Q9HFS9 (ABNA_ASPAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arabinan endo-1,5-alpha-L-arabinosidase A
EC=3.2.1.99

Alternative name(s):
Endo-1,5-alpha-L-arabinanase A
Short name=ABN A

Gene names

Name:	abnA
Synonyms:	ara1

Organism

Aspergillus aculeatus

Taxonomic identifier

5053 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endo-1,5-alpha-L-arabinanase involved in degradation of pectin. Its preferred substrate is linear 1,5-alpha-L-arabinan By similarity. Ref.1
Catalytic activity	Endohydrolysis of (1->5)-alpha-arabinofuranosidic linkages in (1->5)-arabinans.
Pathway	Glycan metabolism; L-arabinan degradation.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 43 family.
Biophysicochemical properties	Kinetic parameters: KM=66 mM for AZCL-debranched arabinan Ref.1 pH dependence: Optimum pH is 5.5. Stable between pH 5.5 and 6.5. Temperature dependence: Optimum temperature is 50 degrees Celsius.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation Xylan degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	arabinan catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	arabinan endo-1,5-alpha-L-arabinosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 321	302	Arabinan endo-1,5-alpha-L-arabinosidase A		PRO_5000058634
Amino acid modifications
	Glycosylation	192	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9HFS9 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: FAE9D565631613F1	FASTA	321	34,097
	10 20 30 40 50 60 MYSLLTALSV PLLAGLAHGY ANPGSCSGSC NVHDPALIVR ESDGKYFRFS TGNEISYASA 70 80 90 100 110 120 SSINGPWTAI GSVVPAGSKI DLSGNTDLWA PDLSYVDGTY YCLYSVSTFG SQDSAIGVAS 130 140 150 160 170 180 STTMELNTWT DHGSVGVASS SSKNYNAIDG NLLVDGSSYY LQFGSFWGDI YQVKMASPLK 190 200 210 220 230 240 TAGSASYNIA YNATGTHSEE GSYLFKYGSY YYLFFSSGTC CGYDTSRPAQ GEEYKIMVCR 250 260 270 280 290 300 STSATGGFVD KNGNACTESG GTIVLASHGT VYGPGGQGVY DDPTYGPVLY YHYVDTTIGY 310 320 ADDQKLFGWN TIDFSSGWPV V

References

[1]

"Functional cloning of an endo-arabinanase from Aspergillus aculeatus and its heterologous expression in A. oryzae and tobacco." Skjoet M., Kauppinen S., Kofod L.V., Fuglsang C.C., Pauly M., Dalboege H., Andersen L.N. Mol. Genet. Genomics 265:913-921(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-38, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: CBS 101.43.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF300878 mRNA. Translation: AAG27441.1.
3D structure databases
ProteinModelPortal	Q9HFS9.
ModBase	Search...
Protein family/group databases
CAZy	GH43. Glycoside Hydrolase Family 43.
mycoCLAP	ABN43A_ASPAC.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00667.
Family and domain databases
Gene3D	2.115.10.20. 1 hit.
InterPro	IPR006710. Glyco_hydro_43. IPR016840. Glyco_hydro_43_endo_a_Ara-ase. IPR023296. Glyco_hydro_beta-prop. [Graphical view]
PANTHER	PTHR22925. PTHR22925. 1 hit.
Pfam	PF04616. Glyco_hydro_43. 1 hit. [Graphical view]
PIRSF	PIRSF026534. Endo_alpha-L-arabinosidase. 1 hit.
SUPFAM	SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ABNA_ASPAC

Accession

Primary (citable) accession number: Q9HFS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	March 1, 2001
Last modified:	May 29, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program