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Q9HFS7

- UBP4_KLULA

UniProt

Q9HFS7 - UBP4_KLULA

Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene

DOA4

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Enzyme regulationi

    RFU1 is an inhibitor of deubiquitination activity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei441 – 4411NucleophilePROSITE-ProRule annotation
    Active sitei752 – 7521Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 4
    Ubiquitin thioesterase 4
    Ubiquitin-specific-processing protease 4
    Gene namesi
    Name:DOA4
    Synonyms:UBP4
    Ordered Locus Names:KLLA0E10275g
    OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
    Taxonomic identifieri284590 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
    ProteomesiUP000000598: Chromosome E

    Subcellular locationi

    Cytoplasm By similarity. Late endosome membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. late endosome membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 796796Ubiquitin carboxyl-terminal hydrolase 4PRO_0000376820Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HFS7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini170 – 294125RhodanesePROSITE-ProRule annotationAdd
    BLAST
    Domaini432 – 794363USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 rhodanese domain.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5533.
    HOGENOMiHOG000248489.
    KOiK11839.
    OMAiEDLNQCG.
    OrthoDBiEOG7R2BSX.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR001763. Rhodanese-like_dom.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00581. Rhodanese. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view]
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HFS7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLEHNSKLFC KSLSQLSAVA SKVVAEDVEG EHFKQLLAKC IDTLSIYKSE    50
    LRKLSCASKD TPPSQIYQLN ETLYVYYKIV SQIASQVIPG LAEFQQIKMN 100
    SKKDSKDKEL LEIYSRLVSA LANDKQIGEV KRFIKNHSEE AAHDGTQHSY 150
    ENGEFVSISQ LHSLIRHDND SSGILLVDIR PRMDFNDGHI KHNNVICIEP 200
    ISFKESYTDS DILRKSLITA SDREVDLFKN RDKFRLIVLY TDTDEHTKYY 250
    WQQLEVLQDI LCNRSFDKPL HHTKVIVLQN GVNAWKEKYP MELKKIMESA 300
    ISDIRTKPVE HNFHPMALSN NNNIEKANSP SHSQTTSFTH YPDAPFLTNG 350
    ASIKTAGLVP NQLLPSTSNL TKALQQNDEG NSEPYKLQPN GIKNHRQESN 400
    NHANGTNCIV SSNGNNSVAK SGHPSINLDF AIGLVNLGNS CYLNCIIQCL 450
    LGCHELSYIF LTNSYRKHVN VNSRLGSKGL LANYFSQLVQ KMYQQGKLQA 500
    YNNTNMESTA VHPTQFKLAC GSINSLFKGK QQQDCQEFCQ FLLDGLHEDL 550
    NQCGTNPPLK ELSPEAEKMR ETMPMRIASA IEWERYLTTD FSVIVDLFQG 600
    QYASQLRCKI CAHTSTTYQA FSVLSVPVPR ARSCTIYDCF KEFTKLETLE 650
    KDELWYCPYC KQRQPSTKQI IITRLPNNLI IHLKRFDNMM NKNNVFVNYP 700
    NELDLTGFWI DDYNGEMPKD NGVSLPLRGQ KPPFNYRLFA VASHSGTLYG 750
    GHYTSFVDKG RVGWCSFDDV SWRKIRRKDE YITPNAYVLF YRRVNM 796
    Length:796
    Mass (Da):90,929
    Last modified:March 1, 2001 - v1
    Checksum:i09A9F905579C14B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF303215 Genomic DNA. Translation: AAG17929.1.
    CR382125 Genomic DNA. Translation: CAG99500.1.
    RefSeqiXP_454413.1. XM_454413.1.

    Genome annotation databases

    GeneIDi2894137.
    KEGGikla:KLLA0E10275g.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF303215 Genomic DNA. Translation: AAG17929.1 .
    CR382125 Genomic DNA. Translation: CAG99500.1 .
    RefSeqi XP_454413.1. XM_454413.1.

    3D structure databases

    ProteinModelPortali Q9HFS7.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2894137.
    KEGGi kla:KLLA0E10275g.

    Phylogenomic databases

    eggNOGi COG5533.
    HOGENOMi HOG000248489.
    KOi K11839.
    OMAi EDLNQCG.
    OrthoDBi EOG7R2BSX.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR001763. Rhodanese-like_dom.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00581. Rhodanese. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view ]
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the deubiquitinating enzymes of the yeast Saccharomyces cerevisiae."
      Amerik A.Y., Li S.J., Hochstrasser M.
      Biol. Chem. 381:981-992(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Genome evolution in yeasts."
      Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
      , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
      Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37.

    Entry informationi

    Entry nameiUBP4_KLULA
    AccessioniPrimary (citable) accession number: Q9HFS7
    Secondary accession number(s): Q6CNS6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2009
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3