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Protein

ATP-dependent RNA helicase dbp8

Gene

dbp8

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

ATP-binding RNA helicase involved in 40S ribosomal subunit biogenesis and is required for the normal formation of 18S rRNAs through pre-rRNA processing at A0, A1 and A2 sites. Required for vegetative growth (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi51 – 588ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-SPO-6791226. Major pathway of rRNA processing in the nucleolus.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase dbp8 (EC:3.6.4.13)
Gene namesi
Name:dbp8
ORF Names:SPBC543.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC543.06c.
PomBaseiSPBC543.06c. dbp8.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 453453ATP-dependent RNA helicase dbp8PRO_0000232291Add
BLAST

Proteomic databases

MaxQBiQ9HGM5.

Interactioni

Protein-protein interaction databases

MINTiMINT-4703257.

Structurei

3D structure databases

ProteinModelPortaliQ9HGM5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 214177Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini230 – 393164Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi7 – 3529Q motifAdd
BLAST
Motifi160 – 1634DEAD box

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000268802.
InParanoidiQ9HGM5.
KOiK14778.
OMAiNYDVPRN.
OrthoDBiEOG092C1VLP.
PhylomeDBiQ9HGM5.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HGM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEHTRKSFS DLGISPWLID TLKALAIYEP TDIQEGVIAQ ILEGRNCIGG
60 70 80 90 100
AKTGSGKTAA FALPIIEKWS KDPSGIFALI LTPTRELAIQ IDEQFAALGA
110 120 130 140 150
NLNLKHALIV GGMDMIRQSI DLSKRPHVVV ATPGRLADLI RSNGEETIAG
160 170 180 190 200
LRRIKFLVMD EADRLLSPTF ADDLDDCFSV LPASEDRQTL LFTATVTDAI
210 220 230 240 250
RQLKYQPQKN NKPPLWLYEV ETDNISVPST LQQSYIFVSS QVREAYLVHL
260 270 280 290 300
LTIPENAKKS AIIFVNRTRT AELIYSILRL LELRVTELHS EMVQRERINS
310 320 330 340 350
LGRFRAEAAK ILVATDVASR GLDIPSVQLV INFDLPRDPD DYIHRVGRTA
360 370 380 390 400
RAGRSGESIS IVTERDVDLV HAIEDRVGTK LSEYEHVSEN KMLEYIKEVT
410 420 430 440 450
DAKRQASLEM IDRGFGERRQ KRNEKRLMAN GISNKLKNSG RKKKAKNTLS

TEK
Length:453
Mass (Da):50,771
Last modified:March 1, 2001 - v1
Checksum:i7DBE88C5DBD45DC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAC05248.1.
RefSeqiNP_596794.1. NM_001023814.2.

Genome annotation databases

EnsemblFungiiSPBC543.06c.1; SPBC543.06c.1:pep; SPBC543.06c.
GeneIDi2541069.
KEGGispo:SPBC543.06c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAC05248.1.
RefSeqiNP_596794.1. NM_001023814.2.

3D structure databases

ProteinModelPortaliQ9HGM5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4703257.

Proteomic databases

MaxQBiQ9HGM5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC543.06c.1; SPBC543.06c.1:pep; SPBC543.06c.
GeneIDi2541069.
KEGGispo:SPBC543.06c.

Organism-specific databases

EuPathDBiFungiDB:SPBC543.06c.
PomBaseiSPBC543.06c. dbp8.

Phylogenomic databases

HOGENOMiHOG000268802.
InParanoidiQ9HGM5.
KOiK14778.
OMAiNYDVPRN.
OrthoDBiEOG092C1VLP.
PhylomeDBiQ9HGM5.

Enzyme and pathway databases

ReactomeiR-SPO-6791226. Major pathway of rRNA processing in the nucleolus.

Miscellaneous databases

PROiQ9HGM5.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDBP8_SCHPO
AccessioniPrimary (citable) accession number: Q9HGM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.