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Protein

ATP-dependent RNA helicase dbp8

Gene

dbp8

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

ATP-binding RNA helicase involved in 40S ribosomal subunit biogenesis and is required for the normal formation of 18S rRNAs through pre-rRNA processing at A0, A1 and A2 sites. Required for vegetative growth (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi51 – 58ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processRibosome biogenesis, rRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase dbp8 (EC:3.6.4.13)
Gene namesi
Name:dbp8
ORF Names:SPBC543.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC543.06c
PomBaseiSPBC543.06c dbp8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002322911 – 453ATP-dependent RNA helicase dbp8Add BLAST453

Proteomic databases

MaxQBiQ9HGM5
PaxDbiQ9HGM5
PRIDEiQ9HGM5

Interactioni

Protein-protein interaction databases

STRINGi4896.SPBC543.06c.1

Structurei

3D structure databases

ProteinModelPortaliQ9HGM5
SMRiQ9HGM5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 214Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST177
Domaini230 – 393Helicase C-terminalPROSITE-ProRule annotationAdd BLAST164

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi7 – 35Q motifAdd BLAST29
Motifi160 – 163DEAD box4

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000268802
InParanoidiQ9HGM5
KOiK14778
OMAiPMKAEDY
OrthoDBiEOG092C1VLP
PhylomeDBiQ9HGM5

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HGM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEHTRKSFS DLGISPWLID TLKALAIYEP TDIQEGVIAQ ILEGRNCIGG
60 70 80 90 100
AKTGSGKTAA FALPIIEKWS KDPSGIFALI LTPTRELAIQ IDEQFAALGA
110 120 130 140 150
NLNLKHALIV GGMDMIRQSI DLSKRPHVVV ATPGRLADLI RSNGEETIAG
160 170 180 190 200
LRRIKFLVMD EADRLLSPTF ADDLDDCFSV LPASEDRQTL LFTATVTDAI
210 220 230 240 250
RQLKYQPQKN NKPPLWLYEV ETDNISVPST LQQSYIFVSS QVREAYLVHL
260 270 280 290 300
LTIPENAKKS AIIFVNRTRT AELIYSILRL LELRVTELHS EMVQRERINS
310 320 330 340 350
LGRFRAEAAK ILVATDVASR GLDIPSVQLV INFDLPRDPD DYIHRVGRTA
360 370 380 390 400
RAGRSGESIS IVTERDVDLV HAIEDRVGTK LSEYEHVSEN KMLEYIKEVT
410 420 430 440 450
DAKRQASLEM IDRGFGERRQ KRNEKRLMAN GISNKLKNSG RKKKAKNTLS

TEK
Length:453
Mass (Da):50,771
Last modified:March 1, 2001 - v1
Checksum:i7DBE88C5DBD45DC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAC05248.1
RefSeqiNP_596794.1, NM_001023814.2

Genome annotation databases

EnsemblFungiiSPBC543.06c.1; SPBC543.06c.1:pep; SPBC543.06c
GeneIDi2541069
KEGGispo:SPBC543.06c

Entry informationi

Entry nameiDBP8_SCHPO
AccessioniPrimary (citable) accession number: Q9HGM5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: March 1, 2001
Last modified: March 28, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health