-1 intein
  2. Endonuclease PI-TspGE8II
    EC=3.1.-.-
    Alternative name(s):
    Tsp-GE8 pol-2 intein
Gene names
Name:pol
Synonyms:pol-1
OrganismThermococcus sp. (strain GE8)
Taxonomic identifier105583 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length1699 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity By similarity.

PI-TspGE8I and PI-TspGE8II are endonucleases Potential.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Sequence similarities

Belongs to the DNA polymerase type-B family.

Contains 2 DOD-type homing endonuclease domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491DNA polymerase, 1st part
PRO_0000007343
Chain492 – 1026535Endonuclease PI-TspGE8I
PRO_0000007344
Chain1027 – 107549DNA polymerase, 2nd part
PRO_0000007345
Chain1076 – 1464389Endonuclease PI-TspGE8II
PRO_0000007346
Chain1465 – 1699235DNA polymerase, 3rd part
PRO_0000007347

Regions

Domain770 – 903134DOD-type homing endonuclease 1
Domain1222 – 1361140DOD-type homing endonuclease 2

Sequences

Sequence LengthMass (Da)Tools
Q9HH84 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: F389B4351F0B12D3

FASTA1,699197,325
        10         20         30         40         50         60 
MILDTDYITE DGKPVIRVFK KENGEFKIEY DRNFEPYFYA LLKDDSAIEE VKKITAKRHG 

        70         80         90        100        110        120 
TVVKVKRAEK VKKKFLGRPI EVWKLYFTHP QDVPAIRDKI REHPAVIDIY EYDIPFAKRY 

       130        140        150        160        170        180 
LIDKGLIPME GDEKLKMLAF DIETLYHEGE EFAEGPILMI SYADEEGARV ITWKKVDLPY 

       190        200        210        220        230        240 
VDVVSTEKEM IKRFLRVVKE KDPDVLITYN GDNFDFAYLK RRSEKLGVKF ILGRDGSEPK 

       250        260        270        280        290        300 
IQRMGDRFAV EVKGRIHFDL YPVIRRTINL PTYTLEAVYE AIFGKPKEKV YAEEIATAWE 

       310        320        330        340        350        360 
TGEGLERVAR YSMEDAKVTF ELGKEFFPME AQLSRLIGQS LWDVSRSSTG NLVEWFLLRK 

       370        380        390        400        410        420 
AYERNELAPN KPDERELARR RQSYAGGYVK EPERGLWNNI VYLDFRSLYP SIIITHNVSP 

       430        440        450        460        470        480 
DTLNREGCKE YDVAPQVGHK FCKDFPGFIP SLLGDLLEER QKIKRKMRAT IDPVEKKLLD 

       490        500        510        520        530        540 
YRQRAIKILA NSILPDEWLP LLVNGRLKLV RIGDFVDNTM KKGQPLENDG TEVLEVSGIE 

       550        560        570        580        590        600 
AISFNRKTKI AEIKPVKALI RHRYRGKVYD IKLSSGRNIK VTEGHSLFAF RDGELVEVTG 

       610        620        630        640        650        660 
GEIKPGDFIA VPRRVNLPER HERINLIEIL LGLPPEETSD IVLTIPVKGR KNFFKGMLRT 

       670        680        690        700        710        720 
LRWIFEEEQR PRTARRYLEH LQKLGYVKLM KRAYEIVNKE ALRNYRKLYE VLAERVKYNG 

       730        740        750        760        770        780 
NKREYLVHFN DLRNEIKFMP DEELEEWKVG TLNGFRMEPF IEVGEDFAKL LGYYVSEGYA 

       790        800        810        820        830        840 
RKQRNQKNGW SYSVKIYNND QRVLDDMEKL ASKFFGRVRR GKNYVEISRK MAYVLFESLC 

       850        860        870        880        890        900 
GTLAENKRVP EVIFTSPESV RWAFFEGYFI GDGDLHPSKR VRLSTKSEEL VNGLVVLLNS 

       910        920        930        940        950        960 
LGISAIKIRF DSGVYRVLVN EELPFLGNRK RKNAYYSHVI PKEILEETFG KQFQKNMSPA 

       970        980        990       1000       1010       1020 
KLNEKVEKGE LDAGKARRIA WLLEGDIVLD RVEKVTVEDY EGYVYDLSVE ENENFLAGFG 

      1030       1040       1050       1060       1070       1080 
MLYAHNSYYG YYGYAKARWY CRECAESVTA WGRSYIETTI REIEEKFGFK VLYADSVAGN 

      1090       1100       1110       1120       1130       1140 
TEVIIRRNGK VEFVPIEKLF QRVDYRIGEK EYCALEGVEA LTLDNRGRLV WRKVPYIMRH 

      1150       1160       1170       1180       1190       1200 
KTNKKIYRVW FTNSWYLDVT EDHSLIGYLN TSKVKSEKPL KERLVEVKPR ELGEKVKSLI 

      1210       1220       1230       1240       1250       1260 
TLNRAIARSI KANPIAVRLW ELIGLLVGDG NWGGHSKWAK YYVGLSCGLD KAEIEEKVLR 

      1270       1280       1290       1300       1310       1320 
PLKEAGIISN YYGKSKKGDV SILSKWLAGF MVKYFKDENG NKRIPSFMFN LPREYIEAFL 

      1330       1340       1350       1360       1370       1380 
RGLFSADGTV SLRRGIPEIR LTSVNRELSN EVRKLLWLVG VSNSMFTETT PNKYLGNESG 

      1390       1400       1410       1420       1430       1440 
TRSIHVRIKN KHRFAKRIGF LLDRKATKLS DNLREHTNKK MAYRYDFDLV YPKKIEEINY 

      1450       1460       1470       1480       1490       1500 
DRYVYDIEVE GTHRFFANGI LVHNTDGFFA TIPGADAETV KKKAMEFLKY INAKLPGLLE 

      1510       1520       1530       1540       1550       1560 
LEYEGFYVRG FFVTKKKYAV IDEEGKITTR GLEIVRRDWS EIAKETQARV LEAILKHGDV 

      1570       1580       1590       1600       1610       1620 
EEAVRIVKEV TEKLSKYEVP PEKLVIHEQI TRDLKDYKAT GPHVAVAKRL AARGIKIRPG 

      1630       1640       1650       1660       1670       1680 
TVISYIVLKG SGRIGDRAIP FDEFDPAKHK YDAEYYIENQ VLPAVERILR AFGYRKEDLR 

      1690 
YQKTKQVGLG AWLKVKGKK 

« Hide

References

[1]"Thermococcales taxonomy and phylogeny based on the comparative use of 16S rDNA, 16S-23S rDNA intergenic spacer and family B DNA polymerase genes."
Querellou J.J.E., Cambon M.A., Lesongeur F., Barbier G.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ250333 Genomic DNA. Translation: CAC12850.1.

3D structure databases

ProteinModelPortalQ9HH84.
SMRQ9HH84. Positions 1-1076, 1456-1696.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.170.16.10. 4 hits.
3.10.28.10. 3 hits.
3.30.420.10. 1 hit.
InterProIPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR004578. DNA-dir_DNA_pol_B_pol2.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamPF00136. DNA_pol_B. 3 hits.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14528. LAGLIDADG_3. 2 hits.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
SM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMSSF51294. SSF51294. 4 hits.
SSF53098. SSF53098. 1 hit.
SSF55608. SSF55608. 2 hits.
TIGRFAMsTIGR01443. intein_Cterm. 2 hits.
TIGR01445. intein_Nterm. 2 hits.
TIGR00592. pol2. 2 hits.
PROSITEPS50818. INTEIN_C_TER. 2 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDPOL_THEG8
AccessionPrimary (citable) accession number: Q9HH84
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 1, 2001
Last modified: June 11, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries