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Q9HGZ1 (K6PF1_ASPOR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phosphofructokinase subunit alpha

EC=2.7.1.11
Alternative name(s):
6PF-1-K subunit alpha
Phosphofructokinase 1
Phosphohexokinase
Gene names
Name:pfkA
ORF Names:AO090003001390
OrganismAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) [Complete proteome]
Taxonomic identifier510516 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length785 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339

Enzyme regulation

Allosterically inhibited by ATP and activated by AMP and fructose 2,6-bisphosphate By similarity. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339

Subunit structure

Heterooctamer of 4 alpha and 4 beta chains By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   DomainRepeat
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termAllosteric enzyme
Complete proteome
Gene Ontology (GO)
   Biological_processfructose 6-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolysis

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_component6-phosphofructokinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phosphofructokinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7857856-phosphofructokinase subunit alpha HAMAP-Rule MF_00339
PRO_0000112036

Regions

Nucleotide binding33 – 375ATP By similarity
Nucleotide binding191 – 1955ATP By similarity
Nucleotide binding208 – 22417ATP By similarity

Sites

Active site1641Proton acceptor By similarity
Metal binding2221Magnesium; via carbonyl oxygen By similarity
Binding site1991Substrate By similarity
Binding site2911Substrate By similarity
Binding site2971Substrate By similarity
Binding site3001Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9HGZ1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: B6BD92CBFF928954

FASTA78585,970
        10         20         30         40         50         60 
MATTHAPAEP PKRRRIGVLT SGGDAPGMNG AVRAVVRMAI YSGCEAYAVY EGYEGLVHGG 

        70         80         90        100        110        120 
DMIRQVHWED VRGWLSRGGT LIGSARSMAF RERAGRLKAA KNMIVRGIDA LVVCGGDGSL 

       130        140        150        160        170        180 
TGADLFRSEW PGLLEELVKT GELTEEQIVP YKVLNIVGLV GSIDNDMSGT DATIGCYSSL 

       190        200        210        220        230        240 
TRICDAVDDV FDTAYSHQRG FVIEVMGRHC GWLALMSAIS TGADWLFIPE MPPREGWEDD 

       250        260        270        280        290        300 
MCDIITKNRQ ERGKRRTIVI VAEGAHDRQL NKITSSKIKD ILTNRLDLDT RVTVLGHTQR 

       310        320        330        340        350        360 
GGAACAYDRT LSTLQGVEAV RAVLDMTPES PSPVITVREN KLLRTPLMDA VKATKEVADL 

       370        380        390        400        410        420 
IHERKFDEAM HLRDSEFKEY HFAYKNTATP DHPKLILPEN KRMRIAIIHV GAPAGGMNQA 

       430        440        450        460        470        480 
TRAAVAYCQT RGHTALAVHN GFPGLCRHHA DTPVSSVREV SWEEQDTWVN EGGSDIGTNR 

       490        500        510        520        530        540 
SLPSEDFETT AKCFEKFKFD GLFVVGGFEA FTAVSQLRQA REKYPAFKIP MVVLPATISN 

       550        560        570        580        590        600 
NVPGTEYSLG SDTCLNTLID FCDAIRQSAS SSRRRVFVVE TQGGKSGYVA TTAGLAVGAS 

       610        620        630        640        650        660 
AVYIPEEGID IKMLARDIDF LRNNFAHDKG ANRAGKIILR NETASSTYTT QVIADMIKEE 

       670        680        690        700        710        720 
AKGRFESRAA VPGHFQQGGK PSPMDRIRAL RMAIRCMQHI ETFSGKSADE IAADELSATV 

       730        740        750        760        770        780 
IGVKGSQVLF SQMGGPNGLE ATETDWARRR PKDEFWLDLQ STVNILSGRA SFGEGKTGWS 


CYENC 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB032270 mRNA. Translation: BAB12230.1.
AP007155 Genomic DNA. Translation: BAE58448.1.
RefSeqXP_001820450.2. XM_001820398.2.

3D structure databases

ProteinModelPortalQ9HGZ1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADAORAT00001912; CADAORAP00001882; CADAORAG00001912.
GeneID5992433.
KEGGaor:AOR_1_2444154.

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000200154.
KOK00850.
OMAVYHMASK.
OrthoDBEOG7Q5HPV.

Enzyme and pathway databases

UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase.
InterProIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 2 hits.
[Graphical view]
PIRSFPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 2 hits.
TIGRFAMsTIGR02478. 6PF1K_euk. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameK6PF1_ASPOR
AccessionPrimary (citable) accession number: Q9HGZ1
Secondary accession number(s): Q2UJ17
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways