Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9HFU4 (MPI_CRYNJ) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannose-6-phosphate isomerase

EC=5.3.1.8
Alternative name(s):
Phosphohexomutase
Phosphomannose isomerase
Short name=PMI
Gene names
Name:MAN1
Ordered Locus Names:CNI02370
OrganismCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans) [Reference proteome]
Taxonomic identifier214684 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length434 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions By similarity.

Catalytic activity

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Pathway

Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the mannose-6-phosphate isomerase type 1 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMetal-binding
Zinc
   Molecular functionIsomerase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGDP-mannose biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmannose-6-phosphate isomerase activity

Inferred from electronic annotation. Source: UniProtKB-EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 434434Mannose-6-phosphate isomerase
PRO_0000194243

Sites

Active site3101 By similarity
Metal binding1091Zinc By similarity
Metal binding1111Zinc By similarity
Metal binding1361Zinc By similarity
Metal binding2911Zinc By similarity

Experimental info

Sequence conflict381S → A in AAG10203. Ref.1
Sequence conflict431K → R in AAG10203. Ref.1
Sequence conflict711K → R in AAG10203. Ref.1
Sequence conflict79 – 802SS → TT in AAG10203. Ref.1
Sequence conflict1541S → P in AAG10203. Ref.1
Sequence conflict1831G → D in AAG10203. Ref.1
Sequence conflict1921T → S in AAG10203. Ref.1
Sequence conflict2241L → H in AAG10203. Ref.1
Sequence conflict2371Q → K in AAG10203. Ref.1
Sequence conflict2481S → D in AAG10203. Ref.1
Sequence conflict3431P → S in AAG10203. Ref.1
Sequence conflict3961N → D in AAG10203. Ref.1
Sequence conflict400 – 4012RS → QT in AAG10203. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9HFU4 [UniParc].

Last modified June 21, 2005. Version 2.
Checksum: 4C2541EEFE9793FF

FASTA43447,590
        10         20         30         40         50         60 
MSPSVFKISP GINSYDWGKK GSASLAAQLA TTSIPDFSID EDKAYAELWM GTHPNNPSRL 

        70         80         90        100        110        120 
SDNTLLSEHL KSHPELIGSS VSSKFEDCKD GSLPFLFKVL SIGTALSIQA HPDKPLAKKL 

       130        140        150        160        170        180 
FDEKPDVYKD PNHKPEMAIA LTPFLAFLNF LPLSVLLLHL LTVPELQEFV DSSLTESLAS 

       190        200        210        220        230        240 
SLGLPTSQPP DTSLFKPTES PATAEQKDIL KQIFAALMSA DKKLVEEAIS KLIKRYQAKR 

       250        260        270        280        290        300 
DIKENEKSLV DLALRLNDQY PGDVGVLCVF LLNVVELKRG EAAFLGANEP HAYIEGDIIE 

       310        320        330        340        350        360 
CMATSDNVVR AGLTPKLRDV DTLVSMLTYE AAPGNKQLLQ PTPFQKGDDT TKLYDPPIAE 

       370        380        390        400        410        420 
FSVLRTELSK GMKTSHRPVE GPSLCVITEG EGVVRNGNDR SEFVRGDVIF VGAGKEVEWE 

       430 
AIKGLEMFRA YVEA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF291701 Genomic DNA. Translation: AAG10203.1.
AE017349 Genomic DNA. Translation: AAW45398.1.
RefSeqXP_572705.1. XM_572705.1.

3D structure databases

ProteinModelPortalQ9HFU4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING214684.CNI02370.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiAAW45398; AAW45398; CNI02370.
GeneID3259465.
KEGGcne:CNI02370.

Phylogenomic databases

eggNOGCOG1482.
KOK01809.
OrthoDBEOG7WDNCR.

Enzyme and pathway databases

UniPathwayUPA00126; UER00423.

Family and domain databases

Gene3D2.60.120.10. 3 hits.
InterProIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERPTHR10309. PTHR10309. 1 hit.
PfamPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSPR00714. MAN6PISMRASE.
SUPFAMSSF51182. SSF51182. 1 hit.
TIGRFAMsTIGR00218. manA. 1 hit.
PROSITEPS00965. PMI_I_1. 1 hit.
PS00966. PMI_I_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPI_CRYNJ
AccessionPrimary (citable) accession number: Q9HFU4
Secondary accession number(s): Q5KBJ0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: June 21, 2005
Last modified: November 13, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways