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Q9HFS7 (UBP4_KLULA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 4

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene names
Name:DOA4
Synonyms:UBP4
Ordered Locus Names:KLLA0E10275g
OrganismKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]
Taxonomic identifier284590 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces

Protein attributes

Sequence length796 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulation

RFU1 is an inhibitor of deubiquitination activity By similarity.

Subcellular location

Cytoplasm By similarity. Late endosome membrane; Peripheral membrane protein By similarity.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 rhodanese domain.

Contains 1 USP domain.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCytoplasm
Endosome
Membrane
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentlate endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 796796Ubiquitin carboxyl-terminal hydrolase 4
PRO_0000376820

Regions

Domain170 – 294125Rhodanese
Domain432 – 794363USP

Sites

Active site4411Nucleophile By similarity
Active site7521Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9HFS7 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 09A9F905579C14B2

FASTA79690,929
        10         20         30         40         50         60 
MLEHNSKLFC KSLSQLSAVA SKVVAEDVEG EHFKQLLAKC IDTLSIYKSE LRKLSCASKD 

        70         80         90        100        110        120 
TPPSQIYQLN ETLYVYYKIV SQIASQVIPG LAEFQQIKMN SKKDSKDKEL LEIYSRLVSA 

       130        140        150        160        170        180 
LANDKQIGEV KRFIKNHSEE AAHDGTQHSY ENGEFVSISQ LHSLIRHDND SSGILLVDIR 

       190        200        210        220        230        240 
PRMDFNDGHI KHNNVICIEP ISFKESYTDS DILRKSLITA SDREVDLFKN RDKFRLIVLY 

       250        260        270        280        290        300 
TDTDEHTKYY WQQLEVLQDI LCNRSFDKPL HHTKVIVLQN GVNAWKEKYP MELKKIMESA 

       310        320        330        340        350        360 
ISDIRTKPVE HNFHPMALSN NNNIEKANSP SHSQTTSFTH YPDAPFLTNG ASIKTAGLVP 

       370        380        390        400        410        420 
NQLLPSTSNL TKALQQNDEG NSEPYKLQPN GIKNHRQESN NHANGTNCIV SSNGNNSVAK 

       430        440        450        460        470        480 
SGHPSINLDF AIGLVNLGNS CYLNCIIQCL LGCHELSYIF LTNSYRKHVN VNSRLGSKGL 

       490        500        510        520        530        540 
LANYFSQLVQ KMYQQGKLQA YNNTNMESTA VHPTQFKLAC GSINSLFKGK QQQDCQEFCQ 

       550        560        570        580        590        600 
FLLDGLHEDL NQCGTNPPLK ELSPEAEKMR ETMPMRIASA IEWERYLTTD FSVIVDLFQG 

       610        620        630        640        650        660 
QYASQLRCKI CAHTSTTYQA FSVLSVPVPR ARSCTIYDCF KEFTKLETLE KDELWYCPYC 

       670        680        690        700        710        720 
KQRQPSTKQI IITRLPNNLI IHLKRFDNMM NKNNVFVNYP NELDLTGFWI DDYNGEMPKD 

       730        740        750        760        770        780 
NGVSLPLRGQ KPPFNYRLFA VASHSGTLYG GHYTSFVDKG RVGWCSFDDV SWRKIRRKDE 

       790 
YITPNAYVLF YRRVNM 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF303215 Genomic DNA. Translation: AAG17929.1.
CR382125 Genomic DNA. Translation: CAG99500.1.
RefSeqXP_454413.1. XM_454413.1.

3D structure databases

ProteinModelPortalQ9HFS7.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2894137.
KEGGkla:KLLA0E10275g.

Phylogenomic databases

eggNOGCOG5533.
HOGENOMHOG000248489.
KOK11839.
OMAEDLNQCG.
OrthoDBEOG7R2BSX.

Family and domain databases

Gene3D3.40.250.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMSSF52821. SSF52821. 1 hit.
PROSITEPS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP4_KLULA
AccessionPrimary (citable) accession number: Q9HFS7
Secondary accession number(s): Q6CNS6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries