Reviewed, UniProtKB/Swiss-Prot Q9HGY2 (GPD1_ZYGRO)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD+] 1
    EC=1.1.1.8
Alternative name(s):
    ZrGPD1

Gene names

Name:

GPD1

Organism

Zygosaccharomyces rouxii (Candida mogii)

Taxonomic identifier

4956 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Zygosaccharomyces

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Glycerol-3-phosphate dehydrogenase [NAD+] 1		PRO_0000138101
Regions
	Nucleotide binding	40 – 45	6	NAD By similarity
	Region	309 – 310	2	Substrate binding By similarity
Sites
	Active site	244	1	Proton acceptor By similarity
	Binding site	128	1	NAD By similarity
	Binding site	151	1	NAD; via amide nitrogen By similarity
	Binding site	151	1	Substrate By similarity
	Binding site	184	1	NAD; via amide nitrogen By similarity
	Binding site	309	1	NAD By similarity
	Binding site	338	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HGY2-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: E698797A2AA20A16	FASTA	401	43,791
	10 20 30 40 50 60 MAATDRLNQT SDILSQSMKK TDSSMSVVTA ENPYKVSVVG SGNWGTTIAK VVAENTKEKP 70 80 90 100 110 120 ELFQERVDMW VFEEQIDGTP LAQIINTKHQ NVKYLPNIDL PDNLVANPDL IATTKDADVI 130 140 150 160 170 180 VFNVPHQFLG RIVAQMKGQI KPTARAVSCL KGFEVGPKGV QLLSDYVTQE LGIECGALSG 190 200 210 220 230 240 ANLAPEVAKE HWSETTVAYH IPDDFKGDGK DIDHRVLKQL FHRPYFHVNV IDDVAGISIA 250 260 270 280 290 300 GALKNVVALG CGFVTGLGWG NNAAAAIQRV GLGEIIKFGR MFFPESKVET YYQESAGVAD 310 320 330 340 350 360 LITTCSGGRN VRVATEMAKT GKSGEQVEKD ILNGQSAQGL VTCKEVHQWL ESSGNTEDFP 370 380 390 400 LFEAVYQITY ENVPMKELPS MIEELDIDST SKCVLSYKMG L

References

[1]

"Cloning of glycerol-3-phosphate dehydrogenase genes (ZrGPD1 and ZrGPD2) and glycerol dehydrogenase genes (ZrGCY1 and ZrGCY2) from the salt-tolerant yeast Zygosaccharomyces rouxii." Iwaki T., Kurono S., Yokose Y., Kubota K., Tamai Y., Watanabe Y. Yeast 18:737-744(2001) [PubMed: 11378901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 42981.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB047394 Genomic DNA. Translation: BAB11957.1.
3D structure databases
HSSP	HSSP built from PDB template 1EVY based on UniProtKB P90551.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.8. 266875.
Family and domain databases
InterPro	IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. IPR006168. NAD-dep_Gly3P_DH. IPR017751. NAD-dep_Gly3P_DH_euk. IPR011128. NAD-dep_Gly3P_DH_N. IPR006109. NAD_Gly3P_DH_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR11728. NAD_Gly3P_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTS	PR00077. GPDHDRGNASE.
ProDom	PD001278. NAD_Gly3P_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03376. glycerol3P_DH. 1 hit.
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPD1_ZYGRO

Accession

Primary (citable) accession number: Q9HGY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)