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Q9HFU4

- MPI_CRYNJ

UniProt

Q9HFU4 - MPI_CRYNJ

Protein

Mannose-6-phosphate isomerase

Gene

MAN1

Organism
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (21 Jun 2005)
      Previous versions | rss
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    Functioni

    Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.By similarity

    Catalytic activityi

    D-mannose 6-phosphate = D-fructose 6-phosphate.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi109 – 1091ZincBy similarity
    Metal bindingi111 – 1111ZincBy similarity
    Metal bindingi136 – 1361ZincBy similarity
    Metal bindingi291 – 2911ZincBy similarity
    Active sitei310 – 3101By similarity

    GO - Molecular functioni

    1. mannose-6-phosphate isomerase activity Source: UniProtKB-EC
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00126; UER00423.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannose-6-phosphate isomerase (EC:5.3.1.8)
    Alternative name(s):
    Phosphohexomutase
    Phosphomannose isomerase
    Short name:
    PMI
    Gene namesi
    Name:MAN1
    Ordered Locus Names:CNI02370
    OrganismiCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
    Taxonomic identifieri214684 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
    ProteomesiUP000002149: Chromosome 9

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 434434Mannose-6-phosphate isomerasePRO_0000194243Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi214684.CNI02370.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HFU4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1482.
    KOiK01809.
    OrthoDBiEOG7WDNCR.

    Family and domain databases

    Gene3Di2.60.120.10. 3 hits.
    InterProiIPR001250. Man6P_Isoase-1.
    IPR016305. Mannose-6-P_Isomerase.
    IPR018050. Pmannose_isomerase-type1_CS.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view]
    PANTHERiPTHR10309. PTHR10309. 1 hit.
    PfamiPF01238. PMI_typeI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
    PRINTSiPR00714. MAN6PISMRASE.
    SUPFAMiSSF51182. SSF51182. 1 hit.
    TIGRFAMsiTIGR00218. manA. 1 hit.
    PROSITEiPS00965. PMI_I_1. 1 hit.
    PS00966. PMI_I_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HFU4-1 [UniParc]FASTAAdd to Basket

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    MSPSVFKISP GINSYDWGKK GSASLAAQLA TTSIPDFSID EDKAYAELWM    50
    GTHPNNPSRL SDNTLLSEHL KSHPELIGSS VSSKFEDCKD GSLPFLFKVL 100
    SIGTALSIQA HPDKPLAKKL FDEKPDVYKD PNHKPEMAIA LTPFLAFLNF 150
    LPLSVLLLHL LTVPELQEFV DSSLTESLAS SLGLPTSQPP DTSLFKPTES 200
    PATAEQKDIL KQIFAALMSA DKKLVEEAIS KLIKRYQAKR DIKENEKSLV 250
    DLALRLNDQY PGDVGVLCVF LLNVVELKRG EAAFLGANEP HAYIEGDIIE 300
    CMATSDNVVR AGLTPKLRDV DTLVSMLTYE AAPGNKQLLQ PTPFQKGDDT 350
    TKLYDPPIAE FSVLRTELSK GMKTSHRPVE GPSLCVITEG EGVVRNGNDR 400
    SEFVRGDVIF VGAGKEVEWE AIKGLEMFRA YVEA 434
    Length:434
    Mass (Da):47,590
    Last modified:June 21, 2005 - v2
    Checksum:i4C2541EEFE9793FF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti38 – 381S → A in AAG10203. (PubMed:11359567)Curated
    Sequence conflicti43 – 431K → R in AAG10203. (PubMed:11359567)Curated
    Sequence conflicti71 – 711K → R in AAG10203. (PubMed:11359567)Curated
    Sequence conflicti79 – 802SS → TT in AAG10203. (PubMed:11359567)Curated
    Sequence conflicti154 – 1541S → P in AAG10203. (PubMed:11359567)Curated
    Sequence conflicti183 – 1831G → D in AAG10203. (PubMed:11359567)Curated
    Sequence conflicti192 – 1921T → S in AAG10203. (PubMed:11359567)Curated
    Sequence conflicti224 – 2241L → H in AAG10203. (PubMed:11359567)Curated
    Sequence conflicti237 – 2371Q → K in AAG10203. (PubMed:11359567)Curated
    Sequence conflicti248 – 2481S → D in AAG10203. (PubMed:11359567)Curated
    Sequence conflicti343 – 3431P → S in AAG10203. (PubMed:11359567)Curated
    Sequence conflicti396 – 3961N → D in AAG10203. (PubMed:11359567)Curated
    Sequence conflicti400 – 4012RS → QT in AAG10203. (PubMed:11359567)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF291701 Genomic DNA. Translation: AAG10203.1.
    AE017349 Genomic DNA. Translation: AAW45398.1.
    RefSeqiXP_572705.1. XM_572705.1.

    Genome annotation databases

    EnsemblFungiiAAW45398; AAW45398; CNI02370.
    GeneIDi3259465.
    KEGGicne:CNI02370.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF291701 Genomic DNA. Translation: AAG10203.1 .
    AE017349 Genomic DNA. Translation: AAW45398.1 .
    RefSeqi XP_572705.1. XM_572705.1.

    3D structure databases

    ProteinModelPortali Q9HFU4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 214684.CNI02370.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii AAW45398 ; AAW45398 ; CNI02370 .
    GeneIDi 3259465.
    KEGGi cne:CNI02370.

    Phylogenomic databases

    eggNOGi COG1482.
    KOi K01809.
    OrthoDBi EOG7WDNCR.

    Enzyme and pathway databases

    UniPathwayi UPA00126 ; UER00423 .

    Family and domain databases

    Gene3Di 2.60.120.10. 3 hits.
    InterProi IPR001250. Man6P_Isoase-1.
    IPR016305. Mannose-6-P_Isomerase.
    IPR018050. Pmannose_isomerase-type1_CS.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view ]
    PANTHERi PTHR10309. PTHR10309. 1 hit.
    Pfami PF01238. PMI_typeI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
    PRINTSi PR00714. MAN6PISMRASE.
    SUPFAMi SSF51182. SSF51182. 1 hit.
    TIGRFAMsi TIGR00218. manA. 1 hit.
    PROSITEi PS00965. PMI_I_1. 1 hit.
    PS00966. PMI_I_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of the Cryptococcus neoformans phosphomannose isomerase-encoding gene, MAN1, and its impact on pathogenicity."
      Wills E.A., Roberts I.S., Del Poeta M., Rivera J., Casadevall A., Cox G.M., Perfect J.R.
      Mol. Microbiol. 40:610-620(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans."
      Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S.
      , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.
      Science 307:1321-1324(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JEC21 / ATCC MYA-565.

    Entry informationi

    Entry nameiMPI_CRYNJ
    AccessioniPrimary (citable) accession number: Q9HFU4
    Secondary accession number(s): Q5KBJ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: June 21, 2005
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3