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Protein

Arabinan endo-1,5-alpha-L-arabinosidase A

Gene

abnA

Organism
Aspergillus aculeatus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Endo-1,5-alpha-L-arabinanase involved in degradation of pectin. Its preferred substrate is linear 1,5-alpha-L-arabinan (By similarity).By similarity

Catalytic activityi

Endohydrolysis of (1->5)-alpha-arabinofuranosidic linkages in (1->5)-arabinans.

Kineticsi

  1. KM=66 mM for AZCL-debranched arabinan1 Publication

    pH dependencei

    Optimum pH is 5.5. Stable between pH 5.5 and 6.5.1 Publication

    Temperature dependencei

    Optimum temperature is 50 degrees Celsius.1 Publication

    Pathwayi

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

    Enzyme and pathway databases

    UniPathwayiUPA00667.

    Protein family/group databases

    CAZyiGH43. Glycoside Hydrolase Family 43.
    mycoCLAPiABN43A_ASPAC.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arabinan endo-1,5-alpha-L-arabinosidase A (EC:3.2.1.99)
    Alternative name(s):
    Endo-1,5-alpha-L-arabinanase A
    Short name:
    ABN A
    Gene namesi
    Name:abnA
    Synonyms:ara1
    OrganismiAspergillus aculeatus
    Taxonomic identifieri5053 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 321302Arabinan endo-1,5-alpha-L-arabinosidase APRO_5000058634Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi192 – 1921N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HFS9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 43 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.115.10.20. 1 hit.
    InterProiIPR006710. Glyco_hydro_43.
    IPR016840. Glyco_hydro_43_endo_a_Ara-ase.
    IPR023296. Glyco_hydro_beta-prop.
    [Graphical view]
    PANTHERiPTHR22925. PTHR22925. 1 hit.
    PfamiPF04616. Glyco_hydro_43. 1 hit.
    [Graphical view]
    PIRSFiPIRSF026534. Endo_alpha-L-arabinosidase. 1 hit.
    SUPFAMiSSF75005. SSF75005. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9HFS9-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MYSLLTALSV PLLAGLAHGY ANPGSCSGSC NVHDPALIVR ESDGKYFRFS
    60 70 80 90 100
    TGNEISYASA SSINGPWTAI GSVVPAGSKI DLSGNTDLWA PDLSYVDGTY
    110 120 130 140 150
    YCLYSVSTFG SQDSAIGVAS STTMELNTWT DHGSVGVASS SSKNYNAIDG
    160 170 180 190 200
    NLLVDGSSYY LQFGSFWGDI YQVKMASPLK TAGSASYNIA YNATGTHSEE
    210 220 230 240 250
    GSYLFKYGSY YYLFFSSGTC CGYDTSRPAQ GEEYKIMVCR STSATGGFVD
    260 270 280 290 300
    KNGNACTESG GTIVLASHGT VYGPGGQGVY DDPTYGPVLY YHYVDTTIGY
    310 320
    ADDQKLFGWN TIDFSSGWPV V
    Length:321
    Mass (Da):34,097
    Last modified:March 1, 2001 - v1
    Checksum:iFAE9D565631613F1
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF300878 mRNA. Translation: AAG27441.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF300878 mRNA. Translation: AAG27441.1.

    3D structure databases

    ProteinModelPortaliQ9HFS9.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    CAZyiGH43. Glycoside Hydrolase Family 43.
    mycoCLAPiABN43A_ASPAC.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    UniPathwayiUPA00667.

    Family and domain databases

    Gene3Di2.115.10.20. 1 hit.
    InterProiIPR006710. Glyco_hydro_43.
    IPR016840. Glyco_hydro_43_endo_a_Ara-ase.
    IPR023296. Glyco_hydro_beta-prop.
    [Graphical view]
    PANTHERiPTHR22925. PTHR22925. 1 hit.
    PfamiPF04616. Glyco_hydro_43. 1 hit.
    [Graphical view]
    PIRSFiPIRSF026534. Endo_alpha-L-arabinosidase. 1 hit.
    SUPFAMiSSF75005. SSF75005. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    1. "Functional cloning of an endo-arabinanase from Aspergillus aculeatus and its heterologous expression in A. oryzae and tobacco."
      Skjoet M., Kauppinen S., Kofod L.V., Fuglsang C.C., Pauly M., Dalboege H., Andersen L.N.
      Mol. Genet. Genomics 265:913-921(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-38, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: CBS 101.43.

    Entry informationi

    Entry nameiABNA_ASPAC
    AccessioniPrimary (citable) accession number: Q9HFS9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 15, 2010
    Last sequence update: March 1, 2001
    Last modified: January 7, 2015
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.