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Protein

Squalene synthase

Gene

ERG9

Organism
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. Required for the biosynthesis of ergosterol.

Catalytic activityi

2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)+.

Cofactori

Mg2+By similarity

Pathwayi: lanosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Squalene synthase (ERG9)
  2. Squalene monooxygenase (ERG1)
  3. no protein annotated in this organism
This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.

GO - Molecular functioni

  • farnesyl-diphosphate farnesyltransferase activity Source: CGD
  • squalene synthase activity Source: CGD

GO - Biological processi

  • ergosterol biosynthetic process Source: CGD
  • isoprenoid biosynthetic process Source: UniProtKB-KW

Keywordsi

Molecular functionMultifunctional enzyme, Transferase
Biological processIsoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandMagnesium, NADP

Enzyme and pathway databases

UniPathwayiUPA00767; UER00751

Names & Taxonomyi

Protein namesi
Recommended name:
Squalene synthase (EC:2.5.1.21)
Short name:
SQS
Short name:
SS
Alternative name(s):
FPP:FPP farnesyltransferase
Farnesyl-diphosphate farnesyltransferase
Gene namesi
Name:ERG9
Ordered Locus Names:CAGL0M07095g
OrganismiCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Taxonomic identifieri284593 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNakaseomycesNakaseomyces/Candida clade
Proteomesi
  • UP000002428 Componenti: Chromosome M

Organism-specific databases

CGDiCAL0136937 ERG9
EuPathDBiFungiDB:CAGL0M07095g

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei181 – 201HelicalSequence analysisAdd BLAST21
Transmembranei294 – 314HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000674481 – 443Squalene synthaseAdd BLAST443

Interactioni

Protein-protein interaction databases

STRINGi284593.XP_449656.1

Structurei

3D structure databases

ProteinModelPortaliQ9HGZ6
SMRiQ9HGZ6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1459 Eukaryota
COG1562 LUCA
HOGENOMiHOG000186940
InParanoidiQ9HGZ6
KOiK00801
OMAiFNFVAIP
OrthoDBiEOG092C2IU6

Family and domain databases

CDDicd00683 Trans_IPPS_HH, 1 hit
Gene3Di1.10.600.10, 1 hit
InterProiView protein in InterPro
IPR008949 Isoprenoid_synthase_dom_sf
IPR002060 Squ/phyt_synthse
IPR006449 Squal_synth-like
IPR019845 Squalene/phytoene_synthase_CS
IPR033904 Trans_IPPS_HH
PfamiView protein in Pfam
PF00494 SQS_PSY, 1 hit
SUPFAMiSSF48576 SSF48576, 1 hit
TIGRFAMsiTIGR01559 squal_synth, 1 hit
PROSITEiView protein in PROSITE
PS01044 SQUALEN_PHYTOEN_SYN_1, 1 hit
PS01045 SQUALEN_PHYTOEN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HGZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKVLDLALH PLELRAALKL KFIRQPLFST NDTRATPQLE RCYELLNLTS
60 70 80 90 100
RSFAAVIMEL HPELRNVIMV FYLILRALDT VEDDMTIDPQ LKVKVLREFD
110 120 130 140 150
SKLDTTDWSF DGNDLKEKDR VVLTEFPCIL GEYHKLKPEY QKVIKRITGL
160 170 180 190 200
MGNGMADYIL DENFNLNGVQ TVKDYDKYCH YVAGLVGDGL TELIVLAGFG
210 220 230 240 250
SDDLYHGKNS FQLYESMGLF LQKTNIIRDY AEDLDDGRSF WPKEIWSEYA
260 270 280 290 300
TKLTDFRDPK NTQKGVDCIN HLVLNALTHV IDVLTYLSSI HEQSSFQFCA
310 320 330 340 350
IPQVMAIATL AKVFNNPEVL RKNVKIRKGT TCDLILNSRT LKGCVDIFQY
360 370 380 390 400
YLRDMKQRLP VEDPNYLKFN IQVAKIEQFI EEMFQDNLPA GVEPRETMIY
410 420 430 440
LKVQERLKWD TQVIPRVQEE DYKFNMALSV VFCVLLSFYF FTK
Length:443
Mass (Da):51,397
Last modified:August 31, 2004 - v2
Checksum:iC23BC9D603E44C9F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti217M → L in BAB12207 (PubMed:10952588).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009978 Genomic DNA Translation: BAB12207.1
CR380959 Genomic DNA Translation: CAG62632.1
RefSeqiXP_449656.1, XM_449656.1

Genome annotation databases

EnsemblFungiiCAG62632; CAG62632; CAGL0M07095g
GeneIDi2891705
KEGGicgr:CAGL0M07095g

Similar proteinsi

Entry informationi

Entry nameiFDFT_CANGA
AccessioniPrimary (citable) accession number: Q9HGZ6
Secondary accession number(s): Q6FJD8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: August 31, 2004
Last modified: April 25, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health