true2001-10-182024-03-27122GLNA_SULACThe glutamine synthetase from the hyperthermoacidophilic crenarcheon Sulfolobus acidocaldarius: isolation, characterization and sequencing of the gene.Yin Z.M.Purschke W.G.Schaefer G.Schmidt C.L.doi:10.1515/bchm.1998.379.11.13491998Biol. Chem.3791349-1354NUCLEOTIDE SEQUENCE [GENOMIC DNA]PROTEIN SEQUENCE OF 2-26FUNCTIONCATALYTIC ACTIVITYBIOPHYSICOCHEMICAL PROPERTIESACTIVITY REGULATIONCOFACTORSUBCELLULAR LOCATIONSUBUNITATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota.Chen L.Bruegger K.Skovgaard M.Redder P.She Q.Torarinsson E.Greve B.Awayez M.Zibat A.Klenk H.-P.Garrett R.A.doi:10.1128/jb.187.14.4992-4999.20052005J. Bacteriol.1874992-4999NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]Archaea6160Glutamine synthetase, N-terminal domainGlutamine synthetase/guanido kinase, catalytic domainGln_synt_NGln_synt_N_sfGln_synth/guanido_kin_cat_domGln_synth_cat_domGln_synth_gly_rich_siteGln_synth_IGln_synth_N_conserv_siteGlnAGLUTAMINE SYNTHETASELENGSINGln-synt_CGln-synt_NGln-synt_CGlutamine synthetase, N-terminal domainGlutamine synthetase/guanido kinaseGLNA_1GLNA_ATPGS_BETA_GRASPGS_CATALYTICGlutamine synthetaseGS6.3.1.2Glutamate--ammonia ligaseGlutamine synthetase I alphaGSI alphaglnASaci_1483Probably involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.Binds 2 Mg(2+) or Mn(2+) ions per subunit.Strongly inhibited by glycine and L-alanine. AMP at 10 mM displays a very weak inhibitory effect. The activity of this enzyme is not controlled by adenylation.0.15 mM for ADP0.24 mM for manganese1.3 mM for L-glutamineOptimum pH is between 7 and 7.5.Optimum temperature is 90 degrees Celsius. In the absence of magnesium or manganese ions about 50% of the activity is lost within 100 minutes at 78 degrees Celsius, whereas more than 95% of the activity is retained in presence of 4 mM manganese ions. Magnesium ions are a less effective.Oligomer of 12 subunits arranged in the form of two hexagons.GlnA of S.acidocaldarius is unique among the archeal glutamine synthetase since the regulatory properties suggest a position within the GS I-alpha subgroup. However, the sequence shows more pronounced similarities to the GS I-beta subgroup.Belongs to the glutamine synthetase family.Removed1Glutamine synthetase534552473GS beta-grasp18102GS catalytic110133135207212220264265269271273324330342347357362364V185TIEA204H209EVATAGQGDIDFRFSTLADTADKVQVLKYVTKNIASKRGMIATFMPKPF211259GDNGSGMHTHFSLWTKDGKNLMYDPNDEYAELSQIGRYI261299GPL301303EHGRALSAIV305314G317122005-08-30253586138001f3dadf80fbf2fa810d740bb130MPGLPKNEHEALEFLKSNNIKWVDLQFTDLLGKLQHITIPSNEFDESSFKVGFGKLDGSSIKGFTSIYESDMVLLPIPQTMTLIPWMQGVARVLTKVFWGGGKGRFERDPRGIAEEAEKYQSEQGYVSYFGPELEFFVFDKVEVDASLPQSGTGYKIHSREAPWSKNGGYVIRYKEGYYPASPVDQLMDIRLEIISTLVDYFGFTIEAAHHEVATAGQGEIDFRFSTLADTADKVQVLKYVTKNIASKRGMIATFMPKPFFGDNGSGMHTHFSLWTKDGKNLMYDPNDEYAELSQIGRYIIGGLLEHGRALSAIVAPTTNSYRRLVPGYEAPVYLVWSKSNRSAAIRIPAYYKGMEKAKRLEYRPPDPSSNPYLVFSAILMAGLDGIRRKLDPGDPVDENIYHMSEEKKRSLKIRELPGSLDEALNELESDNEFLKPVFNSSILQAYLDLKKEEAKMMQLYPHPMEIYQYLDStruetruetruetruetruetruetruetruetruetrue