Reviewed, UniProtKB/Swiss-Prot Q9HGY1 (GPD2_ZYGRO)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD+] 2
    EC=1.1.1.8
Alternative name(s):
    ZrGPD2

Gene names

Name:

GPD2

Organism

Zygosaccharomyces rouxii (Candida mogii)

Taxonomic identifier

4956 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Zygosaccharomyces

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC protein homodimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Glycerol-3-phosphate dehydrogenase [NAD+] 2		PRO_0000138102
Regions
	Nucleotide binding	40 – 45	6	NAD By similarity
	Region	309 – 310	2	Substrate binding By similarity
Sites
	Active site	244	1	Proton acceptor By similarity
	Binding site	128	1	NAD By similarity
	Binding site	151	1	NAD; via amide nitrogen By similarity
	Binding site	151	1	Substrate By similarity
	Binding site	184	1	NAD; via amide nitrogen By similarity
	Binding site	309	1	NAD By similarity
	Binding site	338	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HGY1-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 70BBA9C2AAB463D0	FASTA	389	42,511
	10 20 30 40 50 60 MAATDRLNQT SDILSHSMKK TDTSMSIVTA ENPYKVAVVG SGNWGTTIAK VVAENTKEKP 70 80 90 100 110 120 ELFQGRVDMW VFEEQIDGTP LTQIINTKHQ NVKYLPNIDL PGNLVANPDL ISTTKDADVI 130 140 150 160 170 180 VFNVPHQFLG RIVSQMKGQI KPDARAISCL KGFEVGPKGV QLLSDYVTQE LGIQCGALSG 190 200 210 220 230 240 ANLAPEVAKE HWSETTVAYQ VPDDFKGEGK DIDHRVLKQL FHRPYFHVNV IDDVAGISIA 250 260 270 280 290 300 GALKNVVALG CGFVTGLGWG NNAAAAIQRV GLGEIIKFGR MFFPESKVET YYQESAGVAD 310 320 330 340 350 360 LITTCSGGRN VRVATEMAKT GKSGEQVEKD ILNGQSAQGL ITAKEVHQWL ESSGHTEEYP 370 380 LFEAVYQITY ENVPMKELPS MIEELDIVE

References

[1]

"Cloning of glycerol-3-phosphate dehydrogenase genes (ZrGPD1 and ZrGPD2) and glycerol dehydrogenase genes (ZrGCY1 and ZrGCY2) from the salt-tolerant yeast Zygosaccharomyces rouxii." Iwaki T., Kurono S., Yokose Y., Kubota K., Tamai Y., Watanabe Y. Yeast 18:737-744(2001) [PubMed: 11378901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 42981.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB047395 mRNA. Translation: BAB11958.1.
3D structure databases
HSSP	HSSP built from PDB template 1EVY based on UniProtKB P90551.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.8. 266875.
Family and domain databases
InterPro	IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. IPR006168. NAD-dep_Gly3P_DH. IPR017751. NAD-dep_Gly3P_DH_euk. IPR011128. NAD-dep_Gly3P_DH_N. IPR006109. NAD_Gly3P_DH_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR11728. NAD_Gly3P_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTS	PR00077. GPDHDRGNASE.
ProDom	PD001278. NAD_Gly3P_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03376. glycerol3P_DH. 1 hit.
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPD2_ZYGRO

Accession

Primary (citable) accession number: Q9HGY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)