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Q9HFA4

- XYNA_ASPOR

UniProt

Q9HFA4 - XYNA_ASPOR

Protein

Endo-1,4-beta-xylanase A

Gene

xlnA

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.1 Publication

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.

    Kineticsi

    1. KM=242.8 µM for birch wood xylan1 Publication

    Vmax=123 µmol/min/mg enzyme1 Publication

    pH dependencei

    Optimum pH is 6.0. Retains over 95 percent activity in the pH range from 5.0 to 7.0, and 70 percent activity in the pH range from 4.0 to 8.0.1 Publication

    Temperature dependencei

    Optimum temperature is 58 degrees Celsius. Has complete stability at 60 degrees Celsius.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei128 – 1281NucleophilePROSITE-ProRule annotation
    Active sitei219 – 2191Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. endo-1,4-beta-xylanase activity Source: UniProtKB

    GO - Biological processi

    1. xylan catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16226.
    UniPathwayiUPA00114.

    Protein family/group databases

    CAZyiGH11. Glycoside Hydrolase Family 11.
    mycoCLAPiXYN11A_ASPOR.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-1,4-beta-xylanase A (EC:3.2.1.8)
    Short name:
    Xylanase A
    Alternative name(s):
    1,4-beta-D-xylan xylanohydrolase A
    Endo-1,4-beta-xylanase G2
    Short name:
    Xylanase G2
    Gene namesi
    Name:xlnA
    Synonyms:xlnG2, xynG2
    ORF Names:AO090120000026
    OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
    Taxonomic identifieri510516 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000006564: Chromosome 5

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: ASPGD

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 232213Endo-1,4-beta-xylanase APRO_0000393162Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi5062.CADAORAP00010980.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HFA4.
    SMRiQ9HFA4. Positions 46-231.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG05353.
    HOGENOMiHOG000179135.
    OMAiNETALHE.
    OrthoDBiEOG7VQJQX.

    Family and domain databases

    Gene3Di2.60.120.180. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR001137. Glyco_hydro_11.
    IPR013319. Glyco_hydro_11/12.
    IPR018208. Glyco_hydro_11_AS.
    [Graphical view]
    PfamiPF00457. Glyco_hydro_11. 1 hit.
    [Graphical view]
    PRINTSiPR00911. GLHYDRLASE11.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS00776. GLYCOSYL_HYDROL_F11_1. 1 hit.
    PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9HFA4-1 [UniParc]FASTAAdd to Basket

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    MVSFSSILLA CSAAIGALAT PIEPLADHPN EAFNETAFND LVGRSTPSST    50
    GYNNGYYYSF WTDGGGDVTY TNGNGGSYSV QWSNVGNFVG GKGWNPGSSR 100
    AITYSGSFNP SGNGYLAVYG WTTDPLIEYY IVESYGTYNP GSGGTYKGQV 150
    TSDGGTYNIY TSVRTNAPSI IGTATFTQFW SVRTSKRVGG TVTTGNHFNA 200
    WAKYGLTLGT HNYQIVATEG YQSSGSSAIT VY 232
    Length:232
    Mass (Da):24,605
    Last modified:March 1, 2001 - v1
    Checksum:i1F73104751EA561C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU848307 mRNA. Translation: ACJ26384.1.
    AB044941 Genomic DNA. Translation: BAB20794.1.
    AP007166 Genomic DNA. Translation: BAE62665.1.
    PIRiJC7577.
    RefSeqiXP_001823798.1. XM_001823746.2.

    Genome annotation databases

    EnsemblFungiiCADAORAT00011206; CADAORAP00010980; CADAORAG00011206.
    GeneIDi5996057.
    KEGGiaor:AOR_1_38094.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU848307 mRNA. Translation: ACJ26384.1 .
    AB044941 Genomic DNA. Translation: BAB20794.1 .
    AP007166 Genomic DNA. Translation: BAE62665.1 .
    PIRi JC7577.
    RefSeqi XP_001823798.1. XM_001823746.2.

    3D structure databases

    ProteinModelPortali Q9HFA4.
    SMRi Q9HFA4. Positions 46-231.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5062.CADAORAP00010980.

    Protein family/group databases

    CAZyi GH11. Glycoside Hydrolase Family 11.
    mycoCLAPi XYN11A_ASPOR.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADAORAT00011206 ; CADAORAP00010980 ; CADAORAG00011206 .
    GeneIDi 5996057.
    KEGGi aor:AOR_1_38094.

    Phylogenomic databases

    eggNOGi NOG05353.
    HOGENOMi HOG000179135.
    OMAi NETALHE.
    OrthoDBi EOG7VQJQX.

    Enzyme and pathway databases

    UniPathwayi UPA00114 .
    BioCyci MetaCyc:MONOMER-16226.

    Family and domain databases

    Gene3Di 2.60.120.180. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR001137. Glyco_hydro_11.
    IPR013319. Glyco_hydro_11/12.
    IPR018208. Glyco_hydro_11_AS.
    [Graphical view ]
    Pfami PF00457. Glyco_hydro_11. 1 hit.
    [Graphical view ]
    PRINTSi PR00911. GLHYDRLASE11.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS00776. GLYCOSYL_HYDROL_F11_1. 1 hit.
    PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, overexpression, and purification of a major xylanase from Aspergillus oryzae."
      Kimura T., Suzuki H., Furuhashi H., Aburatani T., Morimoto K., Karita S., Sakka K., Ohmiya K.
      Biosci. Biotechnol. Biochem. 64:2734-2738(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 45-64, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    2. "Gene cloning, sequencing, expression and characterization of a beta-xylanase gene from Aspergillus oryzae VTCC-F187."
      Quyen D.T., Nguyen S.L.T.
      Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: VTCC-F-187.
    3. "Genome sequencing and analysis of Aspergillus oryzae."
      Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
      , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
      Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 42149 / RIB 40.
    4. "Proteomic analysis of extracellular proteins from Aspergillus oryzae grown under submerged and solid-state culture conditions."
      Oda K., Kakizono D., Yamada O., Iefuji H., Akita O., Iwashita K.
      Appl. Environ. Microbiol. 72:3448-3457(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiXYNA_ASPOR
    AccessioniPrimary (citable) accession number: Q9HFA4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 23, 2010
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The promoter contains two 5'-GGCTAAA-3' sequences identified as binding sites for the xylanolytic transcriptional activator xlnR.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3