Reviewed, UniProtKB/Swiss-Prot Q9HGZ1 (K6PF1_ASPOR)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase subunit alpha
    EC=2.7.1.11
Alternative name(s):
    Phosphofructokinase 1
    Phosphohexokinase
    6PF-1-K subunit alpha

Gene names

Name:	pfkA
ORF Names:	AO090003001390

Organism

Aspergillus oryzae [Complete proteome]

Taxonomic identifier

5062 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	785 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Enzyme regulation	Allosterically inhibited by ATP and activated by AMP and fructose 2,6-bisphosphate By similarity.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Heterooctamer of 4 alpha and 4 beta chains By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 785	785	6-phosphofructokinase subunit alpha		PRO_0000112036
Regions
	Nucleotide binding	33 – 37	5	ATP By similarity
	Nucleotide binding	191 – 195	5	ATP By similarity
	Nucleotide binding	208 – 224	17	ATP By similarity
Sites
	Active site	164	1	Proton acceptor By similarity
	Metal binding	222	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	199	1	Substrate By similarity
	Binding site	291	1	Substrate By similarity
	Binding site	297	1	Substrate By similarity
	Binding site	300	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HGZ1-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: B6BD92CBFF928954	FASTA	785	85,970
	10 20 30 40 50 60 MATTHAPAEP PKRRRIGVLT SGGDAPGMNG AVRAVVRMAI YSGCEAYAVY EGYEGLVHGG 70 80 90 100 110 120 DMIRQVHWED VRGWLSRGGT LIGSARSMAF RERAGRLKAA KNMIVRGIDA LVVCGGDGSL 130 140 150 160 170 180 TGADLFRSEW PGLLEELVKT GELTEEQIVP YKVLNIVGLV GSIDNDMSGT DATIGCYSSL 190 200 210 220 230 240 TRICDAVDDV FDTAYSHQRG FVIEVMGRHC GWLALMSAIS TGADWLFIPE MPPREGWEDD 250 260 270 280 290 300 MCDIITKNRQ ERGKRRTIVI VAEGAHDRQL NKITSSKIKD ILTNRLDLDT RVTVLGHTQR 310 320 330 340 350 360 GGAACAYDRT LSTLQGVEAV RAVLDMTPES PSPVITVREN KLLRTPLMDA VKATKEVADL 370 380 390 400 410 420 IHERKFDEAM HLRDSEFKEY HFAYKNTATP DHPKLILPEN KRMRIAIIHV GAPAGGMNQA 430 440 450 460 470 480 TRAAVAYCQT RGHTALAVHN GFPGLCRHHA DTPVSSVREV SWEEQDTWVN EGGSDIGTNR 490 500 510 520 530 540 SLPSEDFETT AKCFEKFKFD GLFVVGGFEA FTAVSQLRQA REKYPAFKIP MVVLPATISN 550 560 570 580 590 600 NVPGTEYSLG SDTCLNTLID FCDAIRQSAS SSRRRVFVVE TQGGKSGYVA TTAGLAVGAS 610 620 630 640 650 660 AVYIPEEGID IKMLARDIDF LRNNFAHDKG ANRAGKIILR NETASSTYTT QVIADMIKEE 670 680 690 700 710 720 AKGRFESRAA VPGHFQQGGK PSPMDRIRAL RMAIRCMQHI ETFSGKSADE IAADELSATV 730 740 750 760 770 780 IGVKGSQVLF SQMGGPNGLE ATETDWARRR PKDEFWLDLQ STVNILSGRA SFGEGKTGWS CYENC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of glycolytic gene from Aspergillus oryzae." Nakajima K., Kunihiro S., Sano M., Eto S., Machida M. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 42149 / RIB 40.
[2]	"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H. Nature 438:1157-1161(2005) [PubMed: 16372010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB032270 mRNA. Translation: BAB12230.1. AP007155 Genomic DNA. Translation: BAE58448.1.
RefSeq	XP_001820450.1.
3D structure databases
HSSP	HSSP built from PDB template 3PFK based on UniProtKB P00512.
ModBase	Search...
Genome annotation databases
GeneID	5992433.
GenomeReviews	Gene locus pfkA in contig AP007155_GR.
KEGG	aor:AO090003001390.
Enzyme and pathway databases
BRENDA	2.7.1.11. 2240.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
ProDom	PD000707. Ppfruckinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF1_ASPOR

Accession

Primary (citable) accession number: Q9HGZ1
Secondary accession number(s): Q2UJ17

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	March 1, 2001
Last modified:	September 22, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)