SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9HGZ1

- PFKA1_ASPOR

UniProt

Q9HGZ1 - PFKA1_ASPOR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
ATP-dependent 6-phosphofructokinase 1
Gene
pfkA, AO090003001390
Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei23 – 231ATP; via amide nitrogen By similarity
Metal bindingi117 – 1171Magnesium; catalytic By similarity
Active sitei164 – 1641Proton acceptor By similarity
Binding sitei199 – 1991Substrate; shared with dimeric partner By similarity
Binding sitei263 – 2631Substrate By similarity
Binding sitei291 – 2911Substrate; shared with dimeric partner By similarity
Binding sitei480 – 4801Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei575 – 5751Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei642 – 6421Allosteric activator fructose 2,6-bisphosphate By similarity
Binding sitei668 – 6681Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partner By similarity
Binding sitei749 – 7491Allosteric activator fructose 2,6-bisphosphate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi86 – 872ATP By similarity
Nucleotide bindingi116 – 1194ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 1 (EC:2.7.1.11)
Short name:
ATP-PFK 1
Short name:
Phosphofructokinase 1
Alternative name(s):
Phosphohexokinase 1
Gene namesi
Name:pfkA
ORF Names:AO090003001390
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000006564: Chromosome 2

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 785785ATP-dependent 6-phosphofructokinase 1UniRule annotation
PRO_0000112036Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ9HGZ1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 389389N-terminal catalytic PFK domain 1UniRule annotation
Add
BLAST
Regioni162 – 1643Substrate binding By similarity
Regioni206 – 2083Substrate binding By similarity
Regioni297 – 3004Substrate binding By similarity
Regioni390 – 40314Interdomain linkerUniRule annotation
Add
BLAST
Regioni404 – 785382C-terminal regulatory PFK domain 2UniRule annotation
Add
BLAST
Regioni537 – 5415Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni582 – 5843Allosteric activator fructose 2,6-bisphosphate binding By similarity
Regioni674 – 6774Allosteric activator fructose 2,6-bisphosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000200154.
KOiK00850.
OMAiVQHGITN.
OrthoDBiEOG7Q5HPV.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HGZ1-1 [UniParc]FASTAAdd to Basket

« Hide

MATTHAPAEP PKRRRIGVLT SGGDAPGMNG AVRAVVRMAI YSGCEAYAVY    50
EGYEGLVHGG DMIRQVHWED VRGWLSRGGT LIGSARSMAF RERAGRLKAA 100
KNMIVRGIDA LVVCGGDGSL TGADLFRSEW PGLLEELVKT GELTEEQIVP 150
YKVLNIVGLV GSIDNDMSGT DATIGCYSSL TRICDAVDDV FDTAYSHQRG 200
FVIEVMGRHC GWLALMSAIS TGADWLFIPE MPPREGWEDD MCDIITKNRQ 250
ERGKRRTIVI VAEGAHDRQL NKITSSKIKD ILTNRLDLDT RVTVLGHTQR 300
GGAACAYDRT LSTLQGVEAV RAVLDMTPES PSPVITVREN KLLRTPLMDA 350
VKATKEVADL IHERKFDEAM HLRDSEFKEY HFAYKNTATP DHPKLILPEN 400
KRMRIAIIHV GAPAGGMNQA TRAAVAYCQT RGHTALAVHN GFPGLCRHHA 450
DTPVSSVREV SWEEQDTWVN EGGSDIGTNR SLPSEDFETT AKCFEKFKFD 500
GLFVVGGFEA FTAVSQLRQA REKYPAFKIP MVVLPATISN NVPGTEYSLG 550
SDTCLNTLID FCDAIRQSAS SSRRRVFVVE TQGGKSGYVA TTAGLAVGAS 600
AVYIPEEGID IKMLARDIDF LRNNFAHDKG ANRAGKIILR NETASSTYTT 650
QVIADMIKEE AKGRFESRAA VPGHFQQGGK PSPMDRIRAL RMAIRCMQHI 700
ETFSGKSADE IAADELSATV IGVKGSQVLF SQMGGPNGLE ATETDWARRR 750
PKDEFWLDLQ STVNILSGRA SFGEGKTGWS CYENC 785
Length:785
Mass (Da):85,970
Last modified:March 1, 2001 - v1
Checksum:iB6BD92CBFF928954
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB032270 mRNA. Translation: BAB12230.1.
AP007155 Genomic DNA. Translation: BAE58448.1.
RefSeqiXP_001820450.2. XM_001820398.2.

Genome annotation databases

EnsemblFungiiCADAORAT00001912; CADAORAP00001882; CADAORAG00001912.
GeneIDi5992433.
KEGGiaor:AOR_1_2444154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB032270 mRNA. Translation: BAB12230.1 .
AP007155 Genomic DNA. Translation: BAE58448.1 .
RefSeqi XP_001820450.2. XM_001820398.2.

3D structure databases

ProteinModelPortali Q9HGZ1.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADAORAT00001912 ; CADAORAP00001882 ; CADAORAG00001912 .
GeneIDi 5992433.
KEGGi aor:AOR_1_2444154.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000200154.
KOi K00850.
OMAi VQHGITN.
OrthoDBi EOG7Q5HPV.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .

Family and domain databases

HAMAPi MF_03184. Phosphofructokinase_I_E.
InterProi IPR009161. 6-phosphofructokinase_euk.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 2 hits.
[Graphical view ]
PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 2 hits.
TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of glycolytic gene from Aspergillus oryzae."
    Nakajima K., Kunihiro S., Sano M., Eto S., Machida M.
    Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ATCC 42149 / RIB 40.
  2. "Genome sequencing and analysis of Aspergillus oryzae."
    Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
    , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
    Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 42149 / RIB 40.

Entry informationi

Entry nameiPFKA1_ASPOR
AccessioniPrimary (citable) accession number: Q9HGZ1
Secondary accession number(s): Q2UJ17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi