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Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene

DOA4

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

RFU1 is an inhibitor of deubiquitination activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei441NucleophilePROSITE-ProRule annotation1
Active sitei752Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene namesi
Name:DOA4
Synonyms:UBP4
Ordered Locus Names:KLLA0E10275g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome E

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003768201 – 796Ubiquitin carboxyl-terminal hydrolase 4Add BLAST796

Interactioni

Protein-protein interaction databases

STRINGi284590.XP_454413.1.

Structurei

3D structure databases

ProteinModelPortaliQ9HFS7.
SMRiQ9HFS7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini170 – 294RhodanesePROSITE-ProRule annotationAdd BLAST125
Domaini432 – 794USPAdd BLAST363

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Phylogenomic databases

eggNOGiKOG1868. Eukaryota.
ENOG410XP8T. LUCA.
HOGENOMiHOG000248489.
InParanoidiQ9HFS7.
KOiK11839.
OMAiQYLRASE.
OrthoDBiEOG092C5R71.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR036873. Rhodanese-like_dom_sf.
IPR018200. USP_CS.
IPR028889. USP_dom.
PfamiView protein in Pfam
PF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
SMARTiView protein in SMART
SM00450. RHOD. 1 hit.
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiView protein in PROSITE
PS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HFS7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEHNSKLFC KSLSQLSAVA SKVVAEDVEG EHFKQLLAKC IDTLSIYKSE
60 70 80 90 100
LRKLSCASKD TPPSQIYQLN ETLYVYYKIV SQIASQVIPG LAEFQQIKMN
110 120 130 140 150
SKKDSKDKEL LEIYSRLVSA LANDKQIGEV KRFIKNHSEE AAHDGTQHSY
160 170 180 190 200
ENGEFVSISQ LHSLIRHDND SSGILLVDIR PRMDFNDGHI KHNNVICIEP
210 220 230 240 250
ISFKESYTDS DILRKSLITA SDREVDLFKN RDKFRLIVLY TDTDEHTKYY
260 270 280 290 300
WQQLEVLQDI LCNRSFDKPL HHTKVIVLQN GVNAWKEKYP MELKKIMESA
310 320 330 340 350
ISDIRTKPVE HNFHPMALSN NNNIEKANSP SHSQTTSFTH YPDAPFLTNG
360 370 380 390 400
ASIKTAGLVP NQLLPSTSNL TKALQQNDEG NSEPYKLQPN GIKNHRQESN
410 420 430 440 450
NHANGTNCIV SSNGNNSVAK SGHPSINLDF AIGLVNLGNS CYLNCIIQCL
460 470 480 490 500
LGCHELSYIF LTNSYRKHVN VNSRLGSKGL LANYFSQLVQ KMYQQGKLQA
510 520 530 540 550
YNNTNMESTA VHPTQFKLAC GSINSLFKGK QQQDCQEFCQ FLLDGLHEDL
560 570 580 590 600
NQCGTNPPLK ELSPEAEKMR ETMPMRIASA IEWERYLTTD FSVIVDLFQG
610 620 630 640 650
QYASQLRCKI CAHTSTTYQA FSVLSVPVPR ARSCTIYDCF KEFTKLETLE
660 670 680 690 700
KDELWYCPYC KQRQPSTKQI IITRLPNNLI IHLKRFDNMM NKNNVFVNYP
710 720 730 740 750
NELDLTGFWI DDYNGEMPKD NGVSLPLRGQ KPPFNYRLFA VASHSGTLYG
760 770 780 790
GHYTSFVDKG RVGWCSFDDV SWRKIRRKDE YITPNAYVLF YRRVNM
Length:796
Mass (Da):90,929
Last modified:March 1, 2001 - v1
Checksum:i09A9F905579C14B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF303215 Genomic DNA. Translation: AAG17929.1.
CR382125 Genomic DNA. Translation: CAG99500.1.
RefSeqiXP_454413.1. XM_454413.1.

Genome annotation databases

EnsemblFungiiCAG99500; CAG99500; KLLA0_E10275g.
GeneIDi2894137.
KEGGikla:KLLA0E10275g.

Similar proteinsi

Entry informationi

Entry nameiUBP4_KLULA
AccessioniPrimary (citable) accession number: Q9HFS7
Secondary accession number(s): Q6CNS6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: March 1, 2001
Last modified: October 25, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families