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Protein

Serine--tRNA ligase, cytoplasmic

Gene

SES1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).1 Publication

Catalytic activityi

ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser).
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).

Pathwayi: selenocysteinyl-tRNA(Sec) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec).
Proteins known to be involved in this subpathway in this organism are:
  1. Serine--tRNA ligase, cytoplasmic (SES1)
This subpathway is part of the pathway selenocysteinyl-tRNA(Sec) biosynthesis, which is itself part of Aminoacyl-tRNA biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-seryl-tRNA(Sec) from L-serine and tRNA(Sec), the pathway selenocysteinyl-tRNA(Sec) biosynthesis and in Aminoacyl-tRNA biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei295ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei302SerineBy similarity1
Binding sitei404SerineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi279 – 281ATPBy similarity3
Nucleotide bindingi366 – 369ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.11. 1096.
UniPathwayiUPA00906; UER00895.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine--tRNA ligase, cytoplasmic (EC:6.1.1.11)
Alternative name(s):
Seryl-tRNA synthetase
Short name:
SerRS
Seryl-tRNA(Ser/Sec) synthetase
Gene namesi
Name:SES1
ORF Names:CaO19.7901
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001221971 – 462Serine--tRNA ligase, cytoplasmicAdd BLAST462

Proteomic databases

PRIDEiQ9HGT6.

2D gel databases

COMPLUYEAST-2DPAGEQ9HGT6.

Interactioni

Subunit structurei

Homodimer. The tRNA molecule binds across the dimer (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-59702N.

Structurei

Secondary structure

1462
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 7Combined sources4
Helixi9 – 11Combined sources3
Helixi15 – 25Combined sources11
Helixi30 – 68Combined sources39
Helixi74 – 105Combined sources32
Helixi122 – 124Combined sources3
Beta strandi126 – 131Combined sources6
Beta strandi138 – 142Combined sources5
Turni144 – 146Combined sources3
Helixi154 – 160Combined sources7
Helixi166 – 173Combined sources8
Helixi183 – 200Combined sources18
Turni201 – 203Combined sources3
Beta strandi205 – 208Combined sources4
Beta strandi211 – 214Combined sources4
Helixi215 – 221Combined sources7
Helixi224 – 227Combined sources4
Turni228 – 230Combined sources3
Beta strandi233 – 236Combined sources4
Beta strandi239 – 243Combined sources5
Helixi248 – 254Combined sources7
Turni255 – 257Combined sources3
Beta strandi259 – 262Combined sources4
Helixi263 – 266Combined sources4
Beta strandi269 – 278Combined sources10
Beta strandi291 – 294Combined sources4
Beta strandi296 – 307Combined sources12
Helixi309 – 311Combined sources3
Helixi312 – 329Combined sources18
Beta strandi334 – 338Combined sources5
Helixi341 – 343Combined sources3
Beta strandi349 – 358Combined sources10
Turni359 – 362Combined sources4
Beta strandi363 – 372Combined sources10
Helixi376 – 380Combined sources5
Beta strandi399 – 407Combined sources9
Helixi408 – 418Combined sources11
Beta strandi419 – 421Combined sources3
Beta strandi424 – 426Combined sources3
Helixi429 – 434Combined sources6
Beta strandi440 – 443Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QNEX-ray2.00A1-462[»]
3QO5X-ray2.30A1-462[»]
3QO7X-ray2.55A1-462[»]
3QO8X-ray2.00A1-462[»]
ProteinModelPortaliQ9HGT6.
SMRiQ9HGT6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HGT6.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni246 – 248Serine bindingBy similarity3

Domaini

Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.By similarity

Sequence similaritiesi

Phylogenomic databases

InParanoidiQ9HGT6.
KOiK01875.
OrthoDBiEOG092C513S.

Family and domain databases

CDDicd00770. SerRS_core. 1 hit.
Gene3Di1.10.287.40. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-ligase_type_1.
IPR015866. Ser-tRNA-synth_1_N.
IPR033729. SerRS_core.
IPR010978. tRNA-bd_arm.
[Graphical view]
PANTHERiPTHR11778. PTHR11778. 1 hit.
PfamiPF02403. Seryl_tRNA_N. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PIRSFiPIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTSiPR00981. TRNASYNTHSER.
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00414. serS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HGT6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDINAFLVE KGGDPEIIKA SQKKRGDSVE LVDEIIAEYK EWVKLRFDLD
60 70 80 90 100
EHNKKLNSVQ KEIGKRFKAK EDAKDLIAEK EKLSNEKKEI IEKEAEADKN
110 120 130 140 150
LRSKINQVGN IVHESVVDSQ DEENNELVRT WTPENYKKPE QIAAATGAPA
160 170 180 190 200
KLSHHEVLLR LDGYDPERGV RIVGHRGYFL RNYGVFLNQA LINYGLSFLS
210 220 230 240 250
SKGYVPLQAP VMMNKEVMAK TAQLSQFDEE LYKVIDGEDE KYLIATSEQP
260 270 280 290 300
ISAYHAGEWF ESPAEQLPVR YAGYSSCFRR EAGSHGKDAW GIFRVHAFEK
310 320 330 340 350
IEQFVLTEPE KSWEEFDRMI GCSEEFYQSL GLPYRVVGIV SGELNNAAAK
360 370 380 390 400
KYDLEAWFPF QQEYKELVSC SNCTDYQSRN LEIRCGIKQQ NQQEKKYVHC
410 420 430 440 450
LNSTLSATER TICCILENYQ KEDGLVIPEV LRKYIPGEPE FIPYIKELPK
460
NTTSVKKAKG KN
Length:462
Mass (Da):52,991
Last modified:March 1, 2001 - v1
Checksum:i9111C72CF7B37CDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290915 Genomic DNA. Translation: AAG02209.1.
AACQ01000026 Genomic DNA. Translation: EAL01244.1.
RefSeqiXP_720099.1. XM_715006.1.

Genome annotation databases

EnsemblFungiiEAL01244; EAL01244; CaO19.7901.
GeneIDi3638226.
KEGGical:CaO19.7901.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290915 Genomic DNA. Translation: AAG02209.1.
AACQ01000026 Genomic DNA. Translation: EAL01244.1.
RefSeqiXP_720099.1. XM_715006.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QNEX-ray2.00A1-462[»]
3QO5X-ray2.30A1-462[»]
3QO7X-ray2.55A1-462[»]
3QO8X-ray2.00A1-462[»]
ProteinModelPortaliQ9HGT6.
SMRiQ9HGT6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59702N.

2D gel databases

COMPLUYEAST-2DPAGEQ9HGT6.

Proteomic databases

PRIDEiQ9HGT6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAL01244; EAL01244; CaO19.7901.
GeneIDi3638226.
KEGGical:CaO19.7901.

Phylogenomic databases

InParanoidiQ9HGT6.
KOiK01875.
OrthoDBiEOG092C513S.

Enzyme and pathway databases

UniPathwayiUPA00906; UER00895.
BRENDAi6.1.1.11. 1096.

Miscellaneous databases

EvolutionaryTraceiQ9HGT6.

Family and domain databases

CDDicd00770. SerRS_core. 1 hit.
Gene3Di1.10.287.40. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-ligase_type_1.
IPR015866. Ser-tRNA-synth_1_N.
IPR033729. SerRS_core.
IPR010978. tRNA-bd_arm.
[Graphical view]
PANTHERiPTHR11778. PTHR11778. 1 hit.
PfamiPF02403. Seryl_tRNA_N. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PIRSFiPIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTSiPR00981. TRNASYNTHSER.
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00414. serS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYSC_CANAL
AccessioniPrimary (citable) accession number: Q9HGT6
Secondary accession number(s): Q5AEY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Candida albicans
    Candida albicans: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.