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Q9HGN6 (DUS1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]
EC=1.3.1.88
Alternative name(s):
tRNA-dihydrouridine synthase 1
Gene names
Name:dus1
ORF Names:SPBC36B7.04
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length399 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs By similarity.

Catalytic activity

5,6-dihydrouracil(16) in tRNA + NAD(P)+ = uracil(16) in tRNA + NAD(P)H.

5,6-dihydrouracil(17) in tRNA + NAD(P)+ = uracil(17) in tRNA + NAD(P)H.

Cofactor

FAD By similarity.

Subcellular location

Nucleus. Mitochondrion Ref.2.

Sequence similarities

Belongs to the dus family. Dus1 subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentMitochondrion
Nucleus
   LigandFAD
Flavoprotein
NAD
NADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processtRNA modification

Inferred from sequence or structural similarity. Source: GeneDB_Spombe

   Cellular componentmitochondrion

Inferred from direct assay. Source: GeneDB_Spombe

nucleus

Inferred from direct assay. Source: GeneDB_Spombe

   Molecular functionflavin adenine dinucleotide binding

Inferred from electronic annotation. Source: InterPro

tRNA dihydrouridine synthase activity

Inferred from sequence or structural similarity. Source: GeneDB_Spombe

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 399399tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]
PRO_0000316225

Sequences

Sequence LengthMass (Da)Tools
Q9HGN6 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 79713175C803AD56

FASTA39945,327
        10         20         30         40         50         60 
MASKKLHGRD FYNKIGRPKR ILAPMVDQSE LPWRILARRS GADLCYSPMF HSRLFGESED 

        70         80         90        100        110        120 
YRNKVFSTRT IPEERPLIIQ FCGNDPEIML KAAKIAAPYC DAVDVNLGCP QGIAKKGKYG 

       130        140        150        160        170        180 
SFLQENWNLI ESIITKLHTE LSIPVTAKIR IFPDPQKTLD YAKMILKAGA SILAVHGRLR 

       190        200        210        220        230        240 
EQKGHFTGIA DWEQIQMLRK NLPSETVLFA NGNILHAQDI DRCIKYTGVD GVLSAEGSLY 

       250        260        270        280        290        300 
NPRIFLPPSS PLMTLYPRID DMCEEYLNII REFKLESDYS SLSAIKGHLF KLMRPLLSIH 

       310        320        330        340        350        360 
TDIRSKLAQG CTPRDFETFP PVVAMLRKRL LECEEKGEIN EDKDVKESVK DSMGYPVIPW 

       370        380        390 
WRVQPYIRPL EVPLVTKRKP VEVTADEGPV KKKVNASVA

« Hide

References

[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU329671 Genomic DNA. Translation: CAC05725.1.
RefSeqNP_595986.1. NM_001021893.1.

3D structure databases

ProteinModelPortalQ9HGN6.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9HGN6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC36B7.04.1; SPBC36B7.04.1:pep; SPBC36B7.04.
GeneID2541048.
GenomeReviewsGene locus dus1 in contig CU329671_GR.
KEGGspo:SPBC36B7.04.
NMPDRfig|4896.1.peg.1852.

Organism-specific databases

GeneDB_SpombeSPBC36B7.04.

Phylogenomic databases

eggNOGfuNOG04299.
GeneTreeEFGT00050000005657.
HOGENOMHBG629132.
OMAPYWRCQP.
OrthoDBEOG480N5B.

Gene expression databases

ArrayExpressQ9HGN6.

Family and domain databases

InterProIPR013785. Aldolase_TIM.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK05542.
PANTHERPTHR11082. Du_synth. 1 hit.
PfamPF01207. Dus. 1 hit.
[Graphical view]
PROSITEPS01136. UPF0034. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDUS1_SCHPO
AccessionPrimary (citable) accession number: Q9HGN6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 1, 2001
Last modified: January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents