6-phosphofructokinase subunit beta - Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)

Preferred order of sections

Names and origin

Protein names

Recommended name:
6-phosphofructokinase subunit beta
EC=2.7.1.11

Alternative name(s):
6PF-1-K subunit beta
Phosphofructokinase 2
Phosphohexokinase

Gene names

Name:	pfkB
ORF Names:	AO090010000444

Organism

Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) [Complete proteome]

Taxonomic identifier

510516 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	775 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.
Enzyme regulation	Allosterically inhibited by ATP and activated by AMP and fructose 2,6-bisphosphate By similarity.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit structure	Heterooctamer of 4 alpha and 4 beta chains By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family. Two domains subfamily.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 775

775

6-phosphofructokinase subunit beta

PRO_0000112037

Regions

Nucleotide binding

35 – 39

5

ATP By similarity

Nucleotide binding

193 – 197

5

ATP By similarity

Nucleotide binding

210 – 226

17

ATP By similarity

Sites

Active site

166

1

Proton acceptor By similarity

Metal binding

224

1

Magnesium; via carbonyl oxygen By similarity

Binding site

201

1

Substrate By similarity

Binding site

292

1

Substrate By similarity

Binding site

298

1

Substrate By similarity

Binding site

301

1

Substrate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q9HGZ0 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: B5A78C4F7EE43848
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FASTA

775

84,727

        10         20         30         40         50         60 
MTNTILDTYS SRRKPRRIGI LTSGGDAPGM NGAIRAVVRT AIQNGCEAWA IHEGYEGLIQ 

        70         80         90        100        110        120 
GGAMMHPLYW EDVRGFLSRG GTLIGSVRCD RFREREGRLQ AARNMVLFGI DALVVCGGDG 

       130        140        150        160        170        180 
SLTGADLFRS EWPELLNELV STGVLTVAQV APHQNLNIVG LLGSIDNDFS GTDATIGCYS 

       190        200        210        220        230        240 
ALTRICEAVD AVFDTASSHR RGFVVEVMGR HCGWLALMAA IATGADWLFI PERPPRDGWE 

       250        260        270        280        290        300 
DDMCSIITKN RNRGKRRTIV ILAEGAQDSN LDRISSSAVK DVLSKRLGLD TRVTVLGHIQ 

       310        320        330        340        350        360 
RGGSPCAYDR WLSTLQGIHA VKAVLSMTPE SPSPVVIIQE NRIRTSSLAE TVALTKEANA 

       370        380        390        400        410        420 
SMHAKEFEKA ATLRDPEFME YHSAYRHLNT SDHPKMVLPE DKRMRVAIIH VGAPAAGMNP 

       430        440        450        460        470        480 
ATRAVVAYCL TRGHTPIAIH NGFPGLCRHH DDTPGSVREM HWLESGDWIN DGGSDIGTNA 

       490        500        510        520        530        540 
GLPLDDIETT AQCFERYKFD ALFVIGGFEA FTAVSQLRKA RKQYLAFRIP LVLLPASMSN 

       550        560        570        580        590        600 
NVPGTEYSLG SDTSLNTLVY FCDVVRQSAS SSGHSVFVVE AQGAEYQATA AALAAGAMTV 

       610        620        630        640        650        660 
YTPERGITLQ SLSNDIEYLR QQFSKDHGAN RSGKLIIRND QTSTIYSTTE IANIIKHEAK 

       670        680        690        700        710        720 
NRFDAQGVVP GHFQQGGKVS PIDRIRAFRL AVKCMEHLET FAGQSPEEIM NDENSATVIS 

       730        740        750        760        770 
IKQSRILLLP MGGPTGVEAT DTDWKRQRPK TQNWLEIQEA VDSLSGRSSL YAIPN

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of glycolytic gene from Aspergillus oryzae." Nakajima K., Kunihiro S., Sano M., Eto S., Machida M. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 42149 / RIB 40.
[2]	"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. Kikuchi H. Nature 438:1157-1161(2005) [PubMed: 16372010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB032271 mRNA. Translation: BAB12231.1. AP007175 Genomic DNA. Translation: BAE66301.1.
RefSeq	XP_001827434.1. XM_001827382.2.
3D structure databases
ProteinModelPortal	Q9HGZ0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9HGZ0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAORAT00004509; CADAORAP00004429; CADAORAG00004509.
GeneID	5999568.
GenomeReviews	Gene locus pfkB in contig AP007175_GR.
KEGG	aor:AOR_1_724024.
Phylogenomic databases
GeneTree	EFGT00050000004244.
OrthoDB	EOG44N231.
Family and domain databases
InterPro	IPR009161. 6-phosphofructokinase_euk. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
KO	K00850.
Pfam	PF00365. PFK. 2 hits. [Graphical view]
PIRSF	PIRSF000533. ATP_PFK_euk. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 2 hits.
TIGRFAMs	TIGR02478. 6PF1K_euk. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF2_ASPOR

Accession

Primary (citable) accession number: Q9HGZ0
Secondary accession number(s): Q2TWS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents