6-phosphofructokinase subunit alpha - Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)

Preferred order of sections

Names and origin

Protein names

Recommended name:
6-phosphofructokinase subunit alpha
EC=2.7.1.11

Alternative name(s):
6PF-1-K subunit alpha
Phosphofructokinase 1
Phosphohexokinase

Gene names

Name:	pfkA
ORF Names:	AO090003001390

Organism

Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) [Complete proteome]

Taxonomic identifier

510516 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus ›

Protein attributes

Sequence length	785 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Enzyme regulation	Allosterically inhibited by ATP and activated by AMP and fructose 2,6-bisphosphate By similarity.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Heterooctamer of 4 alpha and 4 beta chains By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 785

785

6-phosphofructokinase subunit alpha

PRO_0000112036

Regions

Nucleotide binding

33 – 37

5

ATP By similarity

Nucleotide binding

191 – 195

5

ATP By similarity

Nucleotide binding

208 – 224

17

ATP By similarity

Sites

Active site

164

1

Proton acceptor By similarity

Metal binding

222

1

Magnesium; via carbonyl oxygen By similarity

Binding site

199

1

Substrate By similarity

Binding site

291

1

Substrate By similarity

Binding site

297

1

Substrate By similarity

Binding site

300

1

Substrate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q9HGZ1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: B6BD92CBFF928954
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FASTA

785

85,970

        10         20         30         40         50         60 
MATTHAPAEP PKRRRIGVLT SGGDAPGMNG AVRAVVRMAI YSGCEAYAVY EGYEGLVHGG 

        70         80         90        100        110        120 
DMIRQVHWED VRGWLSRGGT LIGSARSMAF RERAGRLKAA KNMIVRGIDA LVVCGGDGSL 

       130        140        150        160        170        180 
TGADLFRSEW PGLLEELVKT GELTEEQIVP YKVLNIVGLV GSIDNDMSGT DATIGCYSSL 

       190        200        210        220        230        240 
TRICDAVDDV FDTAYSHQRG FVIEVMGRHC GWLALMSAIS TGADWLFIPE MPPREGWEDD 

       250        260        270        280        290        300 
MCDIITKNRQ ERGKRRTIVI VAEGAHDRQL NKITSSKIKD ILTNRLDLDT RVTVLGHTQR 

       310        320        330        340        350        360 
GGAACAYDRT LSTLQGVEAV RAVLDMTPES PSPVITVREN KLLRTPLMDA VKATKEVADL 

       370        380        390        400        410        420 
IHERKFDEAM HLRDSEFKEY HFAYKNTATP DHPKLILPEN KRMRIAIIHV GAPAGGMNQA 

       430        440        450        460        470        480 
TRAAVAYCQT RGHTALAVHN GFPGLCRHHA DTPVSSVREV SWEEQDTWVN EGGSDIGTNR 

       490        500        510        520        530        540 
SLPSEDFETT AKCFEKFKFD GLFVVGGFEA FTAVSQLRQA REKYPAFKIP MVVLPATISN 

       550        560        570        580        590        600 
NVPGTEYSLG SDTCLNTLID FCDAIRQSAS SSRRRVFVVE TQGGKSGYVA TTAGLAVGAS 

       610        620        630        640        650        660 
AVYIPEEGID IKMLARDIDF LRNNFAHDKG ANRAGKIILR NETASSTYTT QVIADMIKEE 

       670        680        690        700        710        720 
AKGRFESRAA VPGHFQQGGK PSPMDRIRAL RMAIRCMQHI ETFSGKSADE IAADELSATV 

       730        740        750        760        770        780 
IGVKGSQVLF SQMGGPNGLE ATETDWARRR PKDEFWLDLQ STVNILSGRA SFGEGKTGWS 


CYENC

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of glycolytic gene from Aspergillus oryzae." Nakajima K., Kunihiro S., Sano M., Eto S., Machida M. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 42149 / RIB 40.
[2]	"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. Kikuchi H. Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB032270 mRNA. Translation: BAB12230.1. AP007155 Genomic DNA. Translation: BAE58448.1.
RefSeq	XP_001820450.2. XM_001820398.2.
3D structure databases
ProteinModelPortal	Q9HGZ1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAORAT00001912; CADAORAP00001882; CADAORAG00001912.
GeneID	5992433.
KEGG	aor:AOR_1_2444154.
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000200154.
KO	K00850.
OrthoDB	EOG418FWH.
Enzyme and pathway databases
UniPathway	UPA00109; UER00182.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 2 hits.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF1_ASPOR

Accession

Primary (citable) accession number: Q9HGZ1
Secondary accession number(s): Q2UJ17

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents