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Q9HGZ1

- PFKA1_ASPOR

UniProt

Q9HGZ1 - PFKA1_ASPOR

Protein

ATP-dependent 6-phosphofructokinase 1

Gene

pfkA

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei23 – 231ATP; via amide nitrogenUniRule annotation
    Metal bindingi117 – 1171Magnesium; catalyticUniRule annotation
    Active sitei164 – 1641Proton acceptorUniRule annotation
    Binding sitei199 – 1991Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei263 – 2631SubstrateUniRule annotation
    Binding sitei291 – 2911Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei480 – 4801Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei575 – 5751Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei642 – 6421Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei668 – 6681Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei749 – 7491Allosteric activator fructose 2,6-bisphosphateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi86 – 872ATPUniRule annotation
    Nucleotide bindingi116 – 1194ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase 1UniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFK 1UniRule annotation
    Short name:
    Phosphofructokinase 1UniRule annotation
    Alternative name(s):
    Phosphohexokinase 1UniRule annotation
    Gene namesi
    Name:pfkA
    ORF Names:AO090003001390
    OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
    Taxonomic identifieri510516 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000006564: Chromosome 2

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 785785ATP-dependent 6-phosphofructokinase 1PRO_0000112036Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HGZ1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 389389N-terminal catalytic PFK domain 1Add
    BLAST
    Regioni162 – 1643Substrate bindingUniRule annotation
    Regioni206 – 2083Substrate bindingUniRule annotation
    Regioni297 – 3004Substrate bindingUniRule annotation
    Regioni390 – 40314Interdomain linkerAdd
    BLAST
    Regioni404 – 785382C-terminal regulatory PFK domain 2Add
    BLAST
    Regioni537 – 5415Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni582 – 5843Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni674 – 6774Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000200154.
    KOiK00850.
    OMAiVQHGITN.
    OrthoDBiEOG7Q5HPV.

    Family and domain databases

    HAMAPiMF_03184. Phosphofructokinase_I_E.
    InterProiIPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 2 hits.
    TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HGZ1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATTHAPAEP PKRRRIGVLT SGGDAPGMNG AVRAVVRMAI YSGCEAYAVY    50
    EGYEGLVHGG DMIRQVHWED VRGWLSRGGT LIGSARSMAF RERAGRLKAA 100
    KNMIVRGIDA LVVCGGDGSL TGADLFRSEW PGLLEELVKT GELTEEQIVP 150
    YKVLNIVGLV GSIDNDMSGT DATIGCYSSL TRICDAVDDV FDTAYSHQRG 200
    FVIEVMGRHC GWLALMSAIS TGADWLFIPE MPPREGWEDD MCDIITKNRQ 250
    ERGKRRTIVI VAEGAHDRQL NKITSSKIKD ILTNRLDLDT RVTVLGHTQR 300
    GGAACAYDRT LSTLQGVEAV RAVLDMTPES PSPVITVREN KLLRTPLMDA 350
    VKATKEVADL IHERKFDEAM HLRDSEFKEY HFAYKNTATP DHPKLILPEN 400
    KRMRIAIIHV GAPAGGMNQA TRAAVAYCQT RGHTALAVHN GFPGLCRHHA 450
    DTPVSSVREV SWEEQDTWVN EGGSDIGTNR SLPSEDFETT AKCFEKFKFD 500
    GLFVVGGFEA FTAVSQLRQA REKYPAFKIP MVVLPATISN NVPGTEYSLG 550
    SDTCLNTLID FCDAIRQSAS SSRRRVFVVE TQGGKSGYVA TTAGLAVGAS 600
    AVYIPEEGID IKMLARDIDF LRNNFAHDKG ANRAGKIILR NETASSTYTT 650
    QVIADMIKEE AKGRFESRAA VPGHFQQGGK PSPMDRIRAL RMAIRCMQHI 700
    ETFSGKSADE IAADELSATV IGVKGSQVLF SQMGGPNGLE ATETDWARRR 750
    PKDEFWLDLQ STVNILSGRA SFGEGKTGWS CYENC 785
    Length:785
    Mass (Da):85,970
    Last modified:March 1, 2001 - v1
    Checksum:iB6BD92CBFF928954
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB032270 mRNA. Translation: BAB12230.1.
    AP007155 Genomic DNA. Translation: BAE58448.1.
    RefSeqiXP_001820450.2. XM_001820398.2.

    Genome annotation databases

    EnsemblFungiiCADAORAT00001912; CADAORAP00001882; CADAORAG00001912.
    GeneIDi5992433.
    KEGGiaor:AOR_1_2444154.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB032270 mRNA. Translation: BAB12230.1 .
    AP007155 Genomic DNA. Translation: BAE58448.1 .
    RefSeqi XP_001820450.2. XM_001820398.2.

    3D structure databases

    ProteinModelPortali Q9HGZ1.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADAORAT00001912 ; CADAORAP00001882 ; CADAORAG00001912 .
    GeneIDi 5992433.
    KEGGi aor:AOR_1_2444154.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000200154.
    KOi K00850.
    OMAi VQHGITN.
    OrthoDBi EOG7Q5HPV.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .

    Family and domain databases

    HAMAPi MF_03184. Phosphofructokinase_I_E.
    InterProi IPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 2 hits.
    TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of glycolytic gene from Aspergillus oryzae."
      Nakajima K., Kunihiro S., Sano M., Eto S., Machida M.
      Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ATCC 42149 / RIB 40.
    2. "Genome sequencing and analysis of Aspergillus oryzae."
      Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
      , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
      Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 42149 / RIB 40.

    Entry informationi

    Entry nameiPFKA1_ASPOR
    AccessioniPrimary (citable) accession number: Q9HGZ1
    Secondary accession number(s): Q2UJ17
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2003
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3