Q9HH07 (DNLI_THEFM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase
EC=6.5.1.1

Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:

lig

Organism

Thermococcus fumicolans

Taxonomic identifier

46540 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity. HAMAP MF_00407
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	DNA ligase HAMAP MF_00407		PRO_0000059621
Sites
	Active site	249	1	N6-AMP-lysine intermediate By similarity
	Binding site	247	1	ATP By similarity
	Binding site	254	1	ATP By similarity
	Binding site	269	1	ATP By similarity
	Binding site	299	1	ATP By similarity
	Binding site	339	1	ATP By similarity
	Binding site	414	1	ATP By similarity
	Binding site	420	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HH07 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 173DCFF6FC0110BB	FASTA	559	63,598
	10 20 30 40 50 60 MKYSELAGLY RRLEKTTLKT LKTRFVADFL KNVPDELLEI VPYLILGKVF PDWDERELGV 70 80 90 100 110 120 GEKLLIKAVS IATGVPEGEI ENSIKDTGDL GESIALAVKK KKQKSFFSQP LTIKRVYDTF 130 140 150 160 170 180 VKVAESQGEG SQDRKMKYLA NLFMDAQPEE AKYIARTVLG TMRTGVAEGI LRDAIAEAFK 190 200 210 220 230 240 VKAELVERAY MLTSDFGYVT KVAKLEGNEG LSKVRIQVGK PVRPMLAQNA ASVKDALLEM 250 260 270 280 290 300 GGEAAFEIKY DGARVQVHKD GDRVVIYSRR LENVTRSIPE IVEAVRSQLR PEKAIVEGEL 310 320 330 340 350 360 VAVGDGGKPR PFQYVLRRFR RKYNIEEMIE RIPLELNLFD VLYVDGESLV DTPFMERRKR 370 380 390 400 410 420 LEEAVEESER IKLAQQLVTK KAEEAEEFYR RALELGHEGL MAKRLDSVYE PGNRGKKWLK 430 440 450 460 470 480 IKPTMEDLDL VIIGAEWGEG RRAHLLGSFL VAAYDQHRGE FVPVGKVGSG FTDEDLAEFT 490 500 510 520 530 540 KMLKPLIVRE EGKYVEIEPR VVIQVTYQEI QKSPKYESGF ALRFPRYVAL REDKSPEEAD 550 TIERISELYG LQERFKAKR

References

[1]	"Characterization of a thermophilic DNA ligase from the archaeon Thermococcus fumicolans." Rolland J.-L., Gueguen Y., Persillon C., Masson J.-M., Dietrich J. FEMS Microbiol. Lett. 236:267-273(2004) [PubMed: 15251207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ133713 Genomic DNA. Translation: CAC21199.1.
3D structure databases
ProteinModelPortal	Q9HH07.
SMR	Q9HH07. Positions 1-558.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	6.5.1.1. 6299.
Family and domain databases
HAMAP	MF_00407. DNA_ligase. [Tree]
InterPro	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:1.10.3260.10. DNA_ligase_ATP-dep_N. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. Dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_THEFM

Accession

Primary (citable) accession number: Q9HH07

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	March 1, 2001
Last modified:	May 31, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program