Reviewed, UniProtKB/Swiss-Prot Q9HFR6 (DHE4_TUBBO)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase
      Short name=NADP-GDH
    EC=1.4.1.4
Alternative name(s):
    NADP-dependent glutamate dehydrogenase

Gene names

Name:

GDH

Organism

Tuber borchii (White truffle)

Taxonomic identifier

42251 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Pezizomycetes › Pezizales › Tuberaceae › Tuber

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	NADP-specific glutamate dehydrogenase		PRO_0000182795
Sites
	Active site	113	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HFR6-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: C32DE331D01DF612	FASTA	457	50,155
	10 20 30 40 50 60 MSNLAPEPEF QQAYNELVHS LRDQNSSRLP QILRLLCLSS PPERVIQFRV TWEDDKGNFQ 70 80 90 100 110 120 VNRGYRVQFN SALGPYKGGL RFHPTVNLSI LKFLGFEQTF KNALTGLNMG GGKGGSDFDP 130 140 150 160 170 180 KGKSDNEIRR FCYSFMRELS KHIGQFTDVP AGDIGVGGRE IGYLFGAYES YKNQFEGVLT 190 200 210 220 230 240 GKGITWGGSL IRPEATGYGL VYYVAHMISY ASGGKETFAG KRVAISGSGN VAQYAALKVL 250 260 270 280 290 300 ELGGKVITLS DSKGALIATG EEGFNETDIE LIAKLKLDRG YLTQLHAAED SFKSRFKYLP 310 320 330 340 350 360 GERPWCHVDK VDVALPSATQ NEVSEQEAKE LIASGCKFLA EGSNMGSTQE AINVYEEDRK 370 380 390 400 410 420 SRKADGLWYG PAKAANCGGV AVSGLEMAQN SQRLTWTSEQ VDKELAGIME RCFWNCLNPA 430 440 450 KEYFDIAEGE LPSLVAGANI AGYVKVVNAM KAQGDWW

References

[1]

"Biochemical and molecular characterization of NADP-glutamate dehydrogenase from the ectomycorrhizal fungus Tuber borchii." Vallorani L., Polidori E., Sacconi C., Agostini D., Pierleoni R., Piccoli G., Zeppa S., Stocchi V. New Phytol. 154:779-790(2002) [Agricola: IND23280867] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF309087 Genomic DNA. Translation: AAG28788.1.
3D structure databases
SMR	Q9HFR6. Positions 5-456.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.1.4. 280607.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_TUBBO

Accession

Primary (citable) accession number: Q9HFR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)