Q9HEW1 (KAPR_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
cAMP-dependent protein kinase regulatory subunit
Short name=PKA regulatory subunit
Short name=PKA-R

Gene names

Name:	BCY1
Synonyms:	PKAR
ORF Names:	CaO19.9565

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Subunit structure	Tetramer, composed of 2 regulatory (R) and 2 catalytic (C) subunits. In the presence of cAMP it dissociates into 2 active monomeric C subunits and an R dimer By similarity.
Sequence similarities	Belongs to the cAMP-dependent kinase regulatory chain family. Contains 2 cyclic nucleotide-binding domains.

Ontologies

Keywords
Domain	Repeat
Ligand	Nucleotide-binding cAMP cAMP-binding
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	regulation of protein phosphorylation Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cAMP-dependent protein kinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	cAMP binding Inferred from electronic annotation. Source: UniProtKB-KW cAMP-dependent protein kinase regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	cAMP-dependent protein kinase regulatory subunit		PRO_0000205404
Regions
	Nucleotide binding	220 – 335	116	cAMP 1
	Nucleotide binding	338 – 454	117	cAMP 2
	Region	30 – 219	190	Dimerization and phosphorylation Potential
Sites
	Binding site	285	1	cAMP 1 By similarity
	Binding site	294	1	cAMP 1 By similarity
	Binding site	404	1	cAMP 2 By similarity
	Binding site	413	1	cAMP 2 By similarity
Amino acid modifications
	Modified residue	180	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HEW1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 68E22227601A1060	FASTA	459	50,306
	10 20 30 40 50 60 MSNPQQQFIS DELSQLQKEI ISKNPQDVLQ FCANYFNTKL QAQRSELWSQ QAKAEAAGID 70 80 90 100 110 120 LFPSVDHVNV NSSGVSIVND RQPSFKSPFG VNDPHSNHDE DPHAKDTKTD TAAAAVGGGI 130 140 150 160 170 180 FKSNFDVKKS ASNPPTKEVD PDDPSKPSSS SQPNQQSASA SSKTPSSKIP VAFNANRRTS 190 200 210 220 230 240 VSAEALNPAK LKLDSWKPPV NNLSITEEET LANNLKNNFL FKQLDANSKK TVIAALQQKS 250 260 270 280 290 300 FAKDTVIIQQ GDEGDFFYII ETGTVDFYVN DAKVSSSSEG SSFGELALMY NSPRAATAVA 310 320 330 340 350 360 ATDVVCWALD RLTFRRILLE GTFNKRLMYE DFLKDIEVLK SLSDHARSKL ADALSTEMYH 370 380 390 400 410 420 KGDKIVTEGE QGENFYLIES GNCQVYNEKL GNIKQLTKGD YFGELALIKD LPRQATVEAL 430 440 450 DNVIVATLGK SGFQRLLGPV VEVLKEQDPT KSQDPTAGH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Candida albicans lacking the gene encoding the regulatory subunit of protein kinase A displays a defect in hyphal formation and an altered localization of the catalytic subunit." Cassola A., Parrot M., Silberstein S., Magee B.B., Passeron S., Giasson L., Cantore M.L. Eukaryot. Cell 3:190-199(2004) [PubMed: 14871949] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CAI4.
[2]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF317472 Genomic DNA. Translation: AAG38599.1. AACQ01000030 Genomic DNA. Translation: EAL00712.1.
RefSeq	XP_719591.1. XM_714498.1.
3D structure databases
ProteinModelPortal	Q9HEW1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9HEW1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3638765.
KEGG	cal:CaO19.9565.
Organism-specific databases
CGD	CAL0069592. BCY1.
Phylogenomic databases
OMA	NANRRTS.
PhylomeDB	Q9HEW1.
Family and domain databases
InterPro	IPR002373. cAMP/cGMP_kin. IPR012198. cAMP_dep_PK_reg_su. IPR003117. cAMP_dep_PK_reg_su_I/II_a/b. IPR018490. cNMP-bd-like. IPR018488. cNMP-bd_CS. IPR000595. cNMP-bd_dom. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
KO	K04739.
Pfam	PF00027. cNMP_binding. 2 hits. PF02197. RIIa. 1 hit. [Graphical view]
PIRSF	PIRSF000548. PK_regulatory. 1 hit.
PRINTS	PR00103. CAMPKINASE.
SMART	SM00100. cNMP. 2 hits. [Graphical view]
SUPFAM	SSF51206. cNMP_binding. 2 hits.
PROSITE	PS00888. CNMP_BINDING_1. 2 hits. PS00889. CNMP_BINDING_2. 2 hits. PS50042. CNMP_BINDING_3. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAPR_CANAL

Accession

Primary (citable) accession number: Q9HEW1
Secondary accession number(s): Q5ADI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program