Reviewed, UniProtKB/Swiss-Prot Q9HET9 (TPP1_SACMI)

Names and origin

Protein names

Recommended name:
    Polynucleotide 3'-phosphatase
    EC=3.1.3.32
Alternative name(s):
    2'(3')-polynucleotidase
    DNA 3'-phosphatase
    Three prime phosphatase

Gene names

Name:

TPP1

Organism

Saccharomyces mikatae (Yeast)

Taxonomic identifier

114525 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Dephosphorylate DNA's 3'-phosphate termini. Has a role in the repair of breaks in single stranded DNA.
Catalytic activity	A 3'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the DNA 3' phosphatase family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polynucleotide 3'-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Polynucleotide 3'-phosphatase		PRO_0000065579

Sequences

	Sequence		Length	Mass (Da)
	Q9HET9-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 4C58256B8B1149EA	FASTA	245	28,144
	10 20 30 40 50 60 MKTEGRQMPH KSTILPFLIK FAPNSPQFTY DCDQCLNVYA FDLDHTIIKP KSPNAKYSRS 70 80 90 100 110 120 ANDWQFMNFD SKKSTLDYLF SITDNDLAAV IVIFSNQGGV ITVPRTSKSC SKYINKISLF 130 140 150 160 170 180 LKAIENDKRG EKLSPRLWIY AAPKRPKTVV TNNKKITFPS LCKSYNNDPE IFEKVRKPMT 190 200 210 220 230 240 GMADFFRIDI TDACRVLKTV PPIKLNWVYY CGDAAGRKND FSDSDIKFAE KLHVEFKYPE EVFQG

References

[1]

"Uncoupling of 3'-phosphatase and 5'-kinase functions in budding yeast. Characterization of Saccharomyces cerevisiae DNA 3'-phosphatase (TPP1)." Vance J.R., Wilson T.E. J. Biol. Chem. 276:15073-15081(2001) [PubMed: 11278831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IFO 1815 / NCYC 2888.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF326782 Genomic DNA. Translation: AAG45938.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.3.32. 278541.
Family and domain databases
InterPro	IPR006551. DNA-3-Pase. IPR013954. PNK3P_central-region. IPR015636. PNK_3Pase. [Graphical view]
PANTHER	PTHR12083. PNK_3Pase. 1 hit.
Pfam	PF08645. PNK3P. 1 hit. [Graphical view]
TIGRFAMs	TIGR01664. DNA-3_-Pase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPP1_SACMI

Accession

Primary (citable) accession number: Q9HET9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)