Q9HG01 (GRP78_PICAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
78 kDa glucose-regulated protein homolog
Short name=GRP-78

Alternative name(s):
Immunoglobulin heavy chain-binding protein homolog
Short name=BiP

Gene names

Name:

BiP

Organism

Pichia angusta (Yeast) (Hansenula polymorpha)

Taxonomic identifier

870730 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetales incertae sedis › Ogataea

Protein attributes

Sequence length	665 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Endoplasmic reticulum
Domain	Signal
Ligand	ATP-binding Nucleotide-binding
Gene Ontology (GO)
Biological_process	response to unfolded protein Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Chain	34 – 665	632	78 kDa glucose-regulated protein homolog		PRO_0000013584
Regions
	Motif	662 – 665	4	Prevents secretion from ER Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9HG01 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 9A23F7BCCC0364B7	FASTA	665	73,222
	10 20 30 40 50 60 MLTFNKSVVS CAAIIYALLL VVLPLTTQQF VKAESNENYG TVIGIDLGTT YSCVGVMKAG 70 80 90 100 110 120 RVEIIPNDQG NRITPSYVAF TEDERLVGDA AKNQIASNPT NTIFDIKRLI GHRFDDKVIQ 130 140 150 160 170 180 KEIKHLPYKV KDQDGRPVVE AKVNGELKTF TAEEISAMIL GKMKQIAEDY LGKKVTHAVV 190 200 210 220 230 240 TVPAYFNDAQ RQATKDAGTI AGLEVLRIVN EPTAAAIAYG LDKTDEEKHI IVYDLGGGTF 250 260 270 280 290 300 DVSLLTIAGG AFEVLATAGD THLGGEDFDY RVVRHFIKVF KKKHGIDISD NSKALAKLKR 310 320 330 340 350 360 EVEKAKRTLS SQMSTRIEID SFVDGIDFSE SLSRAKFEEL NMDLFKKTLK PVQQVLDDAK 370 380 390 400 410 420 MKPDEIDDVV FVGGSTRIPK VQELIENFFN GKKISKGINP DEAVAFGAAV QGGVLSGEEG 430 440 450 460 470 480 VEDIVLIDVN PLTLGIETSG GVMTTLIKRN TPIPTQKSQI FSTAADNQPV VLIQVYEGER 490 500 510 520 530 540 AMAKDNNLLG KFELTGIPPA PRGVPQIEVT FTLDSNGILK VSATDKGTGK SNSITITNDK 550 560 570 580 590 600 GRLSKEEIEK KIEEAEKFAQ QDKELREKVE SRNALENYAH SLKNQANDEN GFGAKLEEDD 610 620 630 640 650 660 KETLLDAINE ALEFLEDNFD TATKDEFDEQ KEKLSKVAYP ITSKLYDAPP TSDEEDEDDW DHDEL

References

[1]

"Overproduction of BiP negatively affects the secretion of Aspergillus niger glucose oxidase by the yeast Hansenula polymorpha." van der Heide M., Hollenberg C.P., van der Klei I.J., Veenhuis M. Appl. Microbiol. Biotechnol. 58:487-494(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 14754 / CBS 1976 / JCM 3620 / NBRC 0799 / NCYC 495 / NRRL Y-1798 / VKM Y-1397.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF245405 Genomic DNA. Translation: AAG09776.1.
3D structure databases
ProteinModelPortal	Q9HG01.
SMR	Q9HG01. Positions 40-579.
ModBase	Search...
Proteomic databases
PRIDE	Q9HG01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRP78_PICAN

Accession

Primary (citable) accession number: Q9HG01

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program